Transcriptome data reveals the conservation genetics of Cypripedium forrestii, a plant species with extremely small populations endemic to Yunnan, China

doi:10.3389/fpls.2024.1303625

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	Transcriptome data reveals the conservation genetics of Cypripedium forrestii, a plant species with extremely small populations endemic to Yunnan, China
	Lin, Liewen; Cai, Lei; Huang, Hua; Ming, Shengping; Sun, Weibang
	2024
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	15 页码:1303625
摘要	The Cypripedium forrestii is an orchid species with extremely small populations (PSESP) in Yunnan, China. C. forrestii is range-restricted and less-studied than many orchid species, and it is exposed to various threats to its survival. We investigated its potential habitats and collected 52 samples from eight locations, as well as two outgroup species for reference. We developed genetic markers (SNPs) for C. forrestii based on transcriptome sequencing (RNA-seq) data, and analyzed the genetic diversity, population structure, gene flow and demographic history of C. forrestii in detail. C. forrestii is a taxonomically independent species to protect. We found that the genetic diversity of C. forrestii was very low (1.7e-4) compared with other endangered species. We identified three genetic clusters, and several populations with distinct genetic backgrounds. Most genetic diversity was found within sampling sites (87.87%) and genetic clusters (91.39%). Gene flow has been greatly limited over the most recent generations, probably due to geographical distance, historical climate change and habitat fragmentation. We also detected a severe bottleneck event brought about by the recent population constraints. These factors, together with its reproductive characteristics, contribute to the population fragmentation and low genetic diversity of C. forrestii. Based on our findings, we suggest an integrative conservation strategy to protect and recover the genetic diversity of C. forrestii and a further comprehensive study of its ecological traits in the future.
DOI	10.3389/fpls.2024.1303625
WOS记录号	WOS:001161386800001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75830
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Lin, Liewen,Cai, Lei,Huang, Hua,et al. Transcriptome data reveals the conservation genetics of Cypripedium forrestii, a plant species with extremely small populations endemic to Yunnan, China[J]. FRONTIERS IN PLANT SCIENCE,2024,15:1303625.
APA	Lin, Liewen,Cai, Lei,Huang, Hua,Ming, Shengping,&Sun, Weibang.(2024).Transcriptome data reveals the conservation genetics of Cypripedium forrestii, a plant species with extremely small populations endemic to Yunnan, China.FRONTIERS IN PLANT SCIENCE,15,1303625.
MLA	Lin, Liewen,et al."Transcriptome data reveals the conservation genetics of Cypripedium forrestii, a plant species with extremely small populations endemic to Yunnan, China".FRONTIERS IN PLANT SCIENCE 15(2024):1303625.

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