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中国科学院昆明植物研究所知识管理系统
Knowledge Management System of Kunming Institute of Botany,CAS
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GST,p < 0.001) and low levels of seed-based gene flow. C. debaoensis (Cycadaceae) is an endangered species restricted to the border of Guangxi and Yunnan province in southwest China. This species has been classified into two types: sand and karst, according to the soil matrix they grow on. We examined chloroplast sequence variation of the cpDNA sequences from 11 populations of this species. Significant population genetic differentiation was detected (GST= 0.684 and FST = 0.74160). There was marked genetic differentiation between populations in the sand and karst regions and no expansion was detected. Climate changes during glacial periods have had significant effects on the current distribution of cycads. The molecular phylogenetic data, together with the geographic distribution of the haplotypes, suggest that C. debaoensis experienced range contraction during glacial periods, and that the current populations are still confined to the original refugia in southwest China which have favorable habitats in glacial period. These results imply that small refugia were maintained in both sand and karst regions during the LGM (last glacial maximum). This species had no postglacial recolonization and only stayed in these refugia up to now. The low within-population diversity of C. debaoensis suggests that there were strong bottleneck events or founder effects within each separate region during the Quaternary climatic oscillations. Relatively high genetic and haplotype diversities were detected in the newly discovered populations, which located at intermediate locality of sand regions and had morphological variation; this is probably the consequence of the admixture of different haplotypes colonizing the area from separate sources. C. micholitzii occurs in the Annan Highlands in central Vietnam near the Laos border. C. bifida occurs in North Vietnam; its distribution extends across the border into adjacent localities in Guangxi and Yunnan in China. For the comparability between them,theywere considered as the same species C. micholitzii by many academicians. The cpDNA sequences from 11 populations showed that these very controversial species, C. micholitzii and C. bifida, is paraphyletic and should belong to the same species C. micholitzii. AMOVA analysis showed that the component of among-population within region/species (76.46%) was unexpectedly larger than the among-species/region component (14.97%), which also indicates that there is no justification for recognizing two species as C. micholitzii and C. bifida. This hypothesis was also supported by the geological data, especially the neotectonic history of the indo-china block, which started to move south since Oligocene and cause the geographic isolation of these two groups. Therefore, the most likely explanation to the phenotypic similarities between these two groups may be the retention of ancestral polymorphisms in the paraphyletic group due to incomplete lineage sorting. Furthermore, the similarities may also be ascribed to pollen-mediated gene flow among geographically proximate populations and/or phenotypic convergence under similar selection schemes in the same region. C.micholitzi had the higest genetic diversity (HT = 0.980,) and genetic differentiation (GST = 0.830, NST = 0.915) among the C. micholitzii complex. The high genetic diversity might be attributed to its long evolutionary history, highly diverse habitats. The ineffective mode of seed dispersal and dramatic neotectonic movement in the distribution range of this species could result in the high genetic differentiation. 2. Phylogeographic analysis based on nuclear ribosomal sequences, We sequenced the nrDNA ITS in all 27 populations sampled, 7 haplotypes were identified, among which C. micholitzii had 6, while C. multipinnata, C. longipetiolula and C. debaoensis shared the remaining one. Compared to chloroplast genes, nuclear genes had higher correlation between genetic and geographical distance, but lower interspecies differentiation (54.42% vs 25.24%). Phylogeographical structure of C. micholitzii and C.bifida based on ITS Variation was consistent with the morphology differentiation. This similar in nuclear gene should be ascribed to pollen-mediated gene flow among geographically proximate populations.Long-distance gene flow over the two groups was clearly interrupted, which brought on the nrDNA genetic differenciation between the geographically isolated groups, to a certain extent affected the morphological variation. 