Isolation and Biosynthesis of Phenazine-Polyketide Hybrids from Streptomyces sp. KIB-H483

doi:10.1021/acs.jnatprod.2c00067

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	Isolation and Biosynthesis of Phenazine-Polyketide Hybrids from Streptomyces sp. KIB-H483
	Wang, Zhiyan; Yang, Feng-Xian; Liu, Chongxi; Wang, Li; Qi, Yuxin; Cao, Minghang; Guo, Xiaowei; Li, Jie; Huang, Xueshuang; Yang, Jing; Huang, Sheng-Xiong
	2022
发表期刊	JOURNAL OF NATURAL PRODUCTS
卷号	85 期号:5 页码:1324-1331
摘要	A phenazine-polyketide hybrid compound, nexphenazine A (1), was isolated from Streptomyces sp. KIB-H483. The bioinformatic analysis of the draft genome of the producing strain and gene inactivation experiments revealed that the biosynthesis of 1 involves a phenazine-polyketide hybrid gene cluster. The abolished production of 1 as well as the accumulation of shunt metabolites 4-7 in mutant strain Delta npzI revealed the key role of the npzI gene, which encodes an NAD(P)H-dependent ketoreductase, in nexphenazine biosynthesis. The structures and absolute configurations of the isolated intermediates were established on the basis of spectroscopic data analysis, singlecrystal X-ray diffraction, chiral chromatography, and chemical conversion experiments. NpzI exhibited stereochemical selectivity in reducing the carbonyl group of 4. Nexphenazine biosynthesis is proposed to involve a condensation of the carboxyl group of phenazine with one molecule of methylmalonyl-CoA by a type I PKS, followed by a ketone reduction by NpzI and an unknown methylation reaction.
关键词	GENE-CLUSTER ANTIBIOTICS CLONING
DOI	10.1021/acs.jnatprod.2c00067
收录类别	SCI
WOS记录号	WOS:000806554600015
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75665
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Wang, Zhiyan,Yang, Feng-Xian,Liu, Chongxi,et al. Isolation and Biosynthesis of Phenazine-Polyketide Hybrids from Streptomyces sp. KIB-H483[J]. JOURNAL OF NATURAL PRODUCTS,2022,85(5):1324-1331.
APA	Wang, Zhiyan.,Yang, Feng-Xian.,Liu, Chongxi.,Wang, Li.,Qi, Yuxin.,...&Huang, Sheng-Xiong.(2022).Isolation and Biosynthesis of Phenazine-Polyketide Hybrids from Streptomyces sp. KIB-H483.JOURNAL OF NATURAL PRODUCTS,85(5),1324-1331.
MLA	Wang, Zhiyan,et al."Isolation and Biosynthesis of Phenazine-Polyketide Hybrids from Streptomyces sp. KIB-H483".JOURNAL OF NATURAL PRODUCTS 85.5(2022):1324-1331.

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