Sensitive, precise fingerprint profiling for monosaccharide analysis of Bacillus Calmette-Guerin polysaccharide and nucleic acid isolates

doi:10.1016/j.carres.2024.109124

KIB OpenIR

	Sensitive, precise fingerprint profiling for monosaccharide analysis of Bacillus Calmette-Guerin polysaccharide and nucleic acid isolates
	Luo, Lan; Lin, Lisha; Huang, Sheng; Zhou, Yunxi; Yang, Shengmei; Zhu, Yan; Zhang, Lanyan; Xiong, Donghua; Wu, Yongsheng; Wu, Mingyi
	2024
发表期刊	CARBOHYDRATE RESEARCH
ISSN	0008-6215
卷号	540 页码:109124
摘要	A sensitive and precise HPLC-DAD method with pre-column PMP derivatization was established and validated, for analyzing the polysaccharides in Bacillus Calmette-Gu & eacute;rin polysaccharide and nucleic acid (BCG-PSN) isolates, after acid hydrolysis. And the HPLC fingerprint profiling was used to analyze its monosaccharide composition. The monosaccharide concentration-peak area calibration curve was of good linearity (R-2 > 0.99), over the range of 0.016-0.08 mg/mL for mannose or 0.24-1.20 mg/mL for glucose, with high recovery of 93-105 % for quality control samples. The intra-day RSD values of mannose and glucose concentration were less than 2.5 % and 2.1 %, respectively, and their inter-day RSD values were less than 4.3 % and 2.2 %, respectively, and remained stable for up to 14 days. This method also remained durable against changes in chromatographic parameters, but it's susceptible to the flow rate of mobile phase. Additionally, the method was applied to analyze the content of mannose and glucose in 22 batches BCG-PSN powder and 17 batches BCG-PSN injection. The results showed that the HPLC-DAD fingerprint spectra of all the BCG-PSN powder and BCG-PSN injection samples had a high degree of similarity, with the similar indexes up to 0.999 and 0.998, respectively. The HPLC-DAD method with pre-column PMP derivatization is highly rapid, effective, visual, and accurate for determination of monosaccharide contents. The validated method was successfully applied to the analysis of polysaccharide in both BCG-PSN powder and injection.
DOI	10.1016/j.carres.2024.109124
WOS记录号	WOS:001301358300001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/76336
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Luo, Lan,Lin, Lisha,Huang, Sheng,et al. Sensitive, precise fingerprint profiling for monosaccharide analysis of Bacillus Calmette-Guerin polysaccharide and nucleic acid isolates[J]. CARBOHYDRATE RESEARCH,2024,540:109124.
APA	Luo, Lan.,Lin, Lisha.,Huang, Sheng.,Zhou, Yunxi.,Yang, Shengmei.,...&Wu, Mingyi.(2024).Sensitive, precise fingerprint profiling for monosaccharide analysis of Bacillus Calmette-Guerin polysaccharide and nucleic acid isolates.CARBOHYDRATE RESEARCH,540,109124.
MLA	Luo, Lan,et al."Sensitive, precise fingerprint profiling for monosaccharide analysis of Bacillus Calmette-Guerin polysaccharide and nucleic acid isolates".CARBOHYDRATE RESEARCH 540(2024):109124.

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