3. Interspecies relationships among Cycas micholitzii complex, We analysed chloroplast sequence variation of the atpB-rbcL and psbA-trnH intergenic spacers in 27 populations sampled of C. micholitzii complex, AMOVA analysis showed that the component of among-species/region component (59.21%). However, phylogenic analysis showed that the haplotypes of C. micholitzii complex couldn`t grouped into four clusters closely corresponding to the narrowly defined C. micholitzi, C. multipinnata, C. debaoensis and C. longipetiolula. We concluded that the conflict may result from several factors: firstly incomplete lineage sorting of C. micholitzii; secondly hybridization/introgression of sympatrically cycads, which would be supported by evidence base on nrDNA ITS sequences; thirdly intramolecular recombination in cpDNA of cycads; eventually the neotectonic movement in the distribution range of this 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Institute of Botany[KIB2017003]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Sieb&order=desc&&fq=dc.project.title_filter%3AKunming%5C+Institute%5C+of%5C+Botany%5C%5BKIB2017003%5C%5D"},{"jsname":"Medicinal plant resource is one of the most important natural resources. China is well-known in the world for its richness in medicinal plant diversity and use knowledge. Some of the plant resources are developed industrially, while the others maintain the traditional use still. However, the extended resources utilization has caused the dramatic increase in demand and the exhaustion of resources. Thus, it is become one of the most important research topics that how to use medicinal resources rationally without resource depletion and those studies need to be supported by knowledge, information and data of related disciplines. On the bassis of authoritative books, literature searches and field survey, we constructed an herbal information database as an approach for data managing. Along the theme that how medicinal resources (plant species) used in various medicinal systems (traditional Chinese medicine, folk medicine), we developed a database integrating species information, herbal knowledge and usage information of about 10,000 medicinal plants, which have satisfied the data requirement of our study and analysis. In addition, we will shear data for research and decision-making by online database service. Finally, to verify the supporting function of this database in study and decision-making, and to explore new research methods of plant resources sustainable use, we applied research methods used in social statistics, such as logistic regression analysis, discriminant analysis and correspondence analysis. In addition, statistics and the analysis of typical medical systems were developed for studies on the resource usage and its sustainability factors. The data analysis showed as following: (1) Studies about plant resources sustainable utilization needs to be supported by integrated data of related subjects.(2) Applications of traditional medicines increased steadily. There was a substantial increase of Chinese patent medicines documented in each editions of pharmacopoeia, involving about 600-800 species of medicinal plant resources. But the modernization and development of medicinal plant resources have caused the resources depletion and cultivation. And there is a trend that the caltivation supplies the rescources demand (3) Whether plant resource will be depleted or not, is affected by the distribution of plant population, the rehabilitation ability of plants, the damage of human harvesting, and the advance in herb cultivation.(4) There is a considerable proportion of an unreasonable development pattern that cultivation is promoted by resource depletion. Two main reasons caused the exhaustion: lacking investigation in the supply abilities of wild plants and legging of wild species domestication. It is unwise to follow the existing developmental pattern, which will cause more resource depletion and biodiversity damage. Finally, on the basis of our study, we have proposed some reasonable strategies to avoid irrational factors in the herbal resources exploitation: Ø Develop traditional medicines furtherly for new medicinal resources. Ø Protect the plant wild resources and develop cultivation in herbal drug explotation. Ø Establish regulatory agencies and formulate policies of herbal resources development. Ø Continue to improve the database of medicinal plants with integrated data.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Sieb&order=desc&&fq=dc.project.title_filter%3AMedicinal%5C+plant%5C+resource%5C+is%5C+one%5C+of%5C+the%5C+most%5C+important%5C+natural%5C+resources.%5C+China%5C+is%5C+well%5C-known%5C+in%5C+the%5C+world%5C+for%5C+its%5C+richness%5C+in%5C+medicinal%5C+plant%5C+diversity%5C+and%5C+use%5C+knowledge.