Enrichment process, structural prediction, isolation, in vitro cytotoxic and anti-inflammatory effects of triterpenoid saponins in Camellia japonica L. leaves water extract through UPLC-Q-TOF based mass spectrometry similarity networking

doi:10.1016/j.foodchem.2024.138360

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	Enrichment process, structural prediction, isolation, in vitro cytotoxic and anti-inflammatory effects of triterpenoid saponins in Camellia japonica L. leaves water extract through UPLC-Q-TOF based mass spectrometry similarity networking
	Liu, Hui; Bi, Lisha; Chen, Qirun; He, Xiaozhi; Yan, Huan; Ni, Wei; Wu, Wenjuan; He, Li; Liu, Haiyang
	2024
发表期刊	FOOD CHEMISTRY
ISSN	0308-8146
卷号	441 页码:138360
摘要	Camellia japonica L. is rich in bioactive compounds, but its health-enhancing potential is often overshadowed by its ornamental value. Notably, triterpenoid saponins are prominent due to their surfactant properties. MolNetEnhancer revealed 537 compounds in C. japonica leaves water extract, classified into 32 categories, including 38 triterpenoid saponins. To enrich triterpenoid saponins, the process of D101 resin chromatography was employed. Molecular networking analysis based on UPLC-Q-TOF and quantitative analysis based on HPLC revealed saponins concentrated in fractions 3 and 4 (68.3% transfer). MS2LDA and NAP predicted structures for 38 triterpenoid saponins, revealing nearly half of them are potential new compounds. Comprehensive chromatographic and spectroscopic methods were used for purification and structural illustration of triterpenoid saponins, yielding 13, including 7 new compounds. Statistical analysis and in vitro assays revealed the cytotoxic and anti-inflammatory activities of these triterpenoid saponins played a crucial role in the anticancer effects.
DOI	10.1016/j.foodchem.2024.138360
WOS记录号	WOS:001164602500001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/76090
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Liu, Hui,Bi, Lisha,Chen, Qirun,et al. Enrichment process, structural prediction, isolation, in vitro cytotoxic and anti-inflammatory effects of triterpenoid saponins in Camellia japonica L. leaves water extract through UPLC-Q-TOF based mass spectrometry similarity networking[J]. FOOD CHEMISTRY,2024,441:138360.
APA	Liu, Hui.,Bi, Lisha.,Chen, Qirun.,He, Xiaozhi.,Yan, Huan.,...&Liu, Haiyang.(2024).Enrichment process, structural prediction, isolation, in vitro cytotoxic and anti-inflammatory effects of triterpenoid saponins in Camellia japonica L. leaves water extract through UPLC-Q-TOF based mass spectrometry similarity networking.FOOD CHEMISTRY,441,138360.
MLA	Liu, Hui,et al."Enrichment process, structural prediction, isolation, in vitro cytotoxic and anti-inflammatory effects of triterpenoid saponins in Camellia japonica L. leaves water extract through UPLC-Q-TOF based mass spectrometry similarity networking".FOOD CHEMISTRY 441(2024):138360.

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