Establishing the first reference library for utilizing high-throughput sequencing technologies in identifying medicinal and endangered Paris species (Melanthiaceae)

doi:10.1016/j.indcrop.2024.118871

KIB OpenIR

	Establishing the first reference library for utilizing high-throughput sequencing technologies in identifying medicinal and endangered Paris species (Melanthiaceae)
	Miao, Ke; Wang, Tinglu; Tang, Lilei; Hou, Luxiao; Ji, Yunheng
	2024
发表期刊	INDUSTRIAL CROPS AND PRODUCTS
ISSN	0926-6690
卷号	218 页码:118871
摘要	The genus Paris (Melanthiaceae) holds significant pharmaceutical importance and is subject to high conservation concerns. Accurate species identification plays a crucial role in effectively addressing issues related to the exploitation, utilization, and conservation of this plant lineage. Recently, high-throughput sequencing technologies have been employed to generate complete plastid genomes (plastomes) and nuclear ribosomal DNA (nrDNA) arrays as molecular markers for plant species identification. However, the lack of reference libraries poses a substantial challenge to the widespread application of this approach. In this study, we established a comprehensive reference library consisting of complete plastomes and nrDNA arrays from 153 individuals representing all medicinal and endangered Paris species. The robustness of the reference sequences in discriminating Paris species was assessed through phylogenetic and genetic distance analyses. The results demonstrate that both complete plastome and nrDNA reference sequences exhibit a high degree of consistency with species boundaries as defined by recent taxonomic revisions to the genus, thereby facilitating unambiguous species identification. The favorable performance of this reference library underscores its invaluable significance as a genetic resource for the efficient monitoring and regulation of the collection and trade of medicinal and endangered Paris species, along with associated plant products, through the utilization of high-throughput sequencing technologies. The findings of this study will make a significant contribution to enhancing the efficacy and safety assurance of Rhizoma Paridis products, as well as supporting conservation efforts for endangered Paris species.
DOI	10.1016/j.indcrop.2024.118871
WOS记录号	WOS:001256289400001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/76360
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Miao, Ke,Wang, Tinglu,Tang, Lilei,et al. Establishing the first reference library for utilizing high-throughput sequencing technologies in identifying medicinal and endangered Paris species (Melanthiaceae)[J]. INDUSTRIAL CROPS AND PRODUCTS,2024,218:118871.
APA	Miao, Ke,Wang, Tinglu,Tang, Lilei,Hou, Luxiao,&Ji, Yunheng.(2024).Establishing the first reference library for utilizing high-throughput sequencing technologies in identifying medicinal and endangered Paris species (Melanthiaceae).INDUSTRIAL CROPS AND PRODUCTS,218,118871.
MLA	Miao, Ke,et al."Establishing the first reference library for utilizing high-throughput sequencing technologies in identifying medicinal and endangered Paris species (Melanthiaceae)".INDUSTRIAL CROPS AND PRODUCTS 218(2024):118871.

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