Evaluation of the in vitro antioxidant and antitumor activity of extracts from Camellia fascicularis leaves

doi:10.3389/fchem.2022.1035949

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	Evaluation of the in vitro antioxidant and antitumor activity of extracts from Camellia fascicularis leaves
	Peng, Xiaowei; He, Xuhua; Tang, Junrong; Xiang, Jianying; Deng, Jia; Kan, Huan; Zhang, Yingjun; Zhang, Guiliang; Zhao, Ping; Liu, Yun
	2022
发表期刊	FRONTIERS IN CHEMISTRY
卷号	10 页码:1034785
摘要	Camellia fascicularis is a unique plant rich in bioactive components. However, the isolation of the active substances in C. fascicularis leaves via sequential extraction with solvents of different polarity and the determination of their antioxidant and antitumor activities have not been reported. In this study, the total methanol extract of C. fascicularis leaves was sequentially extracted with different polar solvents, and the corresponding petroleum ether extract (PEE), ethyl acetate extract (EAE), and water extract (WE) were analyzed for their contents in active substances such as flavonoids, polyphenols, polysaccharides, and saponins. The antioxidant ability of the polar extracts was investigated by determining their reducing power and the radical scavenging rate on 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and hydroxyl radicals, and CCK-8 and Annexin-FITC/propidium iodide staining assays were conducted to investigate their inhibitory effects on HCCLM6 and HGC27 tumor cells. The results showed that PEE had a high saponin content of 197.35 +/- 16.21 mg OAE/g, while EAE and WE exhibited a relatively higher polysaccharide content of 254.37 +/- 1.99 and 373.27 +/- 8.67 mg GE/g, respectively. The EAE demonstrated the greatest reducing power and the strongest clearing abilities on ABTS and DPPH radicals with respective EC50 values of 343.45 +/- 20.12 and 14.07 +/- 0.06 mu g/ml. Moreover, the antitumor ability of the different polar extracts was dose-dependent, with WE showing the most potent inhibitory ability against HCCLM6 and HGC27 cells.
关键词	Camellia fascicularis extracts active substances antioxidant antitumor CELASTRUS-ORBICULATUS EXTRACT ANTIOXIDANT ACTIVITIES STRUCTURAL CHARACTERISTICS ASSISTED EXTRACTION VITRO ANTIOXIDANT AQUEOUS EXTRACT POLYSACCHARIDES POLYPHENOLS METASTASIS APOPTOSIS
DOI	10.3389/fchem.2022.1035949
收录类别	SCI
WOS记录号	WOS:000884911400001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75658
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Peng, Xiaowei,He, Xuhua,Tang, Junrong,et al. Evaluation of the in vitro antioxidant and antitumor activity of extracts from Camellia fascicularis leaves[J]. FRONTIERS IN CHEMISTRY,2022,10:1034785.
APA	Peng, Xiaowei.,He, Xuhua.,Tang, Junrong.,Xiang, Jianying.,Deng, Jia.,...&Liu, Yun.(2022).Evaluation of the in vitro antioxidant and antitumor activity of extracts from Camellia fascicularis leaves.FRONTIERS IN CHEMISTRY,10,1034785.
MLA	Peng, Xiaowei,et al."Evaluation of the in vitro antioxidant and antitumor activity of extracts from Camellia fascicularis leaves".FRONTIERS IN CHEMISTRY 10(2022):1034785.

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