Activity Assay of Amylase and Xylanase Is Available for Quantitative Assessment of Strain Aging in Cultivated Culinary-Medicinal Morchella Mushrooms (Ascomycotina)

doi:10.1615/IntJMedMushrooms.2022046420

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	Activity Assay of Amylase and Xylanase Is Available for Quantitative Assessment of Strain Aging in Cultivated Culinary-Medicinal Morchella Mushrooms (Ascomycotina)
	He,Peixin; Chen,Zhuo; Men,Ying; Wang,Miaomiao; Wang,Wensheng; Liu,Wei
	2023
发表期刊	INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS
ISSN	1940-4344
卷号	25 期号:1 页码:57-64
摘要	Strain aging has been mainly contributing to the uncertainty of Morchella farming. The situation calls for urgent quantitative assessment of strain aging in cultivated Morchella mushrooms. In this paper, systemic senescence of the productive strains of M. eximia, M. importuna, and M. sextelata was achieved through successive subculturing to provide subcultures with different degree of aging for further studies. Then the quantitative assessment of morel strain aging was conducted by activity assay of amylase and xylanase using dinitrosalicylic acid (DNS) method. The results suggested that both activity of amylase and xylanase decreased along with the rise of subculture times. Meanwhile, the correlation between xylanase activity and time of subculturing in the tested morel strains was higher than that of amylase assay. Consequently, assay of amylase and xylanase activity by DNS method can be used in the quantitative assessment of morel strain aging, and assay of xylanase activity is the better alternative. The work will improve the settlement of uncertainty in the morel industry and thus be beneficial for stable development of morel farming.
关键词	Ascomycota Morchella senescence successive culturing medicinal mushrooms
学科领域	Biochemistry & Molecular Biology ; Plant Sciences ; Mycology ; Pharmacology & Pharmacy
DOI	10.1615/IntJMedMushrooms.2022046420
收录类别	SCI
WOS记录号	WOS:000905673100005
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75614
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	He,Peixin,Chen,Zhuo,Men,Ying,et al. Activity Assay of Amylase and Xylanase Is Available for Quantitative Assessment of Strain Aging in Cultivated Culinary-Medicinal Morchella Mushrooms (Ascomycotina)[J]. INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS,2023,25(1):57-64.
APA	He,Peixin,Chen,Zhuo,Men,Ying,Wang,Miaomiao,Wang,Wensheng,&Liu,Wei.(2023).Activity Assay of Amylase and Xylanase Is Available for Quantitative Assessment of Strain Aging in Cultivated Culinary-Medicinal Morchella Mushrooms (Ascomycotina).INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS,25(1),57-64.
MLA	He,Peixin,et al."Activity Assay of Amylase and Xylanase Is Available for Quantitative Assessment of Strain Aging in Cultivated Culinary-Medicinal Morchella Mushrooms (Ascomycotina)".INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS 25.1(2023):57-64.

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