×
验证码:
换一张
忘记密码？
记住我
×
登录
中文版
|
English
中国科学院昆明植物研究所知识管理系统
Knowledge Management System of Kunming Institute of Botany,CAS
登录
注册
ALL
ORCID
题名
作者
学科领域
关键词
资助项目
文献类型
出处
收录类别
出版者
发表日期
存缴日期
学科门类
学习讨论厅
图片搜索
粘贴图片网址
首页
研究单元&专题
作者
文献类型
学科分类
知识图谱
新闻&公告
在结果中检索
研究单元&专题
共享文献 [70]
昆明植物所硕博研究... [32]
中国科学院东亚植物... [26]
资源植物与生物技术... [12]
植物化学与西部植物资... [3]
中国西南野生生物种质... [3]
更多...
作者
李德铢 [11]
龚洵 [9]
高连明 [9]
孙航 [8]
孙卫邦 [8]
刘杰 [5]
更多...
文献类型
期刊论文 [89]
专著 [47]
学位论文 [32]
会议录 [1]
会议论文 [1]
其他 [1]
更多...
发表日期
2021 [7]
2020 [13]
2019 [8]
2018 [8]
2017 [13]
2016 [10]
更多...
语种
英语 [70]
中文 [25]
出处
MOLECULAR ... [8]
FUNGAL DIV... [6]
JOURNAL OF... [4]
ANNALS OF ... [3]
BMC PLANT ... [3]
BOTANICAL ... [3]
更多...
资助项目
GST,p < 0.001) and low levels of seed-based gene flow. C. debaoensis (Cycadaceae) is an endangered species restricted to the border of Guangxi and Yunnan province in southwest China. This species has been classified into two types: sand and karst, according to the soil matrix they grow on. We examined chloroplast sequence variation of the cpDNA sequences from 11 populations of this species. Significant population genetic differentiation was detected (GST= 0.684 and FST = 0.74160). There was marked genetic differentiation between populations in the sand and karst regions and no expansion was detected. Climate changes during glacial periods have had significant effects on the current distribution of cycads. The molecular phylogenetic data, together with the geographic distribution of the haplotypes, suggest that C. debaoensis experienced range contraction during glacial periods, and that the current populations are still confined to the original refugia in southwest China which have favorable habitats in glacial period. These results imply that small refugia were maintained in both sand and karst regions during the LGM (last glacial maximum). This species had no postglacial recolonization and only stayed in these refugia up to now. The low within-population diversity of C. debaoensis suggests that there were strong bottleneck events or founder effects within each separate region during the Quaternary climatic oscillations. Relatively high genetic and haplotype diversities were detected in the newly discovered populations, which located at intermediate locality of sand regions and had morphological variation; this is probably the consequence of the admixture of different haplotypes colonizing the area from separate sources. C. micholitzii occurs in the Annan Highlands in central Vietnam near the Laos border. C. bifida occurs in North Vietnam; its distribution extends across the border into adjacent localities in Guangxi and Yunnan in China. For the comparability between them,theywere considered as the same species C. micholitzii by many academicians. The cpDNA sequences from 11 populations showed that these very controversial species, C. micholitzii and C. bifida, is paraphyletic and should belong to the same species C. micholitzii. AMOVA analysis showed that the component of among-population within region/species (76.46%) was unexpectedly larger than the among-species/region component (14.97%), which also indicates that there is no justification for recognizing two species as C. micholitzii and C. bifida. This hypothesis was also supported by the geological data, especially the neotectonic history of the indo-china block, which started to move south since Oligocene and cause the geographic isolation of these two groups. Therefore, the most likely explanation to the phenotypic similarities between these two groups may be the retention of ancestral polymorphisms in the paraphyletic group due to incomplete lineage sorting. Furthermore, the similarities may also be ascribed to pollen-mediated gene flow among geographically proximate populations and/or phenotypic convergence under similar selection schemes in the same region. C.micholitzi had the higest genetic diversity (HT = 0.980,) and genetic differentiation (GST = 0.830, NST = 0.915) among the C. micholitzii complex. The high genetic diversity might be attributed to its long evolutionary history, highly diverse habitats. The ineffective mode of seed dispersal and dramatic neotectonic movement in the distribution range of this species could result in the high genetic differentiation. 2. Phylogeographic analysis based on nuclear ribosomal sequences, We sequenced the nrDNA ITS in all 27 populations sampled, 7 haplotypes were identified, among which C. micholitzii had 6, while C. multipinnata, C. longipetiolula and C. debaoensis shared the remaining one. Compared to chloroplast genes, nuclear genes had higher correlation between genetic and geographical distance, but lower interspecies differentiation (54.42% vs 25.24%). Phylogeographical structure of C. micholitzii and C.bifida based on ITS Variation was consistent with the morphology differentiation. This similar in nuclear gene should be ascribed to pollen-mediated gene flow among geographically proximate populations.Long-distance gene flow over the two groups was clearly interrupted, which brought on the nrDNA genetic differenciation between the geographically isolated groups, to a certain extent affected the morphological variation. 3. Interspecies relationships among Cycas micholitzii complex, We analysed chloroplast sequence variation of the atpB-rbcL and psbA-trnH intergenic spacers in 27 populations sampled of C. micholitzii complex, AMOVA analysis showed that the component of among-species/region component (59.21%). However, phylogenic analysis showed that the haplotypes of C. micholitzii complex couldn`t grouped into four clusters closely corresponding to the narrowly defined C. micholitzi, C. multipinnata, C. debaoensis and C. longipetiolula. We concluded that the conflict may result from several factors: firstly incomplete lineage sorting of C. micholitzii; secondly hybridization/introgression of sympatrically cycads, which would be supported by evidence base on nrDNA ITS sequences; thirdly intramolecular recombination in cpDNA of cycads; eventually the neotectonic movement in the distribution range of this species.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=NARROW%2BHYBRID%2BZONE&order=desc&&fq=dc.project.title_filter%3ACycas%5C+micholitzii%5C+complex%5C+is%5C+composed%5C+of%5C+5%5C+species%5C%3A%5C+C.%5C+micholitzii%5C+Dyer%2C%5C+C.%5C+bifida%5C+%5C%28Dyer%5C%29%5C+K.%5C+D.%5C+Hill%2CC.%5C+longipetiolula%5C+D.%5C+Y.%5C+Wang%2C%5C+C.%5C+debaoensis%5C+Y.%5C+C.%5C+Zhong%5C+et%5C+C%5C+J.%5C+Chen%2C%5C+C.%5C+multipinnata%5C+C%5C+J.%5C+Chen%5C+et%5C+S.%5C+Y.%5C+Yang%EF%BC%8Cand%5C+distributed%5C+from%5C+southwest%5C+China%5C+to%5C+central%5C+Vietnam%5C+and%5C+eastern%5C+Laos.%5C+Based%5C+on%5C+sequence%5C+data%5C+from%5C+two%5C+maternally%5C+inherited%5C+cpDNA%5C+and%5C+one%5C+biparentally%5C+nuclear%5C+DNA%5C+fragments%2C%5C+our%5C+study%5C+revealed%5C+the%5C+population%5C+genetic%5C+structure%5C+of%5C+C.%5C+micholitzii%5C+complex%5C+and%5C+explored%5C+the%5C+potential%5C+causes.%5C+The%5C+evolutionary%5C+and%5C+demographic%5C+histories%5C+were%5C+investigated.