Characterization of two putative norlaudanosoline methyltransferases from Aristolochia debilis

doi:10.1016/j.jplph.2023.153983

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	Characterization of two putative norlaudanosoline methyltransferases from Aristolochia debilis
	Tuo,Xiaotao; Yu,Zhiyin; Li,Junheng; Qi,Yuxin; Peng,Guoqing; Huang,Sheng-Xiong; Huang,Xueshuang; Huang,Jian-Ping
	2023
发表期刊	JOURNAL OF PLANT PHYSIOLOGY
ISSN	1618-1328
卷号	285 页码:153983
摘要	In view of the nephrotoxicity, hepatotoxicity, and carcinogenicity of aristolochic acids (AAs), the removal of AAs from plants becomes an urgent priority for ensuring the safety of Aristolochia herbal materials. In this study, based on the root-predominant distribution of aristolochic acid I (AAI) in Aristolochia debilis, transcriptome sequencing, in combination with phylogenetic analyses, and gene expression pattern analysis together provided five candidate genes for investigating AAI biosynthesis. Comprehensive in vitro and in vivo enzymatic assays revealed that Ab6OMT1 (6-O-methyltransferase) and AbNMT1 (N-methyltransferase) exhibit promiscuity in substrate recognition, and they could act in a cooperative fashion to achieve conversion of norlaudanosoline, a predicted intermediate in AAI biosynthetic route, into 3'-hydroxy-N-methylcoclaurine through two different methylation reaction sequences. These results shed light on the molecular basis for AAI biosynthesis in Aristo-lochia herbs. More importantly, Ab6OMT1 and AbNMT1 may be employed as targets for the metabolic engi-neering of AAI biosynthesis to produce AAs-free Aristolochia herbal materials.
关键词	Aristolochia Aristolochic acids Biosynthetic pathway Transcriptome Methyltransferase BENZYLISOQUINOLINE ALKALOID BIOSYNTHESIS RATE-LIMITING STEP NORCOCLAURINE 6-O-METHYLTRANSFERASE PURