Structure characterization of a highly branched galactan from the slug Vaginulus alte and its utilization by human gut microbiota

doi:10.1016/j.ijbiomac.2023.123935

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	Structure characterization of a highly branched galactan from the slug Vaginulus alte and its utilization by human gut microbiota
	Zuo,Zhichuang; He,Zhicheng; Ma,Yan; Xu,Chen; Tao,Xuelin; Ning,Zimo; Gao,Li; Rehemiding,Ailainuer; Gao,Na; Zhao,Jinhua
	2023
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	1879-0003
卷号	236 页码:123935
摘要	The slug Vaginulus alte is used as folk medicine in China, but the structure and activities of its galactan com-ponents remain to be clarified. Here, the galactan from V. alte (VAG) was purified. The Mw of VAG was determined as-28.8 kDa. Chemical composition analysis showed that VAG was composed of D-galactose (75 %) and L-galactose (25 %). To elucidate its precise structure, a series of disaccharides and trisaccharides were pu-rified from mild acid hydrolyzed VAG and their structures were characterized by 1D/2D NMR spectroscopy. Based on methylation analysis and structural analysis of oligosaccharides, VAG was elucidated as a highly branched polysaccharide and mainly composed of (1 -> 6)-or (1 -> 3)-linked beta-D-galactose, and distinct (1 -> 2)-linked alpha-L-galactose. The investigation of probiotic effects in vitro revealed that VAG could promote the growth of B. thetaiotaomicron and B. ovatus, while had no effect on the growth of L. acidophilus, L. rhamnosus, B. longum subsp. infantis and B. animalis subsp. lactis, but dVAG-3 with Mw-1.0 kDa could promote the growth of L. acidophilus. These results will provide insights into specific structures and functions of polysaccharides from the V. alte.
关键词	Slug Galactan Chemical structure Oligosaccharide Human gut microbiota SULFATED GALACTANS HELIX-POMATIA POLYSACCHARIDES BIOACTIVITY HYDROLYSIS
学科领域	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
DOI	10.1016/j.ijbiomac.2023.123935
收录类别	SCI
WOS记录号	WOS:000959437500001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75579
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Zuo,Zhichuang,He,Zhicheng,Ma,Yan,et al. Structure characterization of a highly branched galactan from the slug Vaginulus alte and its utilization by human gut microbiota[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2023,236:123935.
APA	Zuo,Zhichuang.,He,Zhicheng.,Ma,Yan.,Xu,Chen.,Tao,Xuelin.,...&Zhao,Jinhua.(2023).Structure characterization of a highly branched galactan from the slug Vaginulus alte and its utilization by human gut microbiota.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,236,123935.
MLA	Zuo,Zhichuang,et al."Structure characterization of a highly branched galactan from the slug Vaginulus alte and its utilization by human gut microbiota".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 236(2023):123935.

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