An in vitro system to study cyclopeptide heterophyllin B biosynthesis in the medicinal plant Pseudostellaria heterophylla
Xu, Wenyan1,2,3; Zhu, Hongtao2; Tan, Ninghua2; Tang, Jun2; Zhang, Yingjun2; Cerny, Ron L.1; Du, Liangcheng1
2012
发表期刊PLANT CELL TISSUE AND ORGAN CULTURE
ISSN0167-6857
卷号108期号:1页码:137-145
摘要Plant cyclopeptides are a large group of small molecule metabolites found in a wide variety of plants, including traditional Chinese medicinal plants. Many of the cyclopeptides have highly unusual structures and potent biological activities. However, the majority of the cyclopeptides have not been studied for their biosynthetic mechanisms. In this study, we have established a culture system for the biosynthetic study of heterophyllin B (HB), a cyclopeptide produced by the medicinal plant Pseudostellaria heterophylla. We first developed a shoot culture of P. heterophylla that produced HB consistently under laboratory conditions. Using 14 C-labeled proline as tracer, we showed that labeled HB was produced by the cultured shoots, indicating that this system has de novo biosynthetic activity. Next, we chemically synthesized HB's linear peptide precursor (LHB) and the N-acetyl cysteamine thioester of LHB (LHB-SNAC). When LHB-SNAC was incubated with total cell free extracts of the cultured shoots, a small amount of cyclized product (HB), in addition to the hydrolyzed product (LHB), was produced. The in vivo and in vitro results demonstrate the presence of an HB biosynthetic system, which provides insight into the molecular mechanism for plant cyclopeptide biosynthesis.
关键词Heterophyllin b Plant Cyclopeptide Biosynthesis Pseudostellaria Heterophylla Traditional Chinese Medicine
资助信息Nebraska Research Council; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences; China Scholarship Council; NIH[RR015468-01]; NSFC[30725048]
收录类别SCI
语种英语
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/10447
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Univ Nebraska, Dept Chem, Lincoln, NE 68588 USA
2.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
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Xu, Wenyan,Zhu, Hongtao,Tan, Ninghua,et al. An in vitro system to study cyclopeptide heterophyllin B biosynthesis in the medicinal plant Pseudostellaria heterophylla[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2012,108(1):137-145.
APA Xu, Wenyan.,Zhu, Hongtao.,Tan, Ninghua.,Tang, Jun.,Zhang, Yingjun.,...&Du, Liangcheng.(2012).An in vitro system to study cyclopeptide heterophyllin B biosynthesis in the medicinal plant Pseudostellaria heterophylla.PLANT CELL TISSUE AND ORGAN CULTURE,108(1),137-145.
MLA Xu, Wenyan,et al."An in vitro system to study cyclopeptide heterophyllin B biosynthesis in the medicinal plant Pseudostellaria heterophylla".PLANT CELL TISSUE AND ORGAN CULTURE 108.1(2012):137-145.
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