A new sesquiterpene synthase catalyzing the formation of (R)-fi-bisabolene from medicinal plant Colquhounia coccinea var. mollis and its anti-adipogenic and antibacterial activities

doi:10.1016/j.phytochem.2023.113681

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	A new sesquiterpene synthase catalyzing the formation of (R)-fi-bisabolene from medicinal plant Colquhounia coccinea var. mollis and its anti-adipogenic and antibacterial activities
	Li,De-Sen; Shi,Lin-Lin; Guo,Kai; Luo,Shi-Hong; Liu,Yan-Chun; Chen,Yue-Gui; Liu,Yan; Li,Sheng-Hong
	2023
发表期刊	PHYTOCHEMISTRY
ISSN	1873-3700
卷号	211 页码:113681
摘要	The sesquiterpene fi-bisabolene possessing R and S configurations is commonly found in plant essential oils with antimicrobial and antioxidant activities. Here, we report the cloning and functional characterization of a (R)-fi-bisabolene synthase gene (CcTPS2) from a Lamiaceae medicinal plant Colquhounia coccinea var. mollis. The biochemical function of CcTPS2 catalyzing the cyclization of farnesyl diphosphate to form a single product (R)-fi-bisabolene was characterized through an engineered Escherichia coli producing diverse polyprenyl diphosphate precursors and in vitro enzyme assay, indicating that CcTPS2 was a high-fidelity (R)-fi-bisabolene synthase. The production of (R)-fi-bisabolene in an engineered E. coli strain harboring the exogenous mevalonate pathway, farnesyl diphosphate synthase and CcTPS1 genes was 17 mg/L under shaking flask conditions. Ultimately, 120 mg of purified (R)-fi-bisabolene was obtained from the engineered E. coli, and its structure was elucidated by detailed spectroscopic analyses (including 1D and 2D NMR, and specific rotation). Four chimeric enzymes were constructed through domain swapping, which altered the product outcome, indicating the region important for substrate and product specificity. In addition, (R)-fi-bisabolene exhibited anti-adipogenic activity in the model organism Caenorhabditis elegans and antibacterial activity selectively against Gram-positive bacteria.
关键词	Sesquiterpenoids Sesquiterpene synthases (R)-fi-bisabolene Colquhounia coccinea var mollis Anti-adipogenic activity GERANYLFARNESYL DIPHOSPHATE SYNTHASE GLANDULAR TRICHOMES FUNCTIONAL EXPRESSION BETA-BISABOLENE ESSENTIAL OILS BIOSYNTHESIS IDENTIFICATION CYTOTOXICITY CLONING
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1016/j.phytochem.2023.113681
收录类别	SCI
WOS记录号	WOS:000988873400001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75529
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Li,De-Sen,Shi,Lin-Lin,Guo,Kai,et al. A new sesquiterpene synthase catalyzing the formation of (R)-fi-bisabolene from medicinal plant Colquhounia coccinea var. mollis and its anti-adipogenic and antibacterial activities[J]. PHYTOCHEMISTRY,2023,211:113681.
APA	Li,De-Sen.,Shi,Lin-Lin.,Guo,Kai.,Luo,Shi-Hong.,Liu,Yan-Chun.,...&Li,Sheng-Hong.(2023).A new sesquiterpene synthase catalyzing the formation of (R)-fi-bisabolene from medicinal plant Colquhounia coccinea var. mollis and its anti-adipogenic and antibacterial activities.PHYTOCHEMISTRY,211,113681.
MLA	Li,De-Sen,et al."A new sesquiterpene synthase catalyzing the formation of (R)-fi-bisabolene from medicinal plant Colquhounia coccinea var. mollis and its anti-adipogenic and antibacterial activities".PHYTOCHEMISTRY 211(2023):113681.

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