alpha-Mangostin Induces Apoptosis and Suppresses Differentiation of 3T3-L1 Cells via Inhibiting Fatty Acid Synthase
Quan, Xiaofang1; Wang, Yi1; Ma, Xiaofeng1; Liang, Yan1; Tian, Weixi1; Ma, Qingyun2; Jiang, Hezhong3; Zhao, Youxing2
2012-03-09
发表期刊PLOS ONE
ISSN1932-6203
卷号7期号:3页码:e33376
摘要alpha-Mangostin, isolated from the hulls of Garcinia mangostana L., was found to have in vitro cytotoxicity against 3T3-L1 cells as well as inhibiting fatty acid synthase (FAS, EC 2.3.1.85). Our studies showed that the cytotoxicity of alpha-mangostin with IC50 value of 20 mu M was incomplicated in apoptotic events including increase of cell membrane permeability, nuclear chromatin condensation and mitochondrial membrane potential (Delta Psi m) loss. This cytotoxicity was accompanied by the reduction of FAS activity in cells and could be rescued by 50 mM or 100 mM exogenous palmitic acids, which suggested that the apoptosis of 3T3-L1 preadipocytes induced by alpha-mangostin was via inhibition of FAS. Futhermore, alpha-mangostin could suppress intracellular lipid accumulation in the differentiating adipocytes and stimulated lipolysis in mature adipocytes, which was also related to its inhibition of FAS. In addition, 3T3-L1 preadipocytes were more susceptible to the cytotoxic effect of alpha-mangostin than mature adipocytes. Further studies showed that alpha-mangostin inhibited FAS probably by stronger action on the ketoacyl synthase domain and weaker action on the acetyl/malonyl transferase domain. These findings suggested that alpha-mangostin might be useful for preventing or treating obesity.
关键词Aconitum Transsectum Diterpenoid Alkaloids Aconitramines d And e Norditerpenoid Alkaloids
资助信息Special Science and Technology Projects for Outstanding Young in Life Sciences[KSCX2-EW-Q-19]; Twelfth Five Year Plan Basic Frontier Project in Life Sciences[Y129015EA2]; Natural Science Foundation of Yunnan[2008CD159]; GUCAS[O95101PY00]; K.C. Wong Education Foundation, Hong Kong
学科领域Biology
DOI10.1371/journal.pone.0033376
收录类别SCI
语种英语
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
WOS记录号WOS:000303062800054
引用统计
被引频次:29[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/10417
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Chinese Acad Sci, Grad Univ, Coll Life Sci, Beijing, Peoples R China
2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming, Peoples R China
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Quan, Xiaofang,Wang, Yi,Ma, Xiaofeng,et al. alpha-Mangostin Induces Apoptosis and Suppresses Differentiation of 3T3-L1 Cells via Inhibiting Fatty Acid Synthase[J]. PLOS ONE,2012,7(3):e33376.
APA Quan, Xiaofang.,Wang, Yi.,Ma, Xiaofeng.,Liang, Yan.,Tian, Weixi.,...&Zhao, Youxing.(2012).alpha-Mangostin Induces Apoptosis and Suppresses Differentiation of 3T3-L1 Cells via Inhibiting Fatty Acid Synthase.PLOS ONE,7(3),e33376.
MLA Quan, Xiaofang,et al."alpha-Mangostin Induces Apoptosis and Suppresses Differentiation of 3T3-L1 Cells via Inhibiting Fatty Acid Synthase".PLOS ONE 7.3(2012):e33376.
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