KIB OpenIR  > 昆明植物所硕博研究生毕业学位论文
Thesis Advisor郑光植
Degree Grantor中国科学院昆明植物研究所
Place of Conferral中国科学院昆明植物研究所
Degree Discipline植物学
Keyword西洋参 人参 悬浮培养 发酵培养 人参皂甙 寡糖
Other AbstractGinsenosides formation slightly behind the growth curve in suspension culture of quinquefolium and ginseng cells. The growth rate and biomass of suspension culture were the greatest in static liquid culture, suspension culture and fermentation culture. The fermentation culture of quinquefolium cells with a fixed PH value was better than the culture with a changing PH value by itself. The volumetric oxygen transfer coefficient (Kla) values measured in the cultured broths of ginseng and quinquefolium cells were far smaller than that in the fresh medium. These data suggest that the high viscosity of the cultured broths can be one of the problems in scaling up this system. It was found that MS medium minus NH_4NO_3 and with added KNO_3 gave a higher growth ratio and saponin content than regular MS medium or MS minus KNO_3. The growth ratio and saponin content with the medium only added KNO_3 were increased 65.1% and 166.2% separately when compared with the regular MS medium. The saponin content reached 1.58%which was 3 times as high as the control when we applied an osmotic stress by the addition of mannitol to the nutrient medium in fermentation culture of quinquefolium cells. An appropriate agitating cpeed was 60rpm in fermentation culture of quinquefolium cells. Oligosaccharins of Panax ginseng and Dendrum canditum increased both saponin content and growth ratio. Especially, the content of Rg group saponins was well stimulated, which was 1.78 times (added ginseng oligosaccharin) and 2.83 times (added canditum oligosaccharin) as high as the control. Ginsenoside Rb1, Re and Rg1 from quinquefolium cell cultures were isolated and identificated by TLC, ~(13)CNMR, mp, IR, [a]_D~OC, EI-MS and elemental analysis. Saponin compositions of the cultures of quinquefolium cells were almost same with those of the cultivated plants. Studies cn the mass culture of quinquefolium and ginseng cells could well be employed to produce ginsenosides on an industrial scale in the fulture.
Document Type学位论文
Recommended Citation
GB/T 7714
周立刚. 西洋参、人参的细胞大量培养及其皂甙成分的分离和鉴定[D]. 中国科学院昆明植物研究所. 中国科学院昆明植物研究所,1989.
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