%5C+Some%5C+of%5C+the%5C+plant%5C+resources%5C+are%5C+developed%5C+industrially%2C%5C+while%5C+the%5C+others%5C+maintain%5C+the%5C+traditional%5C+use%5C+still.%5C+However%2C%5C+the%5C+extended%5C+resources%5C+utilization%5C+has%5C+caused%5C+the%5C+dramatic%5C+increase%5C+in%5C+demand%5C+and%5C+the%5C+exhaustion%5C+of%5C+resources.%5C+Thus%2C%5C+it%5C+is%5C+become%5C+one%5C+of%5C+the%5C+most%5C+important%5C+research%5C+topics%5C+that%5C+how%5C+to%5C+use%5C+medicinal%5C+resources%5C+rationally%5C+without%5C+resource%5C+depletion%5C+and%5C+those%5C+studies%5C+need%5C+to%5C+be%5C+supported%5C+by%5C+knowledge%2C%5C+information%5C+and%5C+data%5C+of%5C+related%5C+disciplines.%5C+On%5C+the%5C+bassis%5C+of%5C+authoritative%5C+books%2C%5C+literature%5C+searches%5C+and%5C+field%5C+survey%2C%5C+we%5C+constructed%5C+an%5C+herbal%5C+information%5C+database%5C+as%5C+an%5C+approach%5C+for%5C+data%5C+managing.%5C+Along%5C+the%5C+theme%5C+that%5C+how%5C+medicinal%5C+resources%5C+%5C%28plant%5C+species%5C%29%5C+used%5C+in%5C+various%5C+medicinal%5C+systems%5C+%5C%28traditional%5C+Chinese%5C+medicine%2C%5C+folk%5C+medicine%5C%29%2C%5C+we%5C+developed%5C+a%5C+database%5C+integrating%5C+species%5C+information%2C%5C+herbal%5C+knowledge%5C+and%5C+usage%5C+information%5C+of%5C+about%5C+10%2C000%5C+medicinal%5C+plants%2C%5C+which%5C+have%5C+satisfied%5C+the%5C+data%5C+requirement%5C+of%5C+our%5C+study%5C+and%5C+analysis.%5C+In%5C+addition%2C%5C+we%5C+will%5C+shear%5C+data%5C+for%5C+research%5C+and%5C+decision%5C-making%5C+by%5C+online%5C+database%5C+service.%5C+Finally%2C%5C+to%5C+verify%5C+the%5C+supporting%5C+function%5C+of%5C+this%5C+database%5C+in%5C+study%5C+and%5C+decision%5C-making%2C%5C+and%5C+to%5C+explore%5C+new%5C+research%5C+methods%5C+of%5C+plant%5C+resources%5C+sustainable%5C+use%2C%5C+we%5C+applied%5C+research%5C+methods%5C+used%5C+in%5C+social%5C+statistics%2C%5C+such%5C+as%5C+logistic%5C+regression%5C+analysis%2C%5C+discriminant%5C+analysis%5C+and%5C+correspondence%5C+analysis.%5C+In%5C+addition%2C%5C+statistics%5C+and%5C+the%5C+analysis%5C+of%5C+typical%5C+medical%5C+systems%5C+were%5C+developed%5C+for%5C+studies%5C+on%5C+the%5C+resource%5C+usage%5C+and%5C+its%5C+sustainability%5C+factors.%5C+The%5C+data%5C+analysis%5C+showed%5C+as%5C+following%5C%3A%5C+%5C%281%5C%29%5C+Studies%5C+about%5C+plant%5C+resources%5C+sustainable%5C+utilization%5C+needs%5C+to%5C+be%5C+supported%5C+by%5C+integrated%5C+data%5C+of%5C+related%5C+subjects.%5C%282%5C%29%5C+Applications%5C+of%5C+traditional%5C+medicines%5C+increased%5C+steadily.%5C+There%5C+was%5C+a%5C+substantial%5C+increase%5C+of%5C+Chinese%5C+patent%5C+medicines%5C+documented%5C+in%5C+each%5C+editions%5C+of%5C+pharmacopoeia%2C%5C+involving%5C+about%5C+600%5C-800%5C+species%5C+of%5C+medicinal%5C+plant%5C+resources.%5C+But%5C+the%5C+modernization%5C+and%5C+development%5C+of%5C+medicinal%5C+plant%5C+resources%5C+have%5C+caused%5C+the%5C+resources%5C+depletion%5C+and%5C+cultivation.%5C+And%5C+there%5C+is%5C+a%5C+trend%5C+that%5C+the%5C+caltivation%5C+supplies%5C+the%5C+rescources%5C+demand%5C+%5C%283%5C%29%5C+Whether%5C+plant%5C+resource%5C+will%5C+be%5C+depleted%5C+or%5C+not%2C%5C+is%5C+affected%5C+by%5C+the%5C+distribution%5C+of%5C+plant%5C+population%2C%5C+the%5C+rehabilitation%5C+ability%5C+of%5C+plants%2C%5C+the%5C+damage%5C+of%5C+human%5C+harvesting%2C%5C+and%5C+the%5C+advance%5C+in%5C+herb%5C+cultivation.%5C%284%5C%29%5C+There%5C+is%5C+a%5C+considerable%5C+proportion%5C+of%5C+an%5C+unreasonable%5C+development%5C+pattern%5C+that%5C+cultivation%5C+is%5C+promoted%5C+by%5C+resource%5C+depletion.%5C+Two%5C+main%5C+reasons%5C+caused%5C+the%5C+exhaustion%5C%3A%5C+lacking%5C+investigation%5C+in%5C+the%5C+supply%5C+abilities%5C+of%5C+wild%5C+plants%5C+and%5C+legging%5C+of%5C+wild%5C+species%5C+domestication.%5C+It%5C+is%5C+unwise%5C+to%5C+follow%5C+the%5C+existing%5C+developmental%5C+pattern%2C%5C+which%5C+will%5C+cause%5C+more%5C+resource%5C+depletion%5C+and%5C+biodiversity%5C+damage.%5C+Finally%2C%5C+on%5C+the%5C+basis%5C+of%5C+our%5C+study%2C%5C+we%5C+have%5C+proposed%5C+some%5C+reasonable%5C+strategies%5C+to%5C+avoid%5C+irrational%5C+factors%5C+in%5C+the%5C+herbal%5C+resources%5C+exploitation%5C%3A%5C+%C3%98%5C+Develop%5C+traditional%5C+medicines%5C+furtherly%5C+for%5C+new%5C+medicinal%5C+resources.%5C+%C3%98%5C+Protect%5C+the%5C+plant%5C+wild%5C+resources%5C+and%5C+develop%5C+cultivation%5C+in%5C+herbal%5C+drug%5C+explotation.%5C+%C3%98%5C+Establish%5C+regulatory%5C+agencies%5C+and%5C+formulate%5C+policies%5C+of%5C+herbal%5C+resources%5C+development.%5C+%C3%98%5C+Continue%5C+to%5C+improve%5C+the%5C+database%5C+of%5C+medicinal%5C+plants%5C+with%5C+integrated%5C+data."