Integrating morphology, molecular phylogeny and chemotaxonomy for the most effective authentication in Chinese Rubia with insights into origin and distribution of characteristic Rubiaceae-type cyclopeptides

doi:10.1016/j.indcrop.2022.115775

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	Integrating morphology, molecular phylogeny and chemotaxonomy for the most effective authentication in Chinese Rubia with insights into origin and distribution of characteristic Rubiaceae-type cyclopeptides
	Zhang,Xuejia; Yang,Li-E.; Hu,Yanyun; Wu,Xingdong; Wang,Zhe; Miao,Yuanyuan; Sun,Hang; Nie,Zelong; Tan,Ninghua
	2023
发表期刊	INDUSTRIAL CROPS AND PRODUCTS
ISSN	1872-633X
卷号	191 期号:B 页码:115775
摘要	The Rubia is one of important medicinal genera of the family Rubiaceae, widely distributed in the Old World with a great chemical diversity of quinones, cyclopeptides and triterpenoids. It has recently aroused us the most attention for its utilization as multi-origin resources and potential anti-tumor constituents of Rubiaceae-type cyclopeptides (RAs). But a thorough identification and discrimination of the Rubia species is challenging due to multi-species with very similar appearance. In this study, a synthesis of morphology, molecular phylogeny and chemotaxonomy was used to identification and discrimination of the Rubia species. DNA barcoding based on eight chloroplast markers and one nuclear ITS region was firstly applied to reconstruct its phylogenetic history. Sect. Rubia and sect. Oligoneura were clearly supported, but could not completely resolve species relationships within sect. Oligoneura. UHPLC-QTOF-MS technique was further optimized for non-targeted metabolomic analysis with multivariate statistical analysis in both supervised and unsupervised manners to distinguish patterns among the eight main populations. Variable importance in projection (VIP) revealed that triterpenes and RAs were the main specific chemotaxonomic markers. The origin and distribution center of the characteristic RAs in the Rubia species was inferred to be in Southwest (SW) China, which is also the hotspot for Rubia plants biodiversity. Our results highlighted a synthesis of morphology, molecular phylogeny and chemotaxonomy is the most effective strategy for authentication of complex plants (such as Rubia), and also provided insights into the exploration and optimization of high-quality germplasm resource for RAs.
关键词	Rubia Molecular phylogeny Chemotaxonomy Authentication Rubiaceae-type cyclopeptides ANTITUMOR CYCLIC HEXAPEPTIDES ENDOPHYTIC FUNGUS SECONDARY METABOLITES METABOLOMICS EVOLUTION DIVERSIFICATION GENUS
学科领域	Agriculture
DOI	10.1016/j.indcrop.2022.115775
收录类别	SCI
WOS记录号	WOS:000993813000001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75572
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Zhang,Xuejia,Yang,Li-E.,Hu,Yanyun,et al. Integrating morphology, molecular phylogeny and chemotaxonomy for the most effective authentication in Chinese Rubia with insights into origin and distribution of characteristic Rubiaceae-type cyclopeptides[J]. INDUSTRIAL CROPS AND PRODUCTS,2023,191(B):115775.
APA	Zhang,Xuejia.,Yang,Li-E..,Hu,Yanyun.,Wu,Xingdong.,Wang,Zhe.,...&Tan,Ninghua.(2023).Integrating morphology, molecular phylogeny and chemotaxonomy for the most effective authentication in Chinese Rubia with insights into origin and distribution of characteristic Rubiaceae-type cyclopeptides.INDUSTRIAL CROPS AND PRODUCTS,191(B),115775.
MLA	Zhang,Xuejia,et al."Integrating morphology, molecular phylogeny and chemotaxonomy for the most effective authentication in Chinese Rubia with insights into origin and distribution of characteristic Rubiaceae-type cyclopeptides".INDUSTRIAL CROPS AND PRODUCTS 191.B(2023):115775.

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