%5C+The%5C+genetic%5C+relationship%5C+among%5C+species%5C+in%5C+the%5C+complex%5C+was%5C+also%5C+clarified.The%5C+results%5C+were%5C+summarized%5C+as%5C+follows%5C%3A%5C+1.%5C+Phylogeographic%5C+analysis%5C+based%5C+on%5C+chloroplast%5C+sequences%EF%BC%8CWe%5C+examined%5C+chloroplast%5C+sequence%5C+variation%5C+of%5C+the%5C+atpB%5C-rbcLand%5C+psbA%5C-trnHintergenic%5C+spacers%5C+in%5C+27%5C+populations%5C+of%5C+C.%5C+micholitzii%5C+complex%2C%5C+recovering%5C+26%5C+haplotypes.%5C+The%5C+average%5C+within%5C-population%5C+diversity%5C+%5C%28HS%5C+%3D%5C+0.140%5C%29%5C+was%5C+low%5C+while%5C+total%5C+diversity%5C+%5C%28HT%5C+%3D%5C+0.911%5C%29%5C+was%5C+high.%5C+Population%5C+differentiation%5C+was%5C+also%5C+high%5C%28GST%5C+%3D%5C+0.846%2C%5C+NST%5C+%3D%5C+0.919%5C%29%2C%5C+indicating%5C+significant%5C+phylogeographical%5C+structure%5C+%5C%28NST%5C+%3E%5C+GST%2Cp%5C+%3C%5C+0.001%5C%29%5C+and%5C+low%5C+levels%5C+of%5C+seed%5C-based%5C+gene%5C+flow.%5C+C.%5C+debaoensis%5C+%5C%28Cycadaceae%5C%29%5C+is%5C+an%5C+endangered%5C+species%5C+restricted%5C+to%5C+the%5C+border%5C+of%5C+Guangxi%5C+and%5C+Yunnan%5C+province%5C+in%5C+southwest%5C+China.%5C+This%5C+species%5C+has%5C+been%5C+classified%5C+into%5C+two%5C+types%5C%3A%5C+sand%5C+and%5C+karst%2C%5C+according%5C+to%5C+the%5C+soil%5C+matrix%5C+they%5C+grow%5C+on.%5C+We%5C+examined%5C+chloroplast%5C+sequence%5C+variation%5C+of%5C+the%5C+cpDNA%5C+sequences%5C+from%5C+11%5C+populations%5C+of%5C+this%5C+species.%5C+Significant%5C+population%5C+genetic%5C+differentiation%5C+was%5C+detected%5C+%5C%28GST%3D%5C+0.684%5C+and%5C+FST%5C+%3D%5C+0.74160%5C%29.%5C+There%5C+was%5C+marked%5C+genetic%5C+differentiation%5C+between%5C+populations%5C+in%5C+the%5C+sand%5C+and%5C+karst%5C+regions%5C+and%5C+no%5C+expansion%5C+was%5C+detected.%5C+Climate%5C+changes%5C+during%5C+glacial%5C+periods%5C+have%5C+had%5C+significant%5C+effects%5C+on%5C+the%5C+current%5C+distribution%5C+of%5C+cycads.%5C+The%5C+molecular%5C+phylogenetic%5C+data%2C%5C+together%5C+with%5C+the%5C+geographic%5C+distribution%5C+of%5C+the%5C+haplotypes%2C%5C+suggest%5C+that%5C+C.%5C+debaoensis%5C+experienced%5C+range%5C+contraction%5C+during%5C+glacial%5C+periods%2C%5C+and%5C+that%5C+the%5C+current%5C+populations%5C+are%5C+still%5C+confined%5C+to%5C+the%5C+original%5C+refugia%5C+in%5C+southwest%5C+China%5C+which%5C+have%5C+favorable%5C+habitats%5C+in%5C+glacial%5C+period.%5C+These%5C+results%5C+imply%5C+that%5C+small%5C+refugia%5C+were%5C+maintained%5C+in%5C+both%5C+sand%5C+and%5C+karst%5C+regions%5C+during%5C+the%5C+LGM%5C+%5C%28last%5C+glacial%5C+maximum%5C%29.%5C+This%5C+species%5C+had%5C+no%5C+postglacial%5C+recolonization%5C+and%5C+only%5C+stayed%5C+in%5C+these%5C+refugia%5C+up%5C+to%5C+now.%5C+The%5C+low%5C+within%5C-population%5C+diversity%5C+of%5C+C.%5C+debaoensis%5C+suggests%5C+that%5C+there%5C+were%5C+strong%5C+bottleneck%5C+events%5C+or%5C+founder%5C+effects%5C+within%5C+each%5C+separate%5C+region%5C+during%5C+the%5C+Quaternary%5C+climatic%5C+oscillations.%5C+Relatively%5C+high%5C+genetic%5C+and%5C+haplotype%5C+diversities%5C+were%5C+detected%5C+in%5C+the%5C+newly%5C+discovered%5C+populations%2C%5C+which%5C+located%5C+at%5C+intermediate%5C+locality%5C+of%5C+sand%5C+regions%5C+and%5C+had%5C+morphological%5C+variation%5C%3B%5C+this%5C+is%5C+probably%5C+the%5C+consequence%5C+of%5C+the%5C+admixture%5C+of%5C+different%5C+haplotypes%5C+colonizing%5C+the%5C+area%5C+from%5C+separate%5C+sources.%5C+%5C+C.%5C+micholitzii%5C+occurs%5C+in%5C+the%5C+Annan%5C+Highlands%5C+in%5C+central%5C+Vietnam%5C+near%5C+the%5C+Laos%5C+border.%5C+C.%5C+bifida%5C+occurs%5C+in%5C+North%5C+Vietnam%5C%3B%5C+its%5C+distribution%5C+extends%5C+across%5C+the%5C+border%5C+into%5C+adjacent%5C+localities%5C+in%5C+Guangxi%5C+and%5C+Yunnan%5C+in%5C+China.%5C+For%5C+the%5C+comparability%5C+between%5C+them%2Ctheywere%5C+considered%5C+as%5C+the%5C+same%5C+species%5C+C.%5C+micholitzii%5C+by%5C+many%5C+academicians.%5C+The%5C+cpDNA%5C+sequences%5C+from%5C+11%5C+populations%5C+showed%5C+that%5C+these%5C+very%5C+controversial%5C+species%2C%5C+C.%5C+micholitzii%5C+and%5C+C.%5C+bifida%2C%5C+is%5C+paraphyletic%5C+and%5C+should%5C+belong%5C+to%5C+the%5C+same%5C+species%5C+C.%5C+micholitzii.%5C+AMOVA%5C+analysis%5C+showed%5C+that%5C+the%5C+component%5C+of%5C+among%5C-population%5C+within%5C+region%5C%2Fspecies%5C+%5C%2876.46%25%5C%29%5C+was%5C+unexpectedly%5C+larger%5C+than%5C+the%5C+among%5C-species%5C%2Fregion%5C+component%5C+%5C%2814.97%25%5C%29%2C%5C+which%5C+also%5C+indicates%5C+that%5C+there%5C+is%5C+no%5C+justification%5C+for%5C+recognizing%5C+two%5C+species%5C+as%5C+C.%5C+micholitzii%5C+and%5C+C.%5C+bifida.%5C+This%5C+hypothesis%5C+was%5C+also%5C+supported%5C+by%5C+the%5C+geological%5C+data%2C%5C+especially%5C+the%5C+neotectonic%5C+history%5C+of%5C+the%5C+indo%5C-china%5C+block%2C%5C+which%5C+started%5C+to%5C+move%5C+south%5C+since%5C+Oligocene%5C+and%5C+cause%5C+the%5C+geographic%5C+isolation%5C+of%5C+these%5C+two%5C+groups.%5C+Therefore%2C%5C+the%5C+most%5C+likely%5C+explanation%5C+to%5C+the%5C+phenotypic%5C+similarities%5C+between%5C+these%5C+two%5C+groups%5C+may%5C+be%5C+the%5C+retention%5C+of%5C+ancestral%5C+polymorphisms%5C+in%5C+the%5C+paraphyletic%5C+group%5C+due%5C+to%5C+incomplete%5C+lineage%5C+sorting.%5C+Furthermore%2C%5C+the%5C+similarities%5C+may%5C+also%5C+be%5C+ascribed%5C+to%5C+pollen%5C-mediated%5C+gene%5C+flow%5C+among%5C+geographically%5C+proximate%5C+populations%5C+and%5C%2For%5C+phenotypic%5C+convergence%5C+under%5C+similar%5C+selection%5C+schemes%5C+in%5C+the%5C+same%5C+region.%5C+C.micholitzi%5C+had%5C+the%5C+higest%5C+genetic%5C+diversity%5C+%5C%28HT%5C+%3D%5C+0.980%2C%5C%29%5C+and%5C+genetic%5C+differentiation%5C+%5C%28GST%5C+%3D%5C+0.830%2C%5C+NST%5C+%3D%5C+0.915%5C%29%5C+among%5C+the%5C+C.%5C+micholitzii%5C+complex.%5C+The%5C+high%5C+genetic%5C+diversity%5C+might%5C+be%5C+attributed%5C+to%5C+its%5C+long%5C+evolutionary%5C+history%2C%5C+highly%5C+diverse%5C+habitats.%5C+The%5C+ineffective%5C+mode%5C+of%5C+seed%5C+dispersal%5C+and%5C+dramatic%5C+neotectonic%5C+movement%5C+in%5C+the%5C+distribution%5C+range%5C+of%5C+this%5C+species%5C+could%5C+result%5C+in%5C+the%5C+high%5C+genetic%5C+differentiation.%5C+2.%5C+Phylogeographic%5C+analysis%5C+based%5C+on%5C+nuclear%5C+ribosomal%5C+sequences%2C%5C+We%5C+sequenced%5C+the%5C+nrDNA%5C+ITS%5C+in%5C+all%5C+27%5C+populations%5C+sampled%2C%5C+7%5C+haplotypes%5C+were%5C+identified%2C%5C+among%5C+which%5C+C.%5C+micholitzii%5C+had%5C+6%2C%5C+while%5C+C.%5C+multipinnata%2C%5C+C.%5C+longipetiolula%5C+and%5C+C.%5C+debaoensis%5C+shared%5C+the%5C+remaining%5C+one.%5C+Compared%5C+to%5C+chloroplast%5C+genes%2C%5C+nuclear%5C+genes%5C+had%5C+higher%5C+correlation%5C+between%5C+genetic%5C+and%5C+geographical%5C+distance%2C%5C+but%5C+lower%5C+interspecies%5C+differentiation%5C+%5C%2854.42%25%5C+vs%5C+25.24%25%5C%29.%5C+Phylogeographical%5C+structure%5C+of%5C+C.%5C+micholitzii%5C+and%5C+C.bifida%5C+based%5C+on%5C+ITS%5C+Variation%5C+was%5C+consistent%5C+with%5C+the%5C+morphology%5C+differentiation.