},{"jsname":"Ministry of Science and Technology, China[2013FY112600]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Sieb&order=desc&&fq=dc.project.title_filter%3AMinistry%5C+of%5C+Science%5C+and%5C+Technology%2C%5C+China%5C%5B2013FY112600%5C%5D"},{"jsname":"Mt. Jiaozi from Yunnan is located in the eastern of the Central Yunnan Plateau, falling between the Pudu river and the Xiaojiang river, between the Jinshajiang river to the north and the Zhuanlong town of Luquan county to the south. It stands in the boundary of the Sino-Himalayan forest subkingdom and the Sino-Japan forest subkingdom, so it has a significant status, dew to the heterogeneous geographical environment and the sharp elevation drop in addition. Through field survey, specimens collection and identification and literatures consultion, we have obtained a brief list of Mt. Jiaozi, about 1517 species in 141 families and 531 genera, including varieties and subspices, among of which, there are 23 species in 7 families and 12 genera of gymnosperm. Based on statistic analysis, we get some conclusions as below. 1. As a type locality for many species, with various biodiversity and as much as endemism and some endangered plants, the flora of Mt. Jiaozi is viewed as a key area which should be paid special attention to. The flora constitution of this area has complex origins and multiple elements through the statistics. 2. The modern flora of Mt. jiaozi mainly includes the Chinese endemic elements and the Asian endemic elements, these two parts take up 81.65%. The characteristic of this flora is typically temperate. 3. Mt. Jiaozi is the north or the south distribution limitation to some important species as a floristic node. For example, species as Ephedra likiangensis, Solms-Laubachia pulcherrima, Soroseris hirsute, Paraquilegia microphylla which were used to be throught as limited in the northwestern of Yunnan had been found in this area. It indicates that, Mt. Jiaozi should become one of the foci of the flora regionalization in Yunnan, even in China. 4. In the view of the endemism of 6 families and 66 genera and 544 species in East Asian, this eara is a part of the East Asiatic Kingdom. The status of the flora of Mt. jiaozi in the East Asiatic Kingdom should be classified as Central Yunnan plateau subregion--Yunnan plateau region--Sino-Himalayan forest subkingdom.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Sieb&order=desc&&fq=dc.project.title_filter%3AMt.%5C+Jiaozi%5C+from%5C+Yunnan%5C+is%5C+located%5C+in%5C+the%5C+eastern%5C+of%5C+the%5C+Central%5C+Yunnan%5C+Plateau%2C%5C+falling%5C+between%5C+the%5C+Pudu%5C+river%5C+and%5C+the%5C+Xiaojiang%5C+river%2C%5C+between%5C+the%5C+Jinshajiang%5C+river%5C+to%5C+the%5C+north%5C+and%5C+the%5C+Zhuanlong%5C+town%5C+of%5C+Luquan%5C+county%5C+to%5C+the%5C+south.%5C+It%5C+stands%5C+in%5C+the%5C+boundary%5C+of%5C+the%5C+Sino%5C-Himalayan%5C+forest%5C+subkingdom%5C+and%5C+the%5C+Sino%5C-Japan%5C+forest%5C+subkingdom%2C%5C+so%5C+it%5C+has%5C+a%5C+significant%5C+status%2C%5C+dew%5C+to%5C+the%5C+heterogeneous%5C+geographical%5C+environment%5C+and%5C+the%5C+sharp%5C+elevation%5C+drop%5C+in%5C+addition.%5C+Through%5C+field%5C+survey%2C%5C+specimens%5C+collection%5C+and%5C+identification%5C+and%5C+literatures%5C+consultion%2C%5C+we%5C+have%5C+obtained%5C+a%5C+brief%5C+list%5C+of%5C+Mt.%5C+Jiaozi%2C%5C+about%5C+1517%5C+species%5C+in%5C+141%5C+families%5C+and%5C+531%5C+genera%2C%5C+including%5C+varieties%5C+and%5C+subspices%2C%5C+among%5C+of%5C+which%2C%5C+there%5C+are%5C+23%5C+species%5C+in%5C+7%5C+families%5C+and%5C+12%5C+genera%5C+of%5C+gymnosperm.%5C+Based%5C+on%5C+statistic%5C+analysis%2C%5C+we%5C+get%5C+some%5C+conclusions%5C+as%5C+below.%5C+1.%5C+As%5C+a%5C+type%5C+locality%5C+for%5C+many%5C+species%2C%5C+with%5C+various%5C+biodiversity%5C+and%5C+as%5C+much%5C+as%5C+endemism%5C+and%5C+some%5C+endangered%5C+plants%2C%5C+the%5C+flora%5C+of%5C+Mt.%5C+Jiaozi%5C+is%5C+viewed%5C+as%5C+a%5C+key%5C+area%5C+which%5C+should%5C+be%5C+paid%5C+special%5C+attention%5C+to.%5C+The%5C+flora%5C+constitution%5C+of%5C+this%5C+area%5C+has%5C+complex%5C+origins%5C+and%5C+multiple%5C+elements%5C+through%5C+the%5C+statistics.%5C+2.%5C+The%5C+modern%5C+flora%5C+of%5C+Mt.%5C+jiaozi%5C+mainly%5C+includes%5C+the%5C+Chinese%5C+endemic%5C+elements%5C+and%5C+the%5C+Asian%5C+endemic%5C+elements%2C%5C+these%5C+two%5C+parts%5C+take%5C+up%5C+81.65%25.%5C+The%5C+characteristic%5C+of%5C+this%5C+flora%5C+is%5C+typically%5C+temperate.%5C+3.%5C+Mt.%5C+Jiaozi%5C+is%5C+the%5C+north%5C+or%5C+the%5C+south%5C+distribution%5C+limitation%5C+to%5C+some%5C+important%5C+species%5C+as%5C+a%5C+floristic%5C+node.%5C+For%5C+example%2C%5C+species%5C+as%5C+Ephedra%5C+likiangensis%2C%5C+Solms%5C-Laubachia%5C+pulcherrima%2C%5C+Soroseris%5C+hirsute%2C%5C+Paraquilegia%5C+microphylla%5C+which%5C+were%5C+used%5C+to%5C+be%5C+throught%5C+as%5C+limited%5C+in%5C+the%5C+northwestern%5C+of%5C+Yunnan%5C+had%5C+been%5C+found%5C+in%5C+this%5C+area.