IeCPS2 is potentially involved in the biosynthesis pharmacologically active Isodon diterpenoids rather than gibberellin

doi:10.1016/j.phytochem.2011.12.021

	IeCPS2 is potentially involved in the biosynthesis pharmacologically active Isodon diterpenoids rather than gibberellin
	Li, Jun-Ling 1,3; Chen, Qian-Qian 1,3; Jin, Qiu-Ping 2; Gao, Juan 1,3; Zhao, Pei-Ji 1; Lu, Shan 2; Zeng, Ying1
通讯作者	Zeng, Y (reprint author), 132 Lanhei Rd, Kunming 650201, Peoples R China, biochem@mail.kib.ac.cn
	2012-04-01
发表期刊	PHYTOCHEMISTRY
ISSN	0031-9422
卷号	76 页码:32-39
摘要	The traditional Chinese medicinal plant, Isodon L, is remarkably rich in pharmacologically active ent-kaurane diterpenoids of diverse carbon skeletons. In an effort to create a resource for gene discovery and elucidate the biosynthesis of Isodon ent-kaurane diterpenoids, three cDNAs (named IeCPS1, IeCPS2 and IeCPS2a) were isolated putatively encoding copalyl diphosphate synthases from Isodon eriocalyx leaves. Recombinant proteins of IeCPS1 and IeCPS2 were expressed, respectively, in Escherichia coli, and were shown to specifically convert geranylgeranyl diphosphate to copalyl diphosphate as demonstrated by GC-MS analyses. Based on tissue-specific expression and metabolic localization studies, the IeCPS2 transcripts were detected in young and mature leaves where the dominant ent-kaurane diterpenoid maoecrystal B accumulates, whereas no detectable expression of IeCPS2 was observed in germinating seeds where the gibberellin biosynthetic pathway is usually active. In addition, no evidence for maoecrystal B was found in germinating seeds. On the other hand, IeCPS1 transcripts significantly accumulated in germinating seeds as well as in leaves. The biochemical and molecular genetic evidence thus indicated that leCPS2 is a copalyl diphosphate synthase potentially involved in the biosynthesis of Isodon diterpenoids in leaves, while IeCPS1 is more probably relevant to gibberellin formation and may, in addition, participate in Isodon ent-kaurane diterpenoid production. (C) 2012 Elsevier Ltd. All rights reserved.
关键词	Isodon Eriocalyx Lamiaceae Enzymology Labdane-related Diterpenoid Copalyl Diphosphate Synthase Isodon Ent-kauranoid Iecps2 Iecps1
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1016/j.phytochem.2011.12.021
收录类别	SCI
语种	英语
WOS记录号	WOS:000302444500003
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/10397
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China 2.Nanjing Univ, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Jiangsu, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Jun-Ling,Chen, Qian-Qian,Jin, Qiu-Ping,et al. IeCPS2 is potentially involved in the biosynthesis pharmacologically active Isodon diterpenoids rather than gibberellin[J]. PHYTOCHEMISTRY,2012,76:32-39.
APA	Li, Jun-Ling.,Chen, Qian-Qian.,Jin, Qiu-Ping.,Gao, Juan.,Zhao, Pei-Ji.,...&Zeng, Ying.(2012).IeCPS2 is potentially involved in the biosynthesis pharmacologically active Isodon diterpenoids rather than gibberellin.PHYTOCHEMISTRY,76,32-39.
MLA	Li, Jun-Ling,et al."IeCPS2 is potentially involved in the biosynthesis pharmacologically active Isodon diterpenoids rather than gibberellin".PHYTOCHEMISTRY 76(2012):32-39.

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