滇牡丹的遗传多样性研究
其他题名A Study on the Genetic Diversity of Paeonia delavayi
杨淑达
学位类型硕士
导师龚洵
2005
学位授予单位中国科学院昆明植物研究所
学位授予地点中国科学院昆明植物研究所
学位专业植物学
关键词滇牡丹 遗传多样性 遗传分化 保护
摘要滇牡丹(Paeonia delavavi Franch,)是国家二级保护植物,也是我国西南地区的特有植物。生境的破坏以及人们的大量采挖致使滇牡丹的生存受到了威胁。物种的遗传多样性研究是揭示其进化和适应潜力的基础,并能为制定科学的保护策略和措施、合理开发利用资源提供理论依据。本论文利用分子标记ISSR方法检测了滇牡丹16个自然居群和昆明植物园一个迁地保护居群的遗传变异水平及居群遗传结构,主要结果如下:1.滇牡丹具有较高的遗传变异水平。从100个ISSR引物中筛选出10个用于正式扩增,在取自16个自然居群和一11个迁地保护居群的511个个体中,共扩增到116条带,其中多态带为92条。在居群水平上,多态位点百分率(PPB)为44.61%,Nei’s基因多样性指数(H)和Shannon信息指数(I)分别为0.1657和0.2448。在物种水平上,多态位点百分率(PPB)为79.31%,Nei's基因多样性指数(H)和Shormon信息指数(I)分别为0.2,47和0.4355。这与滇牡月高的形态变异以及生境的多样性相关。2.居群间显著的遗传分化。滇牡丹居群间的遗传分化系数(Gst)达0.4349。这可能是滇牡丹靠弱飞行能力昆虫传粉兼有较强无性繁殖能力、居群间地理趴离较大及其分布区复杂多样的地形和生境等因素综合作用的结果。3.迁地保护居群(KM)的遗传多样性处于中间水平(PPB=57.76%,H=0.2070,I=0.3063)。为保存更多的遗传变异,取样时应该把所有居群考虑在内,特别对遗传多样性较高的GZ和NPH居群应给予足够的重视。结合野外调查和前人的研究,表明滇牡丹拥有较高的遗传、形态和生境的多样性,具有较强的适应能力和进化潜力,并不处于濒危境地:最主要的威胁司”能是人为造成的生境破坏和滥挖滥采。
其他摘要Paeonia delavayi. an endemic species to Southwest China, has been given the Second Grade Conservation Status in China. In recent years. Paeonia delavayi has been increasingly threatened by the destruction of its habitats and exploitation for both medicine and ornament. Genetic diversity in a species is the potential for its evolution and adaptation, and the understanding of genetic variation is the basis for utilization of plant genetic resources and essential for the establishment of effective and efficient conservation strategies for endangered plant. This study revealed the level of genetic variation and the genetic structure of Paeonia delavayi, which were all detected by inter-simple sequence repeat marker (ISSR). The main results are highlighted as follows: 1. Paeonia delavayi maintained a relatively high level of genetic variation. 10 informative and reliable ISSR primers, prescreened from 100 primers, produced 116 bands across a total of 511 individuals from 16 natural populations and one ex situ conserved population in Kunming Botanical Garden. Of them. 92 ISSR loci were polymorphic. A relatively high level of intraspecific genetic diversity was revealed: PPB = 44.61%, H = 0.1657 and / = 0.2448 at population level; PPB = 79.31%. H = 0.2947 and / = 0.4355 at species level. The high level of genetic diversity is highly correlated with its morphological variation and diverse habitats.2. The Gst = 0.4349 indicated a high degree of genetic differentiation occurred among P. delavayi populations. This genetic structure is due to the combined effects of breeding system, large geographical distance between populations, special distributions and limited gene flow among populations. 3. The ex situ conserved population in Kunming Botanical Garden (KM) preserved a medium level of genetic diversity (PPB = 57.76%. H= 0.2070, / = 0.3063). In order to conserve more genetic diversity, all populations, especially Geza population (GZ) and Napahai population (NPH) which hold more genetic variations, should be taken into account in the collection of ex situ population. The results of genetic diversity study, associated with field survey and previous investigations, suggest that P. delavayi is not in endangered status for holding high level of genetic and morphological variations and diverse habitats, and possessing strong potential for its evolution and adaptation. The main factor threatening its survival is the fragment of its habitats and over-collection.
页数50
语种中文
文献类型学位论文
条目标识符http://ir.kib.ac.cn/handle/151853/796
专题昆明植物所硕博研究生毕业学位论文
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GB/T 7714
杨淑达. 滇牡丹的遗传多样性研究[D]. 中国科学院昆明植物研究所. 中国科学院昆明植物研究所,2005.
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