Single-cell RNA sequencing reveals dynamics of gene expression for 2D elongation and 3D growth in Physcomitrium patens

doi:10.1016/j.celrep.2024.114524

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	Single-cell RNA sequencing reveals dynamics of gene expression for 2D elongation and 3D growth in Physcomitrium patens
	Chen, Zexi; Wang, Wenbo; Zhou, Shizhao; Ding, Lulu; Xu, Zhanwu; Sun, Xuwu; Huo, Heqiang; Liu, Li
	2024
发表期刊	CELL REPORTS
ISSN	2211-1247
卷号	43 期号:8 页码:114524
摘要	The transition from two-dimensional (2D) to 3D growth likely facilitated plants to colonize land, but its heterogeneity is not well understood. In this study, we utilized single-cell RNA sequencing to analyze the moss Physcomitrium patens, whose morphogenesis involves a transition from 2D to 3D growth. We profiled over 17,000 single cells covering all major vegetative tissues, including 2D filaments (chloronema and caulonema) and 3D structures (bud and gametophore). Pseudo time analyses revealed larger numbers of candidate genes that determine cell fates for 2D tip elongation or 3D bud differentiation. Using weighted gene co-expression network analysis, we identified a module that connects b-type carbonic anhydrases (bCAs) with auxin. We further validated the cellular expression patterns of bCAs and demonstrated their roles in 3D gametophore development. Overall, our study provides insights into cellular heterogeneity in a moss and identifies molecular signatures that underpin the 2D-to-3D growth transition at single-cell resolution.
DOI	10.1016/j.celrep.2024.114524
WOS记录号	WOS:001279379700001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/76337
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Chen, Zexi,Wang, Wenbo,Zhou, Shizhao,et al. Single-cell RNA sequencing reveals dynamics of gene expression for 2D elongation and 3D growth in Physcomitrium patens[J]. CELL REPORTS,2024,43(8):114524.
APA	Chen, Zexi.,Wang, Wenbo.,Zhou, Shizhao.,Ding, Lulu.,Xu, Zhanwu.,...&Liu, Li.(2024).Single-cell RNA sequencing reveals dynamics of gene expression for 2D elongation and 3D growth in Physcomitrium patens.CELL REPORTS,43(8),114524.
MLA	Chen, Zexi,et al."Single-cell RNA sequencing reveals dynamics of gene expression for 2D elongation and 3D growth in Physcomitrium patens".CELL REPORTS 43.8(2024):114524.

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