%5C+This%5C+similar%5C+in%5C+nuclear%5C+gene%5C+should%5C+be%5C+ascribed%5C+to%5C+pollen%5C-mediated%5C+gene%5C+flow%5C+among%5C+geographically%5C+proximate%5C+populations.Long%5C-distance%5C+gene%5C+flow%5C+over%5C+the%5C+two%5C+groups%5C+was%5C+clearly%5C+interrupted%2C%5C+which%5C+brought%5C+on%5C+the%5C+nrDNA%5C+genetic%5C+differenciation%5C+between%5C+the%5C+geographically%5C+isolated%5C+groups%2C%5C+to%5C+a%5C+certain%5C+extent%5C+affected%5C+the%5C+morphological%5C+variation.%5C+3.%5C+Interspecies%5C+relationships%5C+among%5C+Cycas%5C+micholitzii%5C+complex%2C%5C+We%5C+analysed%5C+chloroplast%5C+sequence%5C+variation%5C+of%5C+the%5C+atpB%5C-rbcL%5C+and%5C+psbA%5C-trnH%5C+intergenic%5C+spacers%5C+in%5C+27%5C+populations%5C+sampled%5C+of%5C+C.%5C+micholitzii%5C+complex%2C%5C+AMOVA%5C+analysis%5C+showed%5C+that%5C+the%5C+component%5C+of%5C+among%5C-species%5C%2Fregion%5C+component%5C+%5C%2859.21%25%5C%29.%5C+However%2C%5C+phylogenic%5C+analysis%5C+showed%5C+that%5C+the%5C+haplotypes%5C+of%5C+C.%5C+micholitzii%5C+complex%5C+couldn%60t%5C+grouped%5C+into%5C+four%5C+clusters%5C+closely%5C+corresponding%5C+to%5C+the%5C+narrowly%5C+defined%5C+C.%5C+micholitzi%2C%5C+C.%5C+multipinnata%2C%5C+C.%5C+debaoensis%5C+and%5C+C.%5C+longipetiolula.%5C+We%5C+concluded%5C+that%5C+the%5C+conflict%5C+may%5C+result%5C+from%5C+several%5C+factors%5C%3A%5C+firstly%5C+incomplete%5C+lineage%5C+sorting%5C+of%5C+C.%5C+micholitzii%5C%3B%5C+secondly%5C+hybridization%5C%2Fintrogression%5C+of%5C+sympatrically%5C+cycads%2C%5C+which%5C+would%5C+be%5C+supported%5C+by%5C+evidence%5C+base%5C+on%5C+nrDNA%5C+ITS%5C+sequences%5C%3B%5C+thirdly%5C+intramolecular%5C+recombination%5C+in%5C+cpDNA%5C+of%5C+cycads%5C%3B%5C+eventually%5C+the%5C+neotectonic%5C+movement%5C+in%5C+the%5C+distribution%5C+range%5C+of%5C+this%5C+species."},{"jsname":"Kunming Institute of Botany[KIB2017003]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=NARROW%2BHYBRID%2BZONE&order=desc&&fq=dc.project.title_filter%3AKunming%5C+Institute%5C+of%5C+Botany%5C%5BKIB2017003%5C%5D"},{"jsname":"Ministry of Science and Technology, China[2013FY112600]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=NARROW%2BHYBRID%2BZONE&order=desc&&fq=dc.project.title_filter%3AMinistry%5C+of%5C+Science%5C+and%5C+Technology%2C%5C+China%5C%5B2013FY112600%5C%5D"},{"jsname":"National Key Basic Research Program of China[2014CB954100]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=NARROW%2BHYBRID%2BZONE&order=desc&&fq=dc.project.title_filter%3ANational%5C+Key%5C+Basic%5C+Research%5C+Program%5C+of%5C+China%5C%5B2014CB954100%5C%5D"},{"jsname":"National Natural Science Foundation of China[31200182]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=NARROW%2BHYBRID%2BZONE&order=desc&&fq=dc.project.title_filter%3ANational%5C+Natural%5C+Science%5C+Foundation%5C+of%5C+China%5C%5B31200182%5C%5D"},{"jsname":"National Natural Science Foundation of China[31370252]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=NARROW%2BHYBRID%2BZONE&order=desc&&fq=dc.project.title_filter%3ANational%5C+Natural%5C+Science%5C+Foundation%5C+of%5C+China%5C%5B31370252%5C%5D"},{"jsname":"National Natural Science Foundation of China[41571059]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=NARROW%2BHYBRID%2BZONE&order=desc&&fq=dc.project.title_filter%3ANational%5C+Natural%5C+Science%5C+Foundation%5C+of%5C+China%5C%5B41571059%5C%5D"},{"jsname":"National Research Council of Thailand (Mae Fah Luang University)[592010200112]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=NARROW%2BHYBRID%2BZONE&order=desc&&fq=dc.project.title_filter%3ANational%5C+Research%5C+Council%5C+of%5C+Thailand%5C+%5C%28Mae%5C+Fah%5C+Luang%5C+University%5C%29%5C%5B592010200112%5C%5D"},{"jsname":"National Research Council of Thailand (Mae Fah Luang University)[60201000201]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=NARROW%2BHYBRID%2BZONE&order=desc&&fq=dc.project.title_filter%3ANational%5C+Research%5C+Council%5C+of%5C+Thailand%5C+%5C%28Mae%5C+Fah%5C+Luang%5C+University%5C%29%5C%5B60201000201%5C%5D"},{"jsname":"National Science Foundation of China (NSFC)[31750110478]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=NARROW%2BHYBRID%2BZONE&order=desc&&fq=dc.project.title_filter%3ANational%5C+Science%5C+Foundation%5C+of%5C+China%5C+%5C%28NSFC%5C%29%5C%5B31750110478%5C%5D"},{"jsname":"National Science Foundation of China[31470336]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=NARROW%2BHYBRID%2BZONE&order=desc&&fq=dc.project.title_filter%3ANational%5C+Science%5C+Foundation%5C+of%5C+China%5C%5B31470336%5C%5D"},{"jsname":"National Science Foundation of China[31600178]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=NARROW%2BHYBRID%2BZONE&order=desc&&fq=dc.project.title_filter%3ANational%5C+Science%5C+Foundation%5C+of%5C+China%5C%5B31600178%5C%5D"},{"jsname":"Natural hybridization has many evolutionary consequences, such as the origin of new ecotypes or species and the maintenance of reproductive barriers. However, few studies have involved in and quantified the potential threat to rare species by natural hybridization. In this study, we focused on the hybridization between a narrow endemic species R. cyanocarpum Franch. ex W.W. Sm and the widespread species R. delavayi Franch.. We revealed this process of natural hybridization, composition and possible significance of the detected single hybrid zone and comparative analysis of fitness among parental species and the hybrids. These significant results were summarized as follows: 1. Field observation and comparative analysis of morphological characters, We investigated 6 sites where R. cyanocarpum distributed, and found the single putative hybrid zone only in one site. 22 morphological characters were selected and measured, according to which the putative parental species were determined. Among the examined morphological characters, intermediate characters accounted for 45%, whereas the transgressive characters accounted for 14%. These results showed that intermediate characters were predominant. In addition, the ecological adaptations of these transgressive characters remain unknown. 2. Reproductive biology and reproductive evidence for natural hybridization, Three main results were achieved via the study of pollination biology of R. cyanocarpum: (1) from the results of floral color experiments, we found that the pink flowers have preponderant adaptation over the white ones. Because either proportions of individuals with pink flowers within and between populations, or visitation rate of pollinators were all significant higher than white types; (2) the effective pollinators of R. cyanocarpum were solely the bumblebees (Bombus festivces and B. richardsi), and the pollination is primarily due to geitonogamy that is mediated by bumblebees; (3) most of fruits were either composed of 5 or 6 carpels. Moreover, the proportions of carpel number per fruit were significantly related to the altitudes ((r=0.807, p<0.01) and the positions of inflorescence (r=0.62, p<0.01). Meanwhile, seed weight, seed number and seed germination rate were significant difference to the fruits with different carpel number; For R. delavayi, the experiment showed that the associated function of nectary and spots promote reproductive success in R. delavayi, because of significantly higher fruit production was examined in half petal deprived with degenerative nectarines than in half petal deprived with normal ones. Reciprocal hand pollination treatments showed that either species, as pollen donor or pollen receiver, could produce fruits indicating weak reproductive barriers between species. It was noted that fruit set varied among treatments. The same pollinators (bumblebees) were shared in both parental species. Therefore, flowering period overlapping, weak reproductive barriers and sharing the same pollinators, all contributed to reproductive evidence for the possible occurrence of natural hybridization between R. cyanocarpum and R. delavayi. 