|其他摘要||Chemical constituents and resources of five medical plants from Yunnan province were studied.
The dissertation consists of seven parts.
The first part concerned the studies on Amomum tsao-ko (caoguo in Chinese), which is mainly distributed in Yunnan Province. It is not only a TCM but also a famous spice used for cooking. 46 compounds were isolated from the methanol extract of dry fruit of Amomum tsao-ko by means of materials such as normal phase, reverse phase, Sephadex LH-20, and macroporous resin combined with normal pressure and middle pressure column chromatograph. Their structures were identified based on various spectroscopy including MS, 1D NMR, 2D NMR, IR, UV and compared with literature values. 34 compounds were isolated from the plant for the first time, of which 4 compounds were new constituents. Their structures include diarylheptanoids, terpenes, flavonoids, and simple phenols.
Their antixidative activity was tested with several constituents possessing higher effect than that of VC. The structure-activity relationships were also discussed based on the results.
The preparation of the fruit of Amomum tsao-ko is a severe problem. It was invested by related section that improper preparation can led to the occurrence of benzo[a]pyrene that may cause cancer. Therefore the volatile oil from the steam distillation of Amomum tsao-ko from Maguan County prepared by three kinds of different methods were subjected to the GC-MS analysis with most peaks identified by comparison with the database. The results showed that they were very similar to each other with 1, 8 cineol as main constituent accounting for more than half of the weight of volatile oil. benzo[a]pyrene was not detected in the test. At last, the fingerprint of volatile oil from Amomum tsao-ko was discussed based on the results.
A math-model method was used to compare the weigh, volume, density of fruits of Amomum tsao-ko from different regions and with different shapes.
A HPLC fingerprint analysis method of non-volatile constituents from Amomum tsao-ko was established with 24 constituents were being confirmed by comparison with their retention time and UV absorbtion.
The second part dealed with two species of substitute for Sanghuang, a famous anti-cancer fungus belonging to Genus Phellinus, which is often confused with other species. 7 known compounds were obtained from methanol extract of Phellinus torulosus with their structures including lanosterol-type triterpenes and simple phenols characterized based on their spectroscopic properties. As for Phellinus linteus, 3 known compounds were first isolated from its methanol extract and their structures were established by spectroscopic means.
Chemical investigations of Baphicacanthus cusia were researched in the third part. Its root was a resource of Traditional Chinese Medicine “Ban Lan Gen” used for virus diseases. Baphicacanthus cusia was the only species of “Nan Ban Lan Gen” in the Chinese Pharmacopoeia of 2005 version. 10 compounds were isolated from the methanol extract of its roots with their structure including fatty acids, lignans, steroids and polyphenols.
In the chemical investigation of Balanophora harlandii Hook. F, a folk medicine, 19 compounds were isolated from its plant with a new compound. Their structures include hydrolysable tannins, flavonoids, polyphenols and phenylpropanoids. Their antixidative activity was tested.
In the fifth part, 22 species of Southern Medicine resources in Yunnan Province and Zingiberaceae plants in Tibetan Medicine were listed.
20 species of Zingiberaceae plants in Tibetan Medicine were also listed in the six parts.
The seven parts was a review. Research development such as structures and pharmacology of diarylheptanoids from 1990-2006 were reviewed. Diarylheptanoids is a general name for the compounds which have two benzene rings and a heptane-chain as skeleton. Structures of 205 diarylheptanoids and their literatures were listed. Their Structures can be divided into five types: linear-diarylheptanoids, cyclodiarylheptanoids, dimer of diarylheptanoids, diarylheptanoids bonding with flavonoids and special diarylheptanoids according to their molecular structures. Their pharmacological actions included antioxidation, anticancer, antiinflammation, antiemetic and so on.|