%5C+It%5C+indicates%5C+that%2C%5C+Mt.%5C+Jiaozi%5C+should%5C+become%5C+one%5C+of%5C+the%5C+foci%5C+of%5C+the%5C+flora%5C+regionalization%5C+in%5C+Yunnan%2C%5C+even%5C+in%5C+China.%5C+4.%5C+In%5C+the%5C+view%5C+of%5C+the%5C+endemism%5C+of%5C+6%5C+families%5C+and%5C+66%5C+genera%5C+and%5C+544%5C+species%5C+in%5C+East%5C+Asian%2C%5C+this%5C+eara%5C+is%5C+a%5C+part%5C+of%5C+the%5C+East%5C+Asiatic%5C+Kingdom.%5C+The%5C+status%5C+of%5C+the%5C+flora%5C+of%5C+Mt.%5C+jiaozi%5C+in%5C+the%5C+East%5C+Asiatic%5C+Kingdom%5C+should%5C+be%5C+classified%5C+as%5C+Central%5C+Yunnan%5C+plateau%5C+subregion%5C-%5C-Yunnan%5C+plateau%5C+region%5C-%5C-Sino%5C-Himalayan%5C+forest%5C+subkingdom."},{"jsname":"National Key Basic Research Program of China[2014CB954100]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Sieb&order=desc&&fq=dc.project.title_filter%3ANational%5C+Key%5C+Basic%5C+Research%5C+Program%5C+of%5C+China%5C%5B2014CB954100%5C%5D"},{"jsname":"National Natural Science Foundation of China[31200182]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Sieb&order=desc&&fq=dc.project.title_filter%3ANational%5C+Natural%5C+Science%5C+Foundation%5C+of%5C+China%5C%5B31200182%5C%5D"},{"jsname":"National Natural Science Foundation of China[31370252]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Sieb&order=desc&&fq=dc.project.title_filter%3ANational%5C+Natural%5C+Science%5C+Foundation%5C+of%5C+China%5C%5B31370252%5C%5D"},{"jsname":"National Natural Science Foundation of China[41571059]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Sieb&order=desc&&fq=dc.project.title_filter%3ANational%5C+Natural%5C+Science%5C+Foundation%5C+of%5C+China%5C%5B41571059%5C%5D"},{"jsname":"National Research Council of Thailand (Mae Fah Luang University)[592010200112]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Sieb&order=desc&&fq=dc.project.title_filter%3ANational%5C+Research%5C+Council%5C+of%5C+Thailand%5C+%5C%28Mae%5C+Fah%5C+Luang%5C+University%5C%29%5C%5B592010200112%5C%5D"},{"jsname":"National Research Council of Thailand (Mae Fah Luang University)[60201000201]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Sieb&order=desc&&fq=dc.project.title_filter%3ANational%5C+Research%5C+Council%5C+of%5C+Thailand%5C+%5C%28Mae%5C+Fah%5C+Luang%5C+University%5C%29%5C%5B60201000201%5C%5D"},{"jsname":"National Science Foundation of China (NSFC)[31750110478]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Sieb&order=desc&&fq=dc.project.title_filter%3ANational%5C+Science%5C+Foundation%5C+of%5C+China%5C+%5C%28NSFC%5C%29%5C%5B31750110478%5C%5D"},{"jsname":"Sophora davidii (Franch.) Skeels is an endemic species to China, and widely distributed in the dry valleys of the Himalayan-Hengduan Mountain Systems, the Yungui Plateau, the Qinling Mountain, the Loess Plateau and other places of China. Previous studies of plant phylogeography have focused mainly on some taxa from the mountainous areas of China, relatively few studies have been conducted on plant taxa from the river valleys. The population dynamics and evolutionary history of species in such habitat remain less unknown, including the factors affecting the population genetic structure and its potential refugia in glaciation. In this study, we first determine the chromosome number, ploidy and karyotype of most populations we sampled. Then, based on sequence data from two maternally inherited cpDNA and one biparentally inherited nuclear DNA fragments, our study revealed the genetic diversity and population genetic structure of S. davidii and factors affecting them. The demographic history and potential refugia of this speices were investigated and the genetic relationship among three varieties was also clarified. The main results are summarized as follows:1. Cytogeography，The chromosome number and karyotypes of 14 S. davidii populations have been studied. The results showed that the choromosome number of all the populations is 2n = 18. The interphase nuclei and prophase chromosomes of the species were found to be of the complex chromosome type and interstitial type. The results of karyotype analysis showed that 7 of 14 materials has satellites, and the number and position of satellites differ among populations, and thus revealed a series of diversified karyotypes. With most populations being of ploidy, cytogenetical divergence within the species lied mainly in chromosome size and structure. The fact that polyploidization did not occur very often for variations in Southwest China was against viewpoint that polyploidization level in this area is higher than that of other distribution areas due to the elevation of mountains and plateau. 