3. Molecular evidence for natural hybridization, On the basis of two nuclear DNA (ITS and Waxy) and three chloroplast DNA (trnC-F, trnH-psbA and trnL-rpl32), hybrids and parental species were clearly identified and individuals with intermediate morphologies are indeed of hybrid origin from natural hybridization between R. cyanocarpum and R. delavayi. Hybridization occurred in both directions, but was asymmetrical, with R. delavayi as the major maternal parent (90%) whereas only one hybrid had identical chloroplast to R. cyanocarpum (10%). 4. The study of hybrid zone, Two molecular methods were employed to successfully disclosed characters of the single hybrid zone: (1) this hybrid zone was composed of parental species, F1s, F2s and backcross derivatives; (2) F2s outnumbered either F1s or backcross derivatives; (3) the direction of gene flow was from R. cyanocarpum to R. delavayi. Based on the one directional gene flow and fewer hybrids formation in the single hybrid zone, no threat to R. cyanocarpum posed by natural hybridization was observed. However, such a threat is likely to arise if more hybrids are produced via serious habitat disturbance. 5. Comparative analysis of fitness among parental species and hybrids, We made a preliminary result that the fitness of hybrids’ offspring is not always lower than their parental offsprings, after comparative analysis of some detected fitness index in experimental condition, such as the lengths of radicle and hypocotyls, and the seedling height. Therefore, it is reasonable to presume that these hybrids can have similar or higher fitness than parental species only if natural habitat is changed substantially in a short period.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=NARROW%2BHYBRID%2BZONE&order=desc&&fq=dc.project.title_filter%3ANatural%5C+hybridization%5C+has%5C+many%5C+evolutionary%5C+consequences%2C%5C+such%5C+as%5C+the%5C+origin%5C+of%5C+new%5C+ecotypes%5C+or%5C+species%5C+and%5C+the%5C+maintenance%5C+of%5C+reproductive%5C+barriers.%5C+However%2C%5C+few%5C+studies%5C+have%5C+involved%5C+in%5C+and%5C+quanti%EF%AC%81ed%5C+the%5C+potential%5C+threat%5C+to%5C+rare%5C+species%5C+by%5C+natural%5C+hybridization.%5C+In%5C+this%5C+study%2C%5C+we%5C+focused%5C+on%5C+the%5C+hybridization%5C+between%5C+a%5C+narrow%5C+endemic%5C+species%5C+R.%5C+cyanocarpum%5C+Franch.%5C+ex%5C+W.W.%5C+Sm%5C+and%5C+the%5C+widespread%5C+species%5C+R.%5C+delavayi%5C+Franch..%5C+We%5C+revealed%5C+this%5C+process%5C+of%5C+natural%5C+hybridization%2C%5C+composition%5C+and%5C+possible%5C+significance%5C+of%5C+the%5C+detected%5C+single%5C+hybrid%5C+zone%5C+and%5C+comparative%5C+analysis%5C+of%5C+fitness%5C+among%5C+parental%5C+species%5C+and%5C+the%5C+hybrids.%5C+These%5C+significant%5C+results%5C+were%5C+summarized%5C+as%5C+follows%5C%3A%5C+1.%5C+Field%5C+observation%5C+and%5C+comparative%5C+analysis%5C+of%5C+morphological%5C+characters%2C%5C+We%5C+investigated%5C+6%5C+sites%5C+where%5C+R.%5C+cyanocarpum%5C+distributed%2C%5C+and%5C+found%5C+the%5C+single%5C+putative%5C+hybrid%5C+zone%5C+only%5C+in%5C+one%5C+site.%5C+22%5C+morphological%5C+characters%5C+were%5C+selected%5C+and%5C+measured%2C%5C+according%5C+to%5C+which%5C+the%5C+putative%5C+parental%5C+species%5C+were%5C+determined.%5C+Among%5C+the%5C+examined%5C+morphological%5C+characters%2C%5C+intermediate%5C+characters%5C+accounted%5C+for%5C+45%25%2C%5C+whereas%5C+the%5C+transgressive%5C+characters%5C+accounted%5C+for%5C+14%25.%5C+These%5C+results%5C+showed%5C+that%5C+intermediate%5C+characters%5C+were%5C+predominant.%5C+In%5C+addition%2C%5C+the%5C+ecological%5C+adaptations%5C+of%5C+these%5C+transgressive%5C+characters%5C+remain%5C+unknown.%5C+2.%5C+Reproductive%5C+biology%5C+and%5C+reproductive%5C+evidence%5C+for%5C+natural%5C+hybridization%2C%5C+Three%5C+main%5C+results%5C+were%5C+achieved%5C+via%5C+the%5C+study%5C+of%5C+pollination%5C+biology%5C+of%5C+R.%5C+cyanocarpum%5C%3A%5C+%5C%281%5C%29%5C+from%5C+the%5C+results%5C+of%5C+floral%5C+color%5C+experiments%2C%5C+we%5C+found%5C+that%5C+the%5C+pink%5C+flowers%5C+have%5C+preponderant%5C+adaptation%5C+over%5C+the%5C+white%5C+ones.%5C+Because%5C+either%5C+proportions%5C+of%5C+individuals%5C+with%5C+pink%5C+flowers%5C+within%5C+and%5C+between%5C+populations%2C%5C+or%5C+visitation%5C+rate%5C+of%5C+pollinators%5C+were%5C+all%5C+significant%5C+higher%5C+than%5C+white%5C+types%5C%3B%5C+%5C%282%5C%29%5C+the%5C+effective%5C+pollinators%5C+of%5C+R.%5C+cyanocarpum%5C+were%5C+solely%5C+the%5C+bumblebees%5C+%5C%28Bombus%5C+festivces%5C+and%5C+B.%5C+richardsi%5C%29%2C%5C+and%5C+the%5C+pollination%5C+is%5C+primarily%5C+due%5C+to%5C+geitonogamy%5C+that%5C+is%5C+mediated%5C+by%5C+bumblebees%5C%3B%5C+%5C%283%5C%29%5C+most%5C+of%5C+fruits%5C+were%5C+either%5C+composed%5C+of%5C+5%5C+or%5C+6%5C+carpels.%5C+Moreover%2C%5C+the%5C+proportions%5C+of%5C+carpel%5C+number%5C+per%5C+fruit%5C+were%5C+significantly%5C+related%5C+to%5C+the%5C+altitudes%5C+%5C%28%5C%28r%3D0.807%2C%5C+p%3C0.01%5C%29%5C+and%5C+the%5C+positions%5C+of%5C+inflorescence%5C+%5C%28r%3D0.62%2C%5C+p%3C0.01%5C%29.%5C+Meanwhile%2C%5C+seed%5C+weight%2C%5C+seed%5C+number%5C+and%5C+seed%5C+germination%5C+rate%5C+were%5C+significant%5C+difference%5C+to%5C+the%5C+fruits%5C+with%5C+different%5C+carpel%5C+number%5C%3B%C2%A0For%5C+R.%5C+delavayi%2C%5C+the%5C+experiment%5C+showed%5C+that%5C+the%5C+associated%5C+function%5C+of%5C+nectary%5C+and%5C+spots%5C+promote%5C+reproductive%5C+success%5C+in%5C+R.%5C+delavayi%2C%5C+because%5C+of%5C+significantly%5C+higher%5C+fruit%5C+production%5C+was%5C+examined%5C+in%5C+half%5C+petal%5C+deprived%5C+with%5C+degenerative%5C+nectarines%5C+than%5C+in%5C+half%5C+petal%5C+deprived%5C+with%5C+normal%5C+ones.%5C+Reciprocal%5C+hand%5C+pollination%5C+treatments%5C+showed%5C+that%5C+either%5C+species%2C%5C+as%5C+pollen%5C+donor%5C+or%5C+pollen%5C+receiver%2C%5C+could%5C+produce%5C+fruits%5C+indicating%5C+weak%5C+reproductive%5C+barriers%5C+between%5C+species.%5C+It%5C+was%5C+noted%5C+that%5C+fruit%5C+set%5C+varied%5C+among%5C+treatments.%5C+The%5C+same%5C+pollinators%5C+%5C%28bumblebees%5C%29%5C+were%5C+shared%5C+in%5C+both%5C+parental%5C+species.%5C+Therefore%2C%5C+flowering%5C+period%5C+overlapping%2C%5C+weak%5C+reproductive%5C+barriers%5C+and%5C+sharing%5C+the%5C+same%5C+pollinators%2C%5C+all%5C+contributed%5C+to%5C+reproductive%5C+evidence%5C+for%5C+the%5C+possible%5C+occurrence%5C+of%5C+natural%5C+hybridization%5C+between%5C+R.%5C+cyanocarpum%5C+and%5C+R.%5C+delavayi.%5C+3.