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题名: IeCPS2 is potentially involved in the biosynthesis pharmacologically active Isodon diterpenoids rather than gibberellin
作者: Li, Jun-Ling1, 3; Chen, Qian-Qian1, 3; Jin, Qiu-Ping2; Gao, Juan1, 3; Zhao, Pei-Ji1; Lu, Shan2; Ying ZENG(曾英)1
刊名: PHYTOCHEMISTRY
关键词: Isodon eriocalyx ; Lamiaceae ; Enzymology ; Labdane-related diterpenoid ; Copalyl diphosphate synthase ; Isodon ent-kauranoid ; IeCPS2 ; IeCPS1
英文摘要: The traditional Chinese medicinal plant, Isodon L, is remarkably rich in pharmacologically active ent-kaurane diterpenoids of diverse carbon skeletons. In an effort to create a resource for gene discovery and elucidate the biosynthesis of Isodon ent-kaurane diterpenoids, three cDNAs (named IeCPS1, IeCPS2 and IeCPS2a) were isolated putatively encoding copalyl diphosphate synthases from Isodon eriocalyx leaves. Recombinant proteins of IeCPS1 and IeCPS2 were expressed, respectively, in Escherichia coli, and were shown to specifically convert geranylgeranyl diphosphate to copalyl diphosphate as demonstrated by GC-MS analyses. Based on tissue-specific expression and metabolic localization studies, the IeCPS2 transcripts were detected in young and mature leaves where the dominant ent-kaurane diterpenoid maoecrystal B accumulates, whereas no detectable expression of IeCPS2 was observed in germinating seeds where the gibberellin biosynthetic pathway is usually active. In addition, no evidence for maoecrystal B was found in germinating seeds. On the other hand, IeCPS1 transcripts significantly accumulated in germinating seeds as well as in leaves. The biochemical and molecular genetic evidence thus indicated that leCPS2 is a copalyl diphosphate synthase potentially involved in the biosynthesis of Isodon diterpenoids in leaves, while IeCPS1 is more probably relevant to gibberellin formation and may, in addition, participate in Isodon ent-kaurane diterpenoid production. (C) 2012 Elsevier Ltd. All rights reserved.
出版日期: 2012-04-01
卷号: 76, 页码:32-39
语种: 英语
收录类别: SCI
学科分类: Biochemistry & Molecular Biology; Plant Sciences
ISSN号: 0031-9422
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/10397
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China
2.Nanjing Univ, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Jiangsu, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Li, JL; Chen, QQ; Jin, QP; Gao, J; Zhao, PJ; Lu, S; Zeng, Y.IeCPS2 is potentially involved in the biosynthesis pharmacologically active Isodon diterpenoids rather than gibberellin,PHYTOCHEMISTRY,2012,76():32-39
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