Comparative analysis of drought-responsive biochemical and transcriptomic mechanisms in two Dendrobium officinale genotypes

doi:10.1016/j.indcrop.2023.116766

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	Comparative analysis of drought-responsive biochemical and transcriptomic mechanisms in two Dendrobium officinale genotypes
	Huang,Hui; Jiao,Yixue; Tong,Yan; Wang,Yuhua
	2023
发表期刊	INDUSTRIAL CROPS AND PRODUCTS
ISSN	1872-633X
卷号	199 页码:116766
摘要	Drought stress is one of the most sever natural disaster, threatening to plant growth and global crop security. Dendrobium officinale is a drought-tolerance crop with effective defense mechanisms against drought stress, as well as an important economic plant for medicinal, cosmetic, or ornamental purposes. Revealing the regulatory mechanisms conferring drought resistance upon D. officinale is thus crucial for genetic breeding and water-saving agriculture. In this study, the biochemical and transcriptomic profiles of two D. officinale genotypes were comprehensively analyzed under three drought stress conditions. The M genotype has a relative weaker drought tolerance, as shown by withered leaves, rapidly accumulated malondialdehyde, and severely repressed expression of photosynthesis-related genes under water deficit. In the O genotype (drought-tolerant genotype), proline content, ascorbate peroxidase and catalase activities significantly increased with intensifying drought stress, showing a higher level than that in the M genotype, especially under severe drought stress. By contrast, superoxide dismutase and peroxidase activities were higher in the M genotype under moderate and severe water deficient. Transcriptome analysis demonstrated that mild water deficit initiated the plant hormone signal transduction pathway, while severe drought stress launched the flavonoid biosynthesis pathway in both D. officinale genotypes. Notably, high expression of most protein phosphatases type 2 C in the M genotype, a negative regulator of abscisic acid signaling, may partially explain the relative weaker drought tolerance of the M genotype. Moreover, the higher flavonoid content corresponding with the highly expressed PAL and DFR in the O genotype than in the M genotype, may confer a stronger drought tolerance upon the O genotype under water deficit. Additionally, the biased expression pattern of heat shock proteins, late embryogenesis abundant proteins, and dehydrins may also be linked to the different drought responses of the two D. officinale genotypes. Our results provide a theoretical basis for drought-tolerant crops breeding and water-sparing agriculture.
关键词	Dendrobium officinale Drought response Antioxidant enzymes Transcriptomic analysis Metabolites ABSCISIC-ACID STRESS TOLERANCE PHYSIOLOGICAL-RESPONSES SIGNAL-TRANSDUCTION FUNCTIONAL-ANALYSIS ENZYME-ACTIVITIES IMPROVES DROUGHT OXIDATIVE STRESS OVER-EXPRESSION DEHYDRIN GENE
学科领域	Agriculture
DOI	10.1016/j.indcrop.2023.116766
收录类别	SCI
WOS记录号	WOS:000988601400001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75568
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Huang,Hui,Jiao,Yixue,Tong,Yan,et al. Comparative analysis of drought-responsive biochemical and transcriptomic mechanisms in two Dendrobium officinale genotypes[J]. INDUSTRIAL CROPS AND PRODUCTS,2023,199:116766.
APA	Huang,Hui,Jiao,Yixue,Tong,Yan,&Wang,Yuhua.(2023).Comparative analysis of drought-responsive biochemical and transcriptomic mechanisms in two Dendrobium officinale genotypes.INDUSTRIAL CROPS AND PRODUCTS,199,116766.
MLA	Huang,Hui,et al."Comparative analysis of drought-responsive biochemical and transcriptomic mechanisms in two Dendrobium officinale genotypes".INDUSTRIAL CROPS AND PRODUCTS 199(2023):116766.

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