%5C+Molecular%5C+evidence%5C+for%5C+natural%5C+hybridization%2C%5C+On%5C+the%5C+basis%5C+of%5C+two%5C+nuclear%5C+DNA%5C+%5C%28ITS%5C+and%5C+Waxy%5C%29%5C+and%5C+three%5C+chloroplast%5C+DNA%5C+%5C%28trnC%5C-F%2C%5C+trnH%5C-psbA%5C+and%5C+trnL%5C-rpl32%5C%29%2C%5C+hybrids%5C+and%5C+parental%5C+species%5C+were%5C+clearly%5C+identi%EF%AC%81ed%5C+and%5C+individuals%5C+with%5C+intermediate%5C+morphologies%5C+are%5C+indeed%5C+of%5C+hybrid%5C+origin%5C+from%5C+natural%5C+hybridization%5C+between%5C+R.%5C+cyanocarpum%5C+and%5C+R.%5C+delavayi.%5C+Hybridization%5C+occurred%5C+in%5C+both%5C+directions%2C%5C+but%5C+was%5C+asymmetrical%2C%5C+with%5C+R.%5C+delavayi%5C+as%5C+the%5C+major%5C+maternal%5C+parent%5C+%5C%2890%25%5C%29%5C+whereas%5C+only%5C+one%5C+hybrid%5C+had%5C+identical%5C+chloroplast%5C+to%5C+R.%5C+cyanocarpum%5C+%5C%2810%25%5C%29.%5C+4.%5C+The%5C+study%5C+of%5C+hybrid%5C+zone%2C%5C+Two%5C+molecular%5C+methods%5C+were%5C+employed%5C+to%5C+successfully%5C+disclosed%5C+characters%5C+of%5C+the%5C+single%5C+hybrid%5C+zone%5C%3A%5C+%5C%281%5C%29%5C+this%5C+hybrid%5C+zone%5C+was%5C+composed%5C+of%5C+parental%5C+species%2C%5C+F1s%2C%5C+F2s%5C+and%5C+backcross%5C+derivatives%5C%3B%5C+%5C%282%5C%29%5C+F2s%5C+outnumbered%5C+either%5C+F1s%5C+or%5C+backcross%5C+derivatives%5C%3B%5C+%5C%283%5C%29%5C+the%5C+direction%5C+of%5C+gene%5C+flow%5C+was%5C+from%5C+R.%5C+cyanocarpum%5C+to%5C+R.%5C+delavayi.%5C+Based%5C+on%5C+the%5C+one%5C+directional%5C+gene%5C+flow%5C+and%5C+fewer%5C+hybrids%5C+formation%5C+in%5C+the%5C+single%5C+hybrid%5C+zone%2C%5C+no%5C+threat%5C+to%5C+R.%5C+cyanocarpum%5C+posed%5C+by%5C+natural%5C+hybridization%5C+was%5C+observed.%5C+However%2C%5C+such%5C+a%5C+threat%5C+is%5C+likely%5C+to%5C+arise%5C+if%5C+more%5C+hybrids%5C+are%5C+produced%5C+via%5C+serious%5C+habitat%5C+disturbance.%5C+5.%5C+Comparative%5C+analysis%5C+of%5C+fitness%5C+among%5C+parental%5C+species%5C+and%5C+hybrids%2C%5C+We%5C+made%5C+a%5C+preliminary%5C+result%5C+that%5C+the%5C+fitness%5C+of%5C+hybrids%E2%80%99%5C+offspring%5C+is%5C+not%5C+always%5C+lower%5C+than%5C+their%5C+parental%5C+offsprings%2C%5C+after%5C+comparative%5C+analysis%5C+of%5C+some%5C+detected%5C+fitness%5C+index%5C+in%5C+experimental%5C+condition%2C%5C+such%5C+as%5C+the%5C+lengths%5C+of%5C+radicle%5C+and%5C+hypocotyls%2C%5C+and%5C+the%5C+seedling%5C+height.%5C+Therefore%2C%5C+it%5C+is%5C+reasonable%5C+to%5C+presume%5C+that%5C+these%5C+hybrids%5C+can%5C+have%5C+similar%5C+or%5C+higher%5C+fitness%5C+than%5C+parental%5C+species%5C+only%5C+if%5C+natural%5C+habitat%5C+is%5C+changed%5C+substantially%5C+in%5C+a%5C+short%5C+period."},{"jsname":"Polyploidy appears to be the most significant mode of sympatric speciation, it is a prevalent phenomenon in species. In this research to investigate the distribution pattern we analysed 27 populations over 500 individuals’ karyotypes and compared morphological characters between different populations to investigate the morphological polymorphism of different ploidy. Parsimony analysis within 2 non-coding loci from chloroplast genome and rDNA ITS, combined the karyotype and morphological results to disscussed the way and the frequency of tetraploids origin. The major results are shown as follows:1. Karyotypes of different populations in A. mairei，27 populations were studied, 13 diploid and 14 tetraploid populations have been detected. No populations have both ploidy coexist. Three karyotype formulas in diploid: 2n=16m，2n=14m+2sm and 2n=12m+4sm; Four karyotype formulas in tetraploid: 2n=30m+2sm，2n=28m+4sm，2n=26m+6sm，2n=28m+2sm+2st. The karyotypes of all populations showed great similarity. Most chromosomes types are “m” and“sm”,only one “st”found in Songming population. The As.K.% ranged from 54.70% to 57.96% .All karyotype found showed original with type 1A or 2A. Based on the evolutionary trend of karyotype is symmetry to asymmetry, A. mairei is very original in karyotype even compared with other species in the genus Allium.2 .The distribution pattern of populations with different ploidy，Both cytotypes occur across a wide range in the distribution area. But the diploid cytotype was rarely found in the east of Yunnan province, and diploid cytotype prefer higher altitude. The tetraploid is extremely common in the east of Yunnan but rarely found in the west, and tetraploid prefer lower altitudes. No correlation found between the distribution patterns and the karyotype asymmetry. Bisides few populations, the karyotype asymmetry in most tetraploid population are similar with diploid, and the smallest karyotype asymmetry found in tetraploid. So based on the karyotype couldn’t found the evolution trend between two poloidy in A. maimei. This result may implied the multiple origins of the tetraploid in A. maimei, some progenitors of tetraploid were distinct, caused no correlation found between two ploidy. Another possibility is the tetraploid spread very quickly after origins, so the change of the karyotype is not obvious. 3. Morphological Polymorphism of different ploidy，The analysis of variation and multiple comparisons with a result of all characters significantly different among /in populations.Characters using numerical taxonomic method Q cluster reveals that ploidy level has little correlation with the morphological variation. The morphological variations in A. mairei are more correlate with Climte and environment. Because the diploids in west Yunnan have more morphological variations. No obvious morphological differentiation between diploid and tetraploid，the tetraploids in A. maimei are autopolyploid from the sight of morphology.4. Autopolyploid and multiple origins of tetraploids in A. mairei，The parsimony analyses of ITS sequence based on the sampling strategy of A. mairei populations with different species of Allium. The strict consensus tree show the diploids and tetraploids of A. mairei to be monophyletic (100% bootstrap). No hybridization between A. mairei and relatives. The result indicates independent polyploidization processes in A. mairei, and support tetraploids in A. mairei are autopolyploid. Based on 27 haplotypes derived form two cpDNA fragments (rpl32-trnL and psbD-trhT), the network analyses showed tetraploids in A.mairei have arisen at least three times. Phylogenetic analyses based on ITS variation types showed the reproductive isolation between diploid and teraploid may not exist.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=NARROW%2BHYBRID%2BZONE&order=desc&&fq=dc.project.title_filter%3APolyploidy%5C+appears%5C+to%5C+be%5C+the%5C+most%5C+significant%5C+mode%5C+of%5C+sympatric%5C+speciation%2C%5C+it%5C+is%5C+a%5C+prevalent%5C+phenomenon%5C+in%5C+species.%5C+In%5C+this%5C+research%5C+to%5C+investigate%5C+the%5C+distribution%5C+pattern%5C+we%5C+analysed%5C+27%5C+populations%5C+over%5C+500%5C+individuals%E2%80%99%5C+karyotypes%5C+and%5C+compared%5C+morphological%5C+characters%5C+between%5C+different%5C+populations%5C+to%5C+investigate%5C+the%5C+morphological%5C+polymorphism%5C+of%5C+different%5C+ploidy.%5C+Parsimony%5C+analysis%5C+within%5C+2%5C+non%5C-coding%5C+loci%5C+from%5C+chloroplast%5C+genome%5C+and%5C+rDNA%5C+ITS%2C%5C+combined%5C+the%5C+karyotype%5C+and%5C+morphological%5C+results%5C+to%5C+disscussed%5C+the%5C+way%5C+and%5C+the%5C+frequency%5C+of%5C+tetraploids%5C+origin.