| alpha-Mangostin Induces Apoptosis and Suppresses Differentiation of 3T3-L1 Cells via Inhibiting Fatty Acid Synthase | |
| Quan, Xiaofang1; Wang, Yi1; Ma, Xiaofeng1; Liang, Yan1; Tian, Weixi1; Ma, Qingyun2; Jiang, Hezhong3; Zhao, Youxing2 | |
| 通讯作者 | Quan, XF (reprint author), Chinese Acad Sci, Grad Univ, Coll Life Sci, Beijing, Peoples R China, maxiaofeng@gucas.ac.cn ; zhaoyx1011@163.com |
| 2012-03-09 | |
| 发表期刊 | PLOS ONE
 |
| ISSN | 1932-6203 |
| 卷号 | 7期号:3页码:e33376 |
| 摘要 | alpha-Mangostin, isolated from the hulls of Garcinia mangostana L., was found to have in vitro cytotoxicity against 3T3-L1 cells as well as inhibiting fatty acid synthase (FAS, EC 2.3.1.85). Our studies showed that the cytotoxicity of alpha-mangostin with IC50 value of 20 mu M was incomplicated in apoptotic events including increase of cell membrane permeability, nuclear chromatin condensation and mitochondrial membrane potential (Delta Psi m) loss. This cytotoxicity was accompanied by the reduction of FAS activity in cells and could be rescued by 50 mM or 100 mM exogenous palmitic acids, which suggested that the apoptosis of 3T3-L1 preadipocytes induced by alpha-mangostin was via inhibition of FAS. Futhermore, alpha-mangostin could suppress intracellular lipid accumulation in the differentiating adipocytes and stimulated lipolysis in mature adipocytes, which was also related to its inhibition of FAS. In addition, 3T3-L1 preadipocytes were more susceptible to the cytotoxic effect of alpha-mangostin than mature adipocytes. Further studies showed that alpha-mangostin inhibited FAS probably by stronger action on the ketoacyl synthase domain and weaker action on the acetyl/malonyl transferase domain. These findings suggested that alpha-mangostin might be useful for preventing or treating obesity. |
| 关键词 | Aconitum Transsectum Diterpenoid Alkaloids Aconitramines d And e Norditerpenoid Alkaloids |
| 学科领域 | Biology |
| DOI | 10.1371/journal.pone.0033376 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000303062800054 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/10417 |
| 专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Grad Univ, Coll Life Sci, Beijing, Peoples R China 2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming, Peoples R China |
| 推荐引用方式 GB/T 7714 | Quan, Xiaofang,Wang, Yi,Ma, Xiaofeng,et al. alpha-Mangostin Induces Apoptosis and Suppresses Differentiation of 3T3-L1 Cells via Inhibiting Fatty Acid Synthase[J]. PLOS ONE,2012,7(3):e33376. |
| APA | Quan, Xiaofang.,Wang, Yi.,Ma, Xiaofeng.,Liang, Yan.,Tian, Weixi.,...&Zhao, Youxing.(2012).alpha-Mangostin Induces Apoptosis and Suppresses Differentiation of 3T3-L1 Cells via Inhibiting Fatty Acid Synthase.PLOS ONE,7(3),e33376. |
| MLA | Quan, Xiaofang,et al."alpha-Mangostin Induces Apoptosis and Suppresses Differentiation of 3T3-L1 Cells via Inhibiting Fatty Acid Synthase".PLOS ONE 7.3(2012):e33376. |
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