2. Phylogeographic analysisbased on chloroplast sequence，We sequenced two cpDNA fragments rpl32-trnL(UAG)intergenic spacer and trnH-psbA spacer in 40 populations sampled, recovering 22 chlorotypes. The average with-in population diversity (hS = 0.171) was much lower than total genetic diversity (hT = 0.857). Population differentiation was high (NST = 0.924, GST = 0.801) indicating low levels of seed-based gene flow and significant phylogeographical stucture (NST > GST, P < 0.05) were exhibited by this species. The SAMOVA revealed seven diverging groups of related chlorotypes, six of them had distinct nonoverlapping geographical ranges: one in the northeast comprising 10 populations, a second with a southeast distribution comprising 22 populations, and the remaning four groups comprising 15 populations located in the west part of the species’ range along different river valleys. The genetic clustering of populations into three regions was also supported by analysis of molecular variance, which showed that most genetic variation (82.43%) was found among these three regions. Two clusters were distinguished by both phylogenetic analysis and genealogical analysis of chlorotypes, one consisting of chlorotypes from the western region and the second consisting of those from the eastern region. Significant genetic differences between the two regions might be attributed to vicariance and restricted gene flow, and this vicariance could be explained by the physical environmental heterogeneity on each side of the Tanaka-Kaiyong Line. Following the uplift of the Tibetan Plateau, the reorganization of the major river drainages was primarily caused by river separation and capture events. These historical events could change the distribution of S. davidii from fragmented to continuous (Upper/Lower Jinshajiang and Yalongjiang/Daduhe), and from continuous to fragmented (Nujiang and Jinshajiang/Honghe). However, spatial and temporal patterns of phylogeographic divergence are strongly associated with historical disjunction rather than modern drainage connections. Moreover, the following north-south split in the eastern region and effective isolation with their genetic diversity were essentially modelled by genetic drift. The higher chlorotype richness and genetic divergence for populations in western region compared with other two regions suggests that there were multipe refugia or in situ survival of S. davidii in the Himalayan-Hengduan Mountain region. Fixation of chlorotypes in the northeastern region and near fixation in the southeastern region suggest a recent colonization of these areas. We further found that this species underwent past range expansion around 37-303 thousand years ago (kya). The southeastern populations likely experienced a demographic expansion via unidirectional gene flow along rivers, while northeastern populations underwent a more northward expansion, both from initial populations (s) (21, 22, 23) preserved on eastern refugia (Jinshajiang). This process might have been accompanied with a series of founder effects or bottlenecks making populations genetically impoverished. 3. Phylogeographic analysisbased on nuclear sequence，We sequenced the nuclear (ncpGS) region in all populations sampled, recovering 23 nuclear haplotypes. Compared to cpDNA, both NST (0.470) and GST (0.338) were relatively lower, but NST was also significantly larger than GST. 37.10% of the total variation was distributed among regions which was much lower than that shown by chlorotypes. Thus, more extensive distribution of nuclear haplotypes was exhibited across the geographical range instead of the strong population subdivision observed in chlorotypes. Similarly to the chloroplast data, we found that genetic differentiation of nDNA was positively correlated with the geographical distance, but the increase in the geographical distance between populations did not increase the genetic differentiation of nDNA as rapidly as that of cpDNA. These contrasting levels between the chloroplast and nuclear genomes of S. davidii are likely due to limited gene flow of cpDNA by seeds vs. the extensive gene flow of nDNA by wind-mediated pollen in the population history. We also determined from nuclear markers that haplotype diversity was reduced in the southeastern and northeastern regions due to the loss of rare haplotypes in western region. This reduction of gene diversity is also a signature of founder events or recent bottleneck during post-glacial colonization. However, nuclear diversity within populations remains high. This provides evidence that regionally pollen flow might be sufficiently high to blur the genetic identity of founder populations over a reasonably large spatial scale.3. Relationships among three varieties，The phylogenetic analysis identified two phylogroups of chlorotypes, corresponding to S. davidii var. davidii and var. chuansinesis. The former was