%5C+The%5C+major%5C+results%5C+are%5C+shown%5C+as%5C+follows%5C%3A1.%5C+Karyotypes%5C+of%5C+different%5C+populations%5C+in%5C+A.%5C+mairei%EF%BC%8C27%5C+populations%5C+were%5C+studied%2C%5C+13%5C+diploid%5C+and%5C+14%5C+tetraploid%5C+populations%5C+have%5C+been%5C+detected.%5C+No%5C+populations%5C+have%5C+both%5C+ploidy%5C+coexist.%5C+Three%5C+karyotype%5C+formulas%5C+in%5C+diploid%5C%3A%5C+2n%3D16m%EF%BC%8C2n%3D14m%5C%2B2sm%5C+and%5C+2n%3D12m%5C%2B4sm%5C%3B%5C+Four%5C+karyotype%5C+formulas%5C+in%5C+tetraploid%5C%3A%5C+2n%3D30m%5C%2B2sm%EF%BC%8C2n%3D28m%5C%2B4sm%EF%BC%8C2n%3D26m%5C%2B6sm%EF%BC%8C2n%3D28m%5C%2B2sm%5C%2B2st.%5C+The%5C+karyotypes%5C+of%5C+all%5C+populations%5C+showed%5C+great%5C+similarity.%5C+Most%5C+chromosomes%5C+types%5C+are%5C+%E2%80%9Cm%E2%80%9D%5C+and%E2%80%9Csm%E2%80%9D%2Conly%5C+one%5C+%E2%80%9Cst%E2%80%9Dfound%5C+in%5C+Songming%5C+population.%5C+The%5C+As.K.%25%5C+ranged%5C+from%5C+54.70%25%5C+to%5C+57.96%25%5C+.All%5C+karyotype%5C+found%5C+showed%5C+original%5C+with%5C+type%5C+1A%5C+or%5C+2A.%5C+Based%5C+on%5C+the%5C+evolutionary%5C+trend%5C+of%5C+karyotype%5C+is%5C+symmetry%5C+to%5C+asymmetry%2C%5C+A.%5C+mairei%5C+is%5C+very%5C+original%5C+in%5C+karyotype%5C+even%5C+compared%5C+with%5C+other%5C+species%5C+in%5C+the%5C+genus%5C+Allium.2%5C+.The%5C+distribution%5C+pattern%5C+of%5C+populations%5C+with%5C+different%5C+ploidy%EF%BC%8CBoth%5C+cytotypes%5C+occur%5C+across%5C+a%5C+wide%5C+range%5C+in%5C+the%5C+distribution%5C+area.%5C+But%5C+the%5C+diploid%5C+cytotype%5C+was%5C+rarely%5C+found%5C+in%5C+the%5C+east%5C+of%5C+Yunnan%5C+province%2C%5C+and%5C+diploid%5C+cytotype%5C+prefer%5C+higher%5C+altitude.%5C+The%5C+tetraploid%5C+is%5C+extremely%5C+common%5C+in%5C+the%5C+east%5C+of%5C+Yunnan%5C+but%5C+rarely%5C+found%5C+in%5C+the%5C+west%2C%5C+and%5C+tetraploid%5C+prefer%5C+lower%5C+altitudes.%5C+No%5C+correlation%5C+found%5C+between%5C+the%5C+distribution%5C+patterns%5C+and%5C+the%5C+karyotype%5C+asymmetry.%5C+Bisides%5C+few%5C+populations%2C%5C+the%5C+karyotype%5C+asymmetry%5C+in%5C+most%5C+tetraploid%5C+population%5C+are%5C+similar%5C+with%5C+diploid%2C%5C+and%5C+the%5C+smallest%5C+karyotype%5C+asymmetry%5C+found%5C+in%5C+tetraploid.%5C+So%5C+based%5C+on%5C+the%5C+karyotype%5C+couldn%E2%80%99t%5C+found%5C+the%5C+evolution%5C+trend%5C+between%5C+two%5C+poloidy%5C+in%5C+A.%5C+maimei.%5C+This%5C+result%5C+may%5C+implied%5C+the%5C+multiple%5C+origins%5C+of%5C+the%5C+tetraploid%5C+in%5C+A.%5C+maimei%2C%5C+some%5C+progenitors%5C+of%5C+tetraploid%5C+were%5C+distinct%2C%5C+caused%5C+no%5C+correlation%5C+found%5C+between%5C+two%5C+ploidy.%5C+Another%5C+possibility%5C+is%5C+the%5C+tetraploid%5C+spread%5C+very%5C+quickly%5C+after%5C+origins%2C%5C+so%5C+the%5C+change%5C+of%5C+the%5C+karyotype%5C+is%5C+not%5C+obvious.%5C+3.%5C+Morphological%5C+Polymorphism%5C+of%5C+different%5C+ploidy%EF%BC%8CThe%5C+analysis%5C+of%5C+variation%5C+and%5C+multiple%5C+comparisons%5C+with%5C+a%5C+result%5C+of%5C+all%5C+characters%5C+significantly%5C+different%5C+among%5C+%5C%2Fin%5C+populations.Characters%5C+using%5C+numerical%5C+taxonomic%5C+method%5C+Q%5C+cluster%5C+reveals%5C+that%5C+ploidy%5C+level%5C+has%5C+little%5C+correlation%5C+with%5C+the%5C+morphological%5C+variation.%5C+The%5C+morphological%5C+variations%5C+in%5C+A.%5C+mairei%5C+are%5C+more%5C+correlate%5C+with%5C+Climte%5C+and%5C+environment.%5C+Because%5C+the%5C+diploids%5C+in%5C+west%5C+Yunnan%5C+have%5C+more%5C+morphological%5C+variations.%5C+No%5C+obvious%5C+morphological%5C+differentiation%5C+between%5C+diploid%5C+and%5C+tetraploid%EF%BC%8Cthe%5C+tetraploids%5C+in%5C+A.%5C+maimei%5C+are%5C+autopolyploid%5C+from%5C+the%5C+sight%5C+of%5C+morphology.4.%5C+Autopolyploid%5C+and%5C+multiple%5C+origins%5C+of%5C+tetraploids%5C+in%5C+A.%5C+mairei%EF%BC%8CThe%5C+parsimony%5C+analyses%5C+of%5C+ITS%5C+sequence%5C+based%5C+on%5C+the%5C+sampling%5C+strategy%5C+of%5C+A.%5C+mairei%5C+populations%5C+with%5C+different%5C+species%5C+of%5C+Allium.%5C+The%5C+strict%5C+consensus%5C+tree%5C+show%5C+the%5C+diploids%5C+and%5C+tetraploids%5C+of%5C+A.%5C+mairei%5C+to%5C+be%5C+monophyletic%5C+%5C%28100%25%5C+bootstrap%5C%29.%5C+No%5C+hybridization%5C+between%5C+A.%5C+mairei%5C+and%5C+relatives.%5C+The%5C+result%5C+indicates%5C+independent%5C+polyploidization%5C+processes%5C+in%5C+A.%5C+mairei%2C%5C+and%5C+support%5C+tetraploids%5C+in%5C+A.%5C+mairei%5C+are%5C+autopolyploid.%5C+Based%5C+on%5C+27%5C+haplotypes%5C+derived%5C+form%5C+two%5C+cpDNA%5C+fragments%5C+%5C%28rpl32%5C-trnL%5C+and%5C+psbD%5C-trhT%5C%29%2C%5C+the%5C+network%5C+analyses%5C+showed%5C+tetraploids%5C+in%5C+A.mairei%5C+have%5C+arisen%5C+at%5C+least%5C+three%5C+times.%5C+Phylogenetic%5C+analyses%5C+based%5C+on%5C+ITS%5C+variation%5C+types%5C+showed%5C+the%5C+reproductive%5C+isolation%5C+between%5C+diploid%5C+and%5C+teraploid%5C+may%5C+not%5C+exist."},{"jsname":"Polyploidy, the presence of three or more genomes in an organism, has occurred extensively in plants, and plays a major role in the evolution and speciation of angiosperm. Despite extensive study of the cytotypes distribution and origin of polyploidy, few studies have been reported in China, especially in southwest region. Allium wallichii Kunth (Alliaceae) is a perennial herb, distributed in southwest China, northen India, Nepal, Sikkim and Bhutan. The multiple ploidy levels and rapid differentiation has made A. wallichii a good candidate for studying polyploidy. The cytotypes distribution and origin of A. wallichii polyploidy has been studied in Yunnan-Guizhou Plateu, a main distribution area of this plant. Root-tip squashes were used to identify the ploidy level and karyotypes of 412 plants from 17 populations sampled from Yunnan-Guizhou Plateau. Based on nuclear ITS DNA sequences in 83 individuals from 17 populations, phylogentic analysis were performed to investigate types of A. wallichii polyloids, and determine if the reproductive isolation has been established between diploids and tetraploids. Based on two chloroplast DNA (cpDNA) fragments (petL-psbE, trnQ-rps16), haplotypes were identified , and the origination of tetraploids were analyzed. The main results and conclusions are as follows: 1. Distribution of cytotypes, Cytotype distribution was analyzed based on 412 newly studied plants from seventeen populations and published literatures. Nine diploid populations, six diploid-tetraploid mixed populations occur in central to northwest Yunnan, while twelve tetraploid populations occur in the Yunnan-Guizhou Plateau. Furthermore, tetraploids has a wider altitude range (1400-3726 m) than diploids (2100-3638 m), which suggests the adaptative ability of tetraploid is much stronger than its diploid pregnancies. Tetraploid populations distributed in northwest Yunnan have much lower karyotype asymmetry with the asymmetry indexes (AI) ranges from 1.83 to 2.87 compared to other populations of other areas (AI, 2.03-3.02). This suggests tetraploid in northwest Yunnan is likely to have an earlier derivation. 