A Chitosan Induced 9-Lipoxygenase in Adelostemma gracillimum Seedlings

doi:10.3390/ijms13010540

	A Chitosan Induced 9-Lipoxygenase in Adelostemma gracillimum Seedlings
	Li, Jing1,2,3 ; Zhao, Pei-Ji 1; Ma, Chang-Le 3; Zeng, Ying1
通讯作者	Zeng, Y (reprint author), Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China, lijing@mail.kib.ac.cn ; plenty@mail.kib.ac.cn ; machangle@sina.com ; biochem@mail.kib.ac.cn
	2012
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN	1661-6596
卷号	13 期号:1 页码:540-551
摘要	Oxylipins generated by the lipoxygenase (LOX) pathway play an important role in plant defense against biotic and abiotic stress. In chitosan-treated Adelostemma gracillimum seedlings, obvious accumulation of 9-LOX-derived oxylipins, namely 9,10,11-trihydroxy-12-octadecenoic acid, was detected. Using degenerate primers, a LOX-specific fragment putatively encoding LOX was obtained by RT-PCR, and a 2.9-kb full-length cDNA named AgLOX1 was isolated by RACE from chitosan-induced A. gracillimum seedlings. Genomic Southern analysis implied that there was only one copy of AgLOX1 in the A. gracillimum genome. AgLOX1 was expressed in Escherichia coli and the recombinant protein was partially purified. The enzyme converted linoleic and linolenic acids almost exclusively to their 9-hydroperoxides. AgLOX1 encoded a 9-lipoxygenase. Northern blot analysis indicated that chitosan-induced AgLOX1 transcript accumulation peaked at 8 h after initiation of treatment, whereas trihydroxy derivatives accumulation was highest at 24 h after elicitation. Results showed that chitosan-induced AgLOX1 encoded a 9-lipoxygenase potentially involved in the defense response through 9-LOX pathway leading to biosynthesis of antimicrobial compounds in A. gracillimum seedlings.
关键词	Adelostemma Gracillimum Lipoxygenase Chitosan Trihydroxyl Octadecenoic Acid Functional Expression Aglox1
学科领域	Chemistry, Multidisciplinary
DOI	10.3390/ijms13010540
收录类别	SCI
语种	英语
WOS记录号	WOS:000300184800036
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/10387
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.SW Forestry Univ, Life Sci Coll, Kunming 650224, Peoples R China
推荐引用方式 GB/T 7714	Li, Jing,Zhao, Pei-Ji,Ma, Chang-Le,et al. A Chitosan Induced 9-Lipoxygenase in Adelostemma gracillimum Seedlings[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2012,13(1):540-551.
APA	Li, Jing,Zhao, Pei-Ji,Ma, Chang-Le,&Zeng, Ying.(2012).A Chitosan Induced 9-Lipoxygenase in Adelostemma gracillimum Seedlings.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,13(1),540-551.
MLA	Li, Jing,et al."A Chitosan Induced 9-Lipoxygenase in Adelostemma gracillimum Seedlings".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 13.1(2012):540-551.

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