A Chitosan Induced 9-Lipoxygenase in Adelostemma gracillimum Seedlings
Li, Jing1,2,3; Zhao, Pei-Ji1; Ma, Chang-Le3; Zeng, Ying1
2012
发表期刊INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN1661-6596
卷号13期号:1页码:540-551
摘要Oxylipins generated by the lipoxygenase (LOX) pathway play an important role in plant defense against biotic and abiotic stress. In chitosan-treated Adelostemma gracillimum seedlings, obvious accumulation of 9-LOX-derived oxylipins, namely 9,10,11-trihydroxy-12-octadecenoic acid, was detected. Using degenerate primers, a LOX-specific fragment putatively encoding LOX was obtained by RT-PCR, and a 2.9-kb full-length cDNA named AgLOX1 was isolated by RACE from chitosan-induced A. gracillimum seedlings. Genomic Southern analysis implied that there was only one copy of AgLOX1 in the A. gracillimum genome. AgLOX1 was expressed in Escherichia coli and the recombinant protein was partially purified. The enzyme converted linoleic and linolenic acids almost exclusively to their 9-hydroperoxides. AgLOX1 encoded a 9-lipoxygenase. Northern blot analysis indicated that chitosan-induced AgLOX1 transcript accumulation peaked at 8 h after initiation of treatment, whereas trihydroxy derivatives accumulation was highest at 24 h after elicitation. Results showed that chitosan-induced AgLOX1 encoded a 9-lipoxygenase potentially involved in the defense response through 9-LOX pathway leading to biosynthesis of antimicrobial compounds in A. gracillimum seedlings.
关键词Adelostemma Gracillimum Lipoxygenase Chitosan Trihydroxyl Octadecenoic Acid Functional Expression Aglox1
资助信息Natural Science Foundation of Yunnan Province[2009ZC085M]; State Key Laboratory of Phytochemistry and Plant Resources in West China[P2009-ZZ02]
学科领域Chemistry, Multidisciplinary
DOI10.3390/ijms13010540
收录类别SCI
语种英语
WOS研究方向Chemistry
WOS类目Chemistry, Multidisciplinary
WOS记录号WOS:000300184800036
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/10387
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.SW Forestry Univ, Life Sci Coll, Kunming 650224, Peoples R China
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Li, Jing,Zhao, Pei-Ji,Ma, Chang-Le,et al. A Chitosan Induced 9-Lipoxygenase in Adelostemma gracillimum Seedlings[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2012,13(1):540-551.
APA Li, Jing,Zhao, Pei-Ji,Ma, Chang-Le,&Zeng, Ying.(2012).A Chitosan Induced 9-Lipoxygenase in Adelostemma gracillimum Seedlings.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,13(1),540-551.
MLA Li, Jing,et al."A Chitosan Induced 9-Lipoxygenase in Adelostemma gracillimum Seedlings".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 13.1(2012):540-551.
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