Human transferrin receptor can mediate SARS-CoV-2 infection

doi:10.1073/pnas.2317026121

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	Human transferrin receptor can mediate SARS-CoV-2 infection
	Liao, Zhiyi; Wang, Chaoming; Tang, Xiaopeng; Yang, Mengli; Duan, Zilei; Liu, Lei; Lu, Shuaiyao; Ma, Lei; Cheng, Ruomei; Wang, Gan; Liu, Hongqi; Yang, Shuo; Xu, Jingwen; Tadese, Dawit Adisu; Mwangi, James; Kamau, Peter Muiruri; Zhang, Zhiye; Yang, Lian; Liao, Guoyang; Zhao, Xudong; Peng, Xiaozhong; Lai, Ren
	2024
发表期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN	0027-8424
卷号	121 期号:10 页码:e2317026121
摘要	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has been detected in almost all organs of coronavirus disease-19 patients, although some organs do not express angiotensin-converting enzyme-2 (ACE2), a known receptor of SARS-CoV-2, implying the presence of alternative receptors and/or co-receptors. Here, we show that the ubiquitously distributed human transferrin receptor (TfR), which binds to diferric transferrin to traffic between membrane and endosome for the iron delivery cycle, can ACE2-independently mediate SARS-CoV-2 infection. Human, not mouse TfR, interacts with Spike protein with a high affinity (K-D similar to 2.95 nM) to mediate SARS-CoV-2 endocytosis. TfR knock-down (TfR-deficiency is lethal) and overexpression inhibit and promote SARS-CoV-2 infection, respectively. Humanized TfR expression enables SARS-CoV-2 infection in baby hamster kidney cells and C57 mice, which are known to be insusceptible to the virus infection. Soluble TfR, Tf, designed peptides blocking TfR-Spike interaction and anti-TfR antibody show significant anti-COVID-19 effects in cell and monkey models. Collectively, this report indicates that TfR is a receptor/co-receptor of SARS-CoV-2 mediating SARS-CoV-2 entry and infectivity by likely using the TfR trafficking pathway.
DOI	10.1073/pnas.2317026121
WOS记录号	WOS:001208094600007
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/76285
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Liao, Zhiyi,Wang, Chaoming,Tang, Xiaopeng,et al. Human transferrin receptor can mediate SARS-CoV-2 infection[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2024,121(10):e2317026121.
APA	Liao, Zhiyi.,Wang, Chaoming.,Tang, Xiaopeng.,Yang, Mengli.,Duan, Zilei.,...&Lai, Ren.(2024).Human transferrin receptor can mediate SARS-CoV-2 infection.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,121(10),e2317026121.
MLA	Liao, Zhiyi,et al."Human transferrin receptor can mediate SARS-CoV-2 infection".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 121.10(2024):e2317026121.

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