2. Autoploidization origin of the tetraploids, Diploids are all 2A type except that Zhongdian D and Baoshan populations are 3A type, and tetraploids are all 2A type but Huize and Hezhang populations are 2B type. It shows high similarity of karyotypes of diploid and tetraploids. In addition, the undistinguishable morphology of A. wallichii with different ploidy levels in northwest Yunnan and the monophyly of A. wallichii in ITS strict consensus tree all suggest autopolyploid origins of tetraploids A. wallichii. 3. Multiple origins of tetraploids, Based on two cpDNA fragments (petL-psbE, trnQ-rps16) in 85 individuals from 17 populations across the Yunnan-Guizhou Plateau, a total of 17 haplotypes were identified, among them, 3 in diploids only, 11 in tetraploids only, and 3 found in both cytotypes. This, plus network analyses, indicated that tetraploids have arisen independently from diploids at least three times. 4. Productive isolation between diploids and teraploids, ITS phylogenetic analyses between diploid and tetraploid A. wallichii shows that diploids and tetraploids are both monophyly, with bootstrap value 100% and 88% respectively, indicating that the reproductive isolation has been established between them. Based on cpDNA haplotypes and ITS variation types analyses, extensive hybridization and gene introgression may have occurred among tetraploids.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=NARROW%2BHYBRID%2BZONE&order=desc&&fq=dc.project.title_filter%3APolyploidy%2C%5C+the%5C+presence%5C+of%5C+three%5C+or%5C+more%5C+genomes%5C+in%5C+an%5C+organism%2C%5C+has%5C+occurred%5C+extensively%5C+in%5C+plants%2C%5C+and%5C+plays%5C+a%5C+major%5C+role%5C+in%5C+the%5C+evolution%5C+and%5C+speciation%5C+of%5C+angiosperm.%5C+Despite%5C+extensive%5C+study%5C+of%5C+the%5C+cytotypes%5C+distribution%5C+and%5C+origin%5C+of%5C+polyploidy%2C%5C+few%5C+studies%5C+have%5C+been%5C+reported%5C+in%5C+China%2C%5C+especially%5C+in%5C+southwest%5C+region.%5C+Allium%5C+wallichii%5C+Kunth%5C+%5C%28Alliaceae%5C%29%5C+is%5C+a%5C+perennial%5C+herb%2C%5C+distributed%5C+in%5C+southwest%5C+China%2C%5C+northen%5C+India%2C%5C+Nepal%2C%5C+Sikkim%5C+and%5C+Bhutan.%5C+The%5C+multiple%5C+ploidy%5C+levels%5C+and%5C+rapid%5C+differentiation%5C+has%5C+made%5C+A.%5C+wallichii%5C+a%5C+good%5C+candidate%5C+for%5C+studying%5C+polyploidy.%5C+The%5C+cytotypes%5C+distribution%5C+and%5C+origin%5C+of%5C+A.%5C+wallichii%5C+polyploidy%5C+has%5C+been%5C+studied%5C+in%5C+Yunnan%5C-Guizhou%5C+Plateu%2C%5C+a%5C+main%5C+distribution%5C+area%5C+of%5C+this%5C+plant.%5C+Root%5C-tip%5C+squashes%5C+were%5C+used%5C+to%5C+identify%5C+the%5C+ploidy%5C+level%5C+and%5C+karyotypes%5C+of%5C+412%5C+plants%5C+from%5C+17%5C+populations%5C+sampled%5C+from%5C+Yunnan%5C-Guizhou%5C+Plateau.%5C+Based%5C+on%5C+nuclear%5C+ITS%5C+DNA%5C+sequences%5C+in%5C+83%5C+individuals%5C+from%5C+17%5C+populations%2C%5C+phylogentic%5C+analysis%5C+were%5C+performed%5C+to%5C+investigate%5C+types%5C+of%5C+A.%5C+wallichii%5C+polyloids%2C%5C+and%5C+determine%5C+if%5C+the%5C+reproductive%5C+isolation%5C+has%5C+been%5C+established%5C+between%5C+diploids%5C+and%5C+tetraploids.%5C+Based%5C+on%5C+two%5C+chloroplast%5C+DNA%5C+%5C%28cpDNA%5C%29%5C+fragments%5C+%5C%28petL%5C-psbE%2C%5C+trnQ%5C-rps16%5C%29%2C%5C+haplotypes%5C+were%5C+identified%5C+%2C%5C+and%5C+the%5C+origination%5C+of%5C+tetraploids%5C+were%5C+analyzed.%5C+The%5C+main%5C+results%5C+and%5C+conclusions%5C+are%5C+as%5C+follows%5C%3A%5C+1.%5C+Distribution%5C+of%5C+cytotypes%2C%5C+Cytotype%5C+distribution%5C+was%5C+analyzed%5C+based%5C+on%5C+412%5C+newly%5C+studied%5C+plants%5C+from%5C+seventeen%5C+populations%5C+and%5C+published%5C+literatures.%5C+Nine%5C+diploid%5C+populations%2C%5C+six%5C+diploid%5C-tetraploid%5C+mixed%5C+populations%5C+occur%5C+in%5C+central%5C+to%5C+northwest%5C+Yunnan%2C%5C+while%5C+twelve%5C+tetraploid%5C+populations%5C+occur%5C+in%5C+the%5C+Yunnan%5C-Guizhou%5C+Plateau.%5C+Furthermore%2C%5C+tetraploids%5C+has%5C+a%5C+wider%5C+altitude%5C+range%5C+%5C%281400%5C-3726%5C+m%5C%29%5C+than%5C+diploids%5C+%5C%282100%5C-3638%5C+m%5C%29%2C%5C+which%5C+suggests%5C+the%5C+adaptative%5C+ability%5C+of%5C+tetraploid%5C+is%5C+much%5C+stronger%5C+than%5C+its%5C+diploid%5C+pregnancies.%5C+Tetraploid%5C+populations%5C+distributed%5C+in%5C+northwest%5C+Yunnan%5C+have%5C+much%5C+lower%5C+karyotype%5C+asymmetry%5C+with%5C+the%5C+asymmetry%5C+indexes%5C+%5C%28AI%5C%29%5C+ranges%5C+from%5C+1.83%5C+to%5C+2.87%5C+compared%5C+to%5C+other%5C+populations%5C+of%5C+other%5C+areas%5C+%5C%28AI%2C%5C+2.03%5C-3.02%5C%29.%5C+This%5C+suggests%5C+tetraploid%5C+in%5C+northwest%5C+Yunnan%5C+is%5C+likely%5C+to%5C+have%5C+an%5C+earlier%5C+derivation.%5C+2.%5C+Autoploidization%5C+origin%5C+of%5C+the%5C+tetraploids%2C%5C+Diploids%5C+are%5C+all%5C+2A%5C+type%5C+except%5C+that%5C+Zhongdian%5C+D%5C+and%5C+Baoshan%5C+populations%5C+are%5C+3A%5C+type%2C%5C+and%5C+tetraploids%5C+are%5C+all%5C+2A%5C+type%5C+but%5C+Huize%5C+and%5C+Hezhang%5C+populations%5C+are%5C+2B%5C+type.%5C+It%5C+shows%5C+high%5C+similarity%5C+of%5C+karyotypes%5C+of%5C+diploid%5C+and%5C+tetraploids.%5C+In%5C+addition%2C%5C+the%5C+undistinguishable%5C+morphology%5C+of%5C+A.%5C+wallichii%5C+with%5C+different%5C+ploidy%5C+levels%5C+in%5C+northwest%5C+Yunnan%5C+and%5C+the%5C+monophyly%5C+of%5C+A.%5C+wallichii%5C+in%5C+ITS%5C+strict%5C+consensus%5C+tree%5C+all%5C+suggest%5C+autopolyploid%5C+origins%5C+of%5C+tetraploids%5C+A.%5C+wallichii.%5C+3.%5C+Multiple%5C+origins%5C+of%5C+tetraploids%2C%5C+Based%5C+on%5C+two%5C+cpDNA%5C+fragments%5C+%5C%28petL%5C-psbE%2C%5C+trnQ%5C-rps16%5C%29%5C+in%5C+85%5C+individuals%5C+from%5C+17%5C+populations%5C+across%5C+the%5C+Yunnan%5C-Guizhou%5C+Plateau%2C%5C+a%5C+total%5C+of%5C+17%5C+haplotypes%5C+were%5C+identified%2C%5C+among%5C+them%2C%5C+3%5C+in%5C+diploids%5C+only%2C%5C+11%5C+in%5C+tetraploids%5C+only%2C%5C+and%5C+3%5C+found%5C+in%5C+both%5C+cytotypes.%5C+This%2C%5C+plus%5C+network%5C+analyses%2C%5C+indicated%5C+that%5C+tetraploids%5C+have%5C+arisen%5C+independently%5C+from%5C+diploids%5C+at%5C+least%5C+three%5C+times.%5C+4.%5C+Productive%5C+isolation%5C+between%5C+diploids%5C+and%5C+teraploids%2C%5C+ITS%5C+phylogenetic%5C+analyses%5C+between%5C+diploid%5C+and%5C+tetraploid%5C+A.%5C+wallichii%5C+shows%5C+that%5C+diploids%5C+and%5C+tetraploids%5C+are%5C+both%5C+monophyly%2C%5C+with%5C+bootstrap%5C+value%5C+100%25%5C+and%5C+88%25%5C+respectively%2C%5C+indicating%5C+that%5C+the%5C+reproductive%5C+isolation%5C+has%5C+been%5C+established%5C+between%5C+them.%5C+Based%5C+on%5C+cpDNA%5C+haplotypes%5C+and%5C+ITS%5C+variation%5C+types%5C+analyses%2C%5C+extensive%5C+hybridization%5C+and%5C+gene%5C+introgression%5C+may%5C+have%5C+occurred%5C+among%5C+tetraploids."},{"jsname":"Thailand Research Fund (TRF)[RSA5980068]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=NARROW%2BHYBRID%2BZONE&order=desc&&fq=dc.project.title_filter%3AThailand%5C+Research%5C+Fund%5C+%5C%28TRF%5C%29%5C%5BRSA5980068%5C%5D"},{"jsname":"lastIndexed","jscount":"2024-05-24"}],"资助项目","dc.project.title_filter")'>
Astilbe Bu... [1]
CAS Presid... [1]
China Scho... [1]
Chinese Ac... [1]
Cycas mich... [1]
Kunming In... [1]
更多...
收录类别
SCI [49]
资助机构
John D. an... [2]
2560A30702... [1]
31200183 [1]
31270434) [1]
31460012) [1]
31460015 [1]
更多...
