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题名: A Chitosan Induced 9-Lipoxygenase in Adelostemma gracillimum Seedlings
作者: Li, Jing1, 2, 3; Zhao, Pei-Ji1; Ma, Chang-Le3; Ying ZENG(曾英)1
刊名: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
关键词: Adelostemma gracillimum ; lipoxygenase ; chitosan ; trihydroxyl octadecenoic acid ; functional expression ; AgLOX1
英文摘要: Oxylipins generated by the lipoxygenase (LOX) pathway play an important role in plant defense against biotic and abiotic stress. In chitosan-treated Adelostemma gracillimum seedlings, obvious accumulation of 9-LOX-derived oxylipins, namely 9,10,11-trihydroxy-12-octadecenoic acid, was detected. Using degenerate primers, a LOX-specific fragment putatively encoding LOX was obtained by RT-PCR, and a 2.9-kb full-length cDNA named AgLOX1 was isolated by RACE from chitosan-induced A. gracillimum seedlings. Genomic Southern analysis implied that there was only one copy of AgLOX1 in the A. gracillimum genome. AgLOX1 was expressed in Escherichia coli and the recombinant protein was partially purified. The enzyme converted linoleic and linolenic acids almost exclusively to their 9-hydroperoxides. AgLOX1 encoded a 9-lipoxygenase. Northern blot analysis indicated that chitosan-induced AgLOX1 transcript accumulation peaked at 8 h after initiation of treatment, whereas trihydroxy derivatives accumulation was highest at 24 h after elicitation. Results showed that chitosan-induced AgLOX1 encoded a 9-lipoxygenase potentially involved in the defense response through 9-LOX pathway leading to biosynthesis of antimicrobial compounds in A. gracillimum seedlings.
出版日期: 2012
卷号: 13, 期号:1, 页码:540-551
DOI标识: 10.3390/ijms13010540
语种: 英语
收录类别: SCI
学科分类: Chemistry, Multidisciplinary
ISSN号: 1661-6596
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/10387
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.SW Forestry Univ, Life Sci Coll, Kunming 650224, Peoples R China

Recommended Citation:
Li, J; Zhao, PJ; Ma, CL; Zeng, Y.A Chitosan Induced 9-Lipoxygenase in Adelostemma gracillimum Seedlings,INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2012,13(1):540-551
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