Phenotyping of Tianma-Stimulated Differentiated Rat Neuronal B104 Cells by Quantitative Proteomics
Sundaramurthi, Husvinee; Manavalan, Arulmani2; Ramachandran, Umamaheswari2; Hu, Jiang-Miao3; Sze, Siu Kwan; Heese, Klaus1,2
2012
发表期刊NEUROSIGNALS
ISSN1424-862X
卷号20期号:1页码:48-60
摘要Gastrodia elata blume (tianma) is a traditional Chinese herb often used in the treatment of convulsions, headaches, and hypertension. Although interest in neuronal- related actions of tianma is increasing, minimal studies have been conducted to determine its specific effects on neuronal cells. This study was designed to examine the effects of tianma on the metabolism in differentiated neuroblastoma cells using the isobaric tag for relative and absolute quantitation (iTRAQ) technology. Stimulation of these cells with tianma caused changes in the expression of 38 proteins that were subsequently classified according to their physiological functions and association with neurodegenerative diseases. We identified six proteins with altered functional activities in neurodegenerative disease states that were modulated by tianma: triosephosphate isomerase (Tpi1), peptidyl- prolyl cis- trans isomerase A (Ppia), neural cell adhesion molecule 1 (Ncam1), ubiquitin carboxyl- terminal hydrolase isozyme L1 (Uchl1), septin- 2 (Sept2) and heat shock protein 90 (Hsp90aa1). Wepostulate that tianma mediates its neuroprotective effects via upregulation of Ncam1, Hsp90aa1, Tpi1 and Ppia while downregulating Sept2 and Uchl1. These changes in protein expression aid in the restoration of the intracellular environment to a metabolically balanced state, promoting cell survival. Based on these observed data, we conclude that tianma has therapeutic potential, especially for neurodegenerative diseases. Copyright (C) 2011 S. Karger AG, Basel
关键词Itraq Technology Neural Cell Adhesion Molecule 1 Neurodegenerative Diseases Tianma
资助信息Institute of Advanced Studies, Nanyang Technological University
收录类别SCI
语种英语
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/10423
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Nanyang Technol Univ, Sch Biol Sci, Dept Mol & Cell Biol, Singapore 637551, Singapore
2.Nanyang Technol Univ, Inst Adv Studies, Singapore 637551, Singapore
3.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China
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Sundaramurthi, Husvinee,Manavalan, Arulmani,Ramachandran, Umamaheswari,et al. Phenotyping of Tianma-Stimulated Differentiated Rat Neuronal B104 Cells by Quantitative Proteomics[J]. NEUROSIGNALS,2012,20(1):48-60.
APA Sundaramurthi, Husvinee,Manavalan, Arulmani,Ramachandran, Umamaheswari,Hu, Jiang-Miao,Sze, Siu Kwan,&Heese, Klaus.(2012).Phenotyping of Tianma-Stimulated Differentiated Rat Neuronal B104 Cells by Quantitative Proteomics.NEUROSIGNALS,20(1),48-60.
MLA Sundaramurthi, Husvinee,et al."Phenotyping of Tianma-Stimulated Differentiated Rat Neuronal B104 Cells by Quantitative Proteomics".NEUROSIGNALS 20.1(2012):48-60.
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