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题名: An in vitro system to study cyclopeptide heterophyllin B biosynthesis in the medicinal plant Pseudostellaria heterophylla
作者: Xu, Wenyan1, 2, 3; Zhu, Hongtao2; Tan, Ninghua2; Tang, Jun2; null(张颖君)2; Cerny, Ron L.1; Du, Liangcheng1
刊名: PLANT CELL TISSUE AND ORGAN CULTURE
关键词: Heterophyllin B ; Plant cyclopeptide ; Biosynthesis ; Pseudostellaria heterophylla ; Traditional Chinese medicine
英文摘要: Plant cyclopeptides are a large group of small molecule metabolites found in a wide variety of plants, including traditional Chinese medicinal plants. Many of the cyclopeptides have highly unusual structures and potent biological activities. However, the majority of the cyclopeptides have not been studied for their biosynthetic mechanisms. In this study, we have established a culture system for the biosynthetic study of heterophyllin B (HB), a cyclopeptide produced by the medicinal plant Pseudostellaria heterophylla. We first developed a shoot culture of P. heterophylla that produced HB consistently under laboratory conditions. Using 14 C-labeled proline as tracer, we showed that labeled HB was produced by the cultured shoots, indicating that this system has de novo biosynthetic activity. Next, we chemically synthesized HB's linear peptide precursor (LHB) and the N-acetyl cysteamine thioester of LHB (LHB-SNAC). When LHB-SNAC was incubated with total cell free extracts of the cultured shoots, a small amount of cyclized product (HB), in addition to the hydrolyzed product (LHB), was produced. The in vivo and in vitro results demonstrate the presence of an HB biosynthetic system, which provides insight into the molecular mechanism for plant cyclopeptide biosynthesis.
出版日期: 2012
卷号: 108, 期号:1, 页码:137-145
DOI标识: 10.1007/s11240-011-0022-8
语种: 英语
ISSN号: 0167-6857
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/10447
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

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作者单位: 1.Univ Nebraska, Dept Chem, Lincoln, NE 68588 USA
2.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Xu, WY; Zhu, HT; Tan, NH; Tang, J; Zhang, YJ; Cerny, RL; Du, LC.An in vitro system to study cyclopeptide heterophyllin B biosynthesis in the medicinal plant Pseudostellaria heterophylla,PLANT CELL TISSUE AND ORGAN CULTURE,2012,108(1):137-145
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