distinguished by the abscence of predonminant nuclear haplotype H1 of the latter. The monophyletic group of chlorotypes in var. davidii and var. liangshanesis showed their relatively close relationship. And their genetic divergence from the third variety appears to be relative to their slight morphological difference in leaf size and the divergent environmental niche spaces they occupy. Thus, the observed differences in morphological characters between var. chuansinesis and other two varieties can be explained by the seed dispersal limitation illustrated above (as inferred by geographical separation) and by environmental heterogeneity (as inferred by precipitation or elevation) or by a combination of both. After all, the geological changes, drainage reorganization, and floristic differences following the Himalayan uplift have been suggested to affect the genetic structure of S. davidii. These results provide new insights into the phylogeographic pattern of plants in China. In addition, the unique population genetic structure found in S. davidii has provided important insights into the evolutionary history of this species. The genetic profile uncovered in this study is also critical for its conservation management. Our study has uncovered the existence of at least two ‘evolutionary significant units’ independent units within S. davidii, corresponding to var. davidii from eastern region and var. chuansinensis from western region. The conservation efforts should first focus on most western populations and on the southeastern ones exhibiting high levels of genetic diversity, while the genetically homogeneous northeastern populations located in the degraded Loess Plateau should require much greater conservation 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Research Fund (TRF)[RSA5980068]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Sieb&order=desc&&fq=dc.project.title_filter%3AThailand%5C+Research%5C+Fund%5C+%5C%28TRF%5C%29%5C%5BRSA5980068%5C%5D"},{"jsname":"The Master degree thesis studied on the chemical constituents and bioactivities of Azadirachta indica and Jatropha curcas which both have insecticidal activity. 58 compounds, including four new ones, were isolated and identified from this two species by various chromatographic processes. The cytotoxicities of some compounds obtained from Azadirachta indica were evaluated. Finally, this thesis also made a review on the chemical constituents and bioactivities from plants of genus Melia. Chapter 1 Chemical constituents and bioactivities of Azadirachta indica，From the methanol extract of branches and leaves of Azadirachta indicia, 53 compounds, including four new ones, were isolated and identified based on spectral techniques. These compounds referred to sesquiterpenoids, triterpenoids, steroids and flavonoids. Two of the new compounds were C30 triterpenoids and the other two were tetranortriterpenoids. 26 compounds were evaluated for their cytotoxicities and some were found to have potential cytotoxicities. The abundant constituent, nimbolide, showed strong cytotoxicities against all the tested cell lines. Chapter 2 Chemical constituents of Jatropha curcas，Nine known compounds were isolated from the methanol extract of branches and leaves of J. curcas. Chapter 3 Review on chemical constituents and bioactivities of genus Melia，This chapter made a review on the chemical constituents and bioactivities of genus Melia based on the literatures which were reported before 2010.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Sieb&order=desc&&fq=dc.project.title_filter%3AThe%5C+Master%5C+degree%5C+thesis%5C+studied%5C+on%5C+the%5C+chemical%5C+constituents%5C+and%5C+bioactivities%5C+of%5C+Azadirachta%5C+indica%5C+and%5C+Jatropha%5C+curcas%5C+which%5C+both%5C+have%5C+insecticidal%5C+activity.%5C+58%5C+compounds%2C%5C+including%5C+four%5C+new%5C+ones%2C%5C+were%5C+isolated%5C+and%5C+identified%5C+from%5C+this%5C+two%5C+species%5C+by%5C+various%5C+chromatographic%5C+processes.%5C+The%5C+cytotoxicities%5C+of%5C+some%5C+compounds%5C+obtained%5C+from%5C+Azadirachta%5C+indica%5C+were%5C+evaluated.%5C+Finally%2C%5C+this%5C+thesis%5C+also%5C+made%5C+a%5C+review%5C+on%5C+the%5C+chemical%5C+constituents%5C+and%5C+bioactivities%5C+from%5C+plants%5C+of%5C+genus%5C+Melia.%5C+Chapter%5C+1%5C+Chemical%5C+constituents%5C+and%5C+bioactivities%5C+of%5C+Azadirachta%5C+indica%EF%BC%8CFrom%5C+the%5C+methanol%5C+extract%5C+of%5C+branches%5C+and%5C+leaves%5C+of%5C+Azadirachta%5C+indicia%2C%5C+53%5C+compounds%2C%5C+including%5C+four%5C+new%5C+ones%2C%5C+were%5C+isolated%5C+and%5C+identified%5C+based%5C+on%5C+spectral%5C+techniques.%5C+These%5C+compounds%5C+referred%5C+to%5C+sesquiterpenoids%2C%5C+triterpenoids%2C%5C+steroids%5C+and%5C+flavonoids.%5C+Two%5C+of%5C+the%5C+new%5C+compounds%5C+were%5C+C30%5C+triterpenoids%5C+and%5C+the%5C+other%5C+two%5C+were%5C+tetranortriterpenoids.%5C+26%5C+compounds%5C+were%5C+evaluated%5C+for%5C+their%5C+cytotoxicities%5C+and%5C+some%5C+were%5C+found%5C+to%5C+have%5C+potential%5C+cytotoxicities.%5C+The%5C+abundant%5C+constituent%2C%5C+nimbolide%2C%5C+showed%5C+strong%5C+cytotoxicities%5C+against%5C+all%5C+the%5C+tested%5C+cell%5C+lines.%5C+Chapter%5C+2%5C+Chemical%5C+constituents%5C+of%5C+Jatropha%5C+curcas%EF%BC%8CNine%5C+known%5C+compounds%5C+were%5C+isolated%5C+from%5C+the%5C+methanol%5C+extract%5C+of%5C+branches%5C+and%5C+leaves%5C+of%5C+J.%5C+curcas.%5C+Chapter%5C+3%5C+Review%5C+on%5C+chemical%5C+constituents%5C+and%5C+bioactivities%5C+of%5C+genus%5C+Melia%EF%BC%8CThis%5C+chapter%5C+made%5C+a%5C+review%5C+on%5C+the%5C+chemical%5C+constituents%5C+and%5C+bioactivities%5C+of%5C+genus%5C+Melia%5C+based%5C+on%5C+the%5C+literatures%5C+which%5C+were%5C+reported%5C+before%5C+2010."},{"jsname":"lastIndexed","jscount":"2024-05-24"}],"资助项目","dc.project.title_filter")'>
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Systematics and Biogeography of Aralia L. (Araliaceae):Revision of Aralia Sects. Aralia, Humiles, Nanae, andSciadodendron
期刊论文
出版物, 3111, 卷号: 57, 期号: 0, 页码: 1-172
作者:
Jun Wen
Adobe PDF(7233Kb)
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浏览/下载：254/6
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提交时间：2017/07/24
Aralia
Aralia Sect. Aralia
Aralia Sect. Dimorphanthus
Aralia Sect. Humiles
Aralia Sect. Nanae
Aralia Sect. pentapanax
Aralia Sect. Sciadodendron
Biogeography
Araliaceae
Systematics
台湾北部福山地区亚热带雨林种子雨之研究
期刊论文
出版物, 3111, 页码: 1-95
作者:
张杨家豪
Adobe PDF(2261Kb)
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浏览/下载：126/3
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提交时间：2017/07/19
台湾北部福山地区亚热带雨林幼苗之研究
期刊论文
出版物, 3111, 页码: 1-100
作者:
吕佳陵
Adobe PDF(3786Kb)
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浏览/下载：135/2
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提交时间：2017/07/19
三尖杉属植物生物碱成分研究和活性酯碱资源评价
学位论文
: 中国科学院大学, 2022
作者:
余光兴
Adobe PDF(2195Kb)
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浏览/下载：5/0
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提交时间：2024/05/14
三尖杉属,种子,三尖杉生物碱,分离鉴定,酯碱资源评价
Cephalotaxus,Seeds,Cephalotaxine alkaloids,Isolation and identification,Ester alkaloids resources evaluation
Development of Quercus acutissima (Fagaceae) pollen tubes inside pistils during the sexual reproduction process
期刊论文
PLANTA, 2022, 卷号: 256, 期号: 1, 页码: 16
作者:
Deng, Min
;
Yao, Kaiping
;
Shi, Chengcheng
;
Shao, Wen
;
Li, Qiansheng
Adobe PDF(3817Kb)
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浏览/下载：16/3
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提交时间：2024/04/30
Delayed fertilization
Fagales
Male gametophyte-pistil interaction
Oak
Ovule
CASTANEA-CRENATA SIEB
JAPANESE CHESTNUT
DOUBLE FERTILIZATION
TRANSMITTING TISSUE
CROSS-POLLINATION
MATING SYSTEM
GROWTH
OVULES
MODE
OAK
The antidiabetic potency of Amomum tsao-ko and its active flavanols, as PTP1B selective and alpha-glucosidase dual inhibitors
期刊论文
INDUSTRIAL CROPS AND PRODUCTS, 2021, 卷号: 160, 页码: 112908
作者:
He,Xiao-Feng
;
Chen,Ji-Jun
;
Huang,Xiao-Yan
;
Hu,Jing
;
Zhang,Xu-Ke
;
Guo,Yuan-Qiang
;
Zhang,Xue-Mei
;
Geng,Chang-An
Adobe PDF(5184Kb)
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浏览/下载：83/24
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提交时间：2022/04/02
Amomum tsao-ko
Amomutsaokins A-H
Flavanol-menthane conjugates
PTP1B selective inhibitors
alpha-Glucosidase inhibitors
ANTIOXIDANT ACTIVITY
ESSENTIAL OIL
CONSTITUENTS
TANNINS
CREVOST
ACID
Plastome phylogenomics of Cephalotaxus (Cephalotaxaceae) and allied genera
期刊论文
ANNALS OF BOTANY, 2021, 卷号: 127, 期号: 5, 页码: 697-708
作者:
Ji,Yunheng
;
Liu,Changkun
;
Landis,Jacob B.
;
Deng,Min
;
Chen,Jiahui
Adobe PDF(2975Kb)
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浏览/下载：74/17
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提交时间：2022/04/02
Gymnosperm
Cephalotaxaceae
Taxaceae
phylogeny
molecular dating
taxonomic delineation
recent speciation
QUANTITATIVE RECONSTRUCTION
PHYLOGENETIC-RELATIONSHIPS
EVOLUTIONARY HISTORY
MOLECULAR PHYLOGENY
SOUTHWEST CHINA
TAXACEAE
FLORA
CHLOROPLAST
SEQUENCE
CLIMATE
滇缅泰地区巨竹属（禾本科：竹亚科）的分类修订
学位论文
, 2020
作者:
许祖昌
Adobe PDF(15131Kb)
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浏览/下载：61/0
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提交时间：2023/11/02
药食同源植物绞股蓝的风味物质及其活性研究
学位论文
, 2020
作者:
王忠泽
Adobe PDF(8458Kb)
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浏览/下载：29/0
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提交时间：2023/11/02
两种植物的次生代谢产物及米团花烷二倍半萜结构修饰研究
学位论文
, 2020
作者:
耿浩
Adobe PDF(10188Kb)
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浏览/下载：41/0
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提交时间：2023/11/02