×
知识图谱
KIB OpenIR
开始提交
已提交作品
待认领作品
已认领作品
未提交全文
收藏管理
QQ客服
官方微博
反馈留言
浏览/检索结果:
共171条，第1-10条
帮助
已选(
0
)
清除
条数/页：
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
排序方式：
请选择
作者升序
作者降序
WOS被引频次升序
WOS被引频次降序
期刊影响因子升序
期刊影响因子降序
发表日期升序
发表日期降序
题名升序
题名降序
提交时间升序
提交时间降序
Systematics and Biogeography of Aralia L. (Araliaceae):Revision of Aralia Sects. Aralia, Humiles, Nanae, andSciadodendron
期刊论文
出版物, 3111, 卷号: 57, 期号: 0, 页码: 1-172
作者:
Jun Wen
Adobe PDF(7233Kb)
|
收藏
|
浏览/下载：254/6
|
提交时间：2017/07/24
Aralia
Aralia Sect. Aralia
Aralia Sect. Dimorphanthus
Aralia Sect. Humiles
Aralia Sect. Nanae
Aralia Sect. pentapanax
Aralia Sect. Sciadodendron
Biogeography
Araliaceae
Systematics
HANDBOOKOF BIOLOGICAL STATISTICS
期刊论文
出版物, 3111, 期号: 0, 页码: 1-291
作者:
JOHN H. MCDONALD
Adobe PDF(1363Kb)
|
收藏
|
浏览/下载：244/2
|
提交时间：2017/07/24
Evolutionary ecology of plant-plant interactions
期刊论文
出版物, 3111, 页码: 1-144
作者:
Zuo Z(作者)
Adobe PDF(717Kb)
|
收藏
|
浏览/下载：232/4
|
提交时间：2017/07/19
Appressorial interactions with host and their evolution
期刊论文
FUNGAL DIVERSITY, 2021, 卷号: 110, 期号: 1, 页码: 75-107
作者:
Chethana,K. W. Thilini
;
Jayawardena,Ruvishika S.
;
Chen,Yi-Jyun
;
Konta,Sirinapa
;
Tibpromma,Saowaluck
;
Phukhamsakda,Chayanard
;
Abeywickrama,Pranami D.
;
Samarakoon,Milan C.
;
Senwanna,Chanokned
;
Mapook,Ausana
;
Tang,Xia
;
Gomdola,Deecksha
;
Marasinghe,Diana S.
;
Padaruth,Oundhyalah D.
;
Balasuriya,Abhaya
;
Xu,Jianping
;
Lumyong,Saisamorn
;
Hyde,Kevin D.
Adobe PDF(8605Kb)
|
收藏
|
浏览/下载：78/12
|
提交时间：2022/04/02
Ancestral characters
Evolution
Host-recognition
Hyaline appressoria
Infection process
Melanized appressoria
Proto-appressoria
ACTIVATED PROTEIN-KINASE
UROMYCES-VICIAE-FABAE
INFECTION STRUCTURE FORMATION
SCANNING-ELECTRON-MICROSCOPY
BEAUVERIA-BASSIANA INFECTION
BOTRYTIS-CINEREA VIRULENCE
BIOLOGICAL-CONTROL AGENTS
WALL-DEGRADING ENZYMES
GREY MOLD FUNGUS
ENTOMOPATHOGENIC FUNGUS
Spatiotemporal maintenance of flora in the Himalaya biodiversity hotspot: Current knowledge and future perspectives
期刊论文
ECOLOGY AND EVOLUTION, 2021, 卷号: 11, 期号: 16, 页码: 10794-10812
作者:
Wambulwa,Moses C.
;
Milne,Richard
;
Wu,Zeng-Yuan
;
Spicer,Robert A.
;
Provan,Jim
;
Luo,Ya-Huang
;
Zhu,Guang-Fu
;
Wang,Wan-Ting
;
Wang,Hong
;
Gao,Lian-Ming
;
Li,De-Zhu
;
Liu,Jie
Adobe PDF(2089Kb)
|
收藏
|
浏览/下载：173/61
|
提交时间：2022/04/02
biodiversity hotspot
climate change
elevational gradient
Himalayan flora
mountain ecosystem
spatiotemporal diversification
SPECIES RICHNESS PATTERNS
QINGHAI-TIBET PLATEAU
SOUTH ASIAN MONSOON
ELEVATIONAL GRADIENT
HENGDUAN MOUNTAINS
CLIMATE-CHANGE
BETA-DIVERSITY
QUATERNARY GLACIATION
GENETIC CONSEQUENCES
ARTIFICIAL DISPERSAL
Estimating climate-induced 'Nowhere to go' range shifts of the Himalayan Incarvillea Juss. using multi-model median ensemble species distribution models
期刊论文
ECOLOGICAL INDICATORS, 2021
作者:
Rana,Santosh Kumar
;
Rana,Hum Kala
;
Luo,Dong
;
Sun,Hang
Adobe PDF(12422Kb)
|
收藏
|
浏览/下载：128/51
|
提交时间：2023/09/08
Natural hybridization among three Rhododendron species (Ericaceae) revealed by morphological and genomic evidence
期刊论文
BMC PLANT BIOLOGY, 2021, 卷号: 21, 期号: 1, 页码: 529
作者:
Zheng,Wei
;
Yan,Li-Jun
;
Burgess,Kevin S.
;
Luo,Ya-Huang
;
Zou,Jia-Yun
;
Qin,Han-Tao
;
Wang,Ji-Hua
;
Gao,Lian-Ming
Adobe PDF(2975Kb)
|
收藏
|
浏览/下载：79/14
|
提交时间：2022/04/02
ddRAD sequencing
Hybrid zone
Morphological trait
Genetic structure
Natural hybridization
Rhododendron
SAGEBRUSH ARTEMISIA-TRIDENTATA
NARROW HYBRID ZONE
ASYMMETRIC HYBRIDIZATION
REPRODUCTIVE ISOLATION
AQUILEGIA-FORMOSA
PINUS-DENSATA
MOUNT-ETNA
SPECIATION
ORIGIN
ADAPTATION
The natural hybridization between species Ligularia nelumbifolia and Cremanthodium stenoglossum (Senecioneae, Asteraceae) suggests underdeveloped reproductive isolation and ambiguous intergeneric boundary
期刊论文
AOB PLANTS, 2021, 卷号: 13, 期号: 2, 页码: plab012
作者:
Hu,Li
;
Yang,Rui
;
Wang,Yue-Hua
;
Gong,Xun
Adobe PDF(1018Kb)
|
收藏
|
浏览/下载：76/16
|
提交时间：2022/04/02
Cremanthodium
ddRAD-seq
F(1)s
Ligularia
natural hybridization
HYBRID SPECIATION
YUNNAN
INTROGRESSION
DISPLACEMENT
RADIATION
VELLEREA
PATTERNS
PROGRAM
STACKS
Natural hybridization between two butterfly bushes in Tibet: dominance of F-1 hybrids promotes strong reproductive isolation
期刊论文
BMC PLANT BIOLOGY, 2021, 卷号: 21, 期号: 1, 页码: 133
作者:
Liao,Rongli
;
Sun,Weibang
;
Ma,Yongpeng
Adobe PDF(3751Kb)
|
收藏
|
浏览/下载：63/4
|
提交时间：2022/04/02
Butterfly bushes
Buddleja
Hybridization
F-1-dominated hybrids
Reproductive isolation
Delimiting species in Basidiomycota: a review
期刊论文
FUNGAL DIVERSITY, 2021, 卷号: 109, 期号: 1, 页码: 181-237
作者:
Cao,Bin
;
Haelewaters,Danny
;
Schoutteten,Nathan
;
Begerow,Dominik
;
Boekhout,Teun
;
Giachini,Admir J.
;
Gorjon,Sergio P.
;
Gunde-Cimerman,Nina
;
Hyde,Kevin D.
;
Kemler,Martin
;
Li,Guo-Jie
;
Liu,Dong-Mei
;
Liu,Xin-Zhan
;
Nuytinck,Jorinde
;
Papp,Viktor
;
Savchenko,Anton
;
Savchenko,Kyryll
;
Tedersoo,Leho
;
Theelen,Bart
;
Thines,Marco
;
Tomsovsky,Michal
;
Toome-Heller,Merje
;
Uron,Judith P.
;
Verbeken,Annemieke
;
Vizzini,Alfredo
;
Yurkov,Andrey M.
;
Zamora,Juan Carlos
;
Zhao,Rui-Lin
浏览
|
Adobe PDF(1700Kb)
|
收藏
|
浏览/下载：78/3
|
提交时间：2022/04/02
Biological species concept
Morphological species concept
Phylogenetic species concept
Taxonomy
INTERNAL TRANSCRIBED SPACER
FRAGMENT-LENGTH-POLYMORPHISMS
FLIGHT MASS-SPECTROMETRY
RUST FUNGI PUCCINIALES
NUCLEAR RIBOSOMAL DNA
MOLECULAR PHYLOGENETIC-RELATIONSHIPS
HEBELOMA-CRUSTULINIFORME COMPLEX
ASSESSING NATURAL RELATIONSHIPS
DESORPTION IONIZATION-TIME
MULTIPLE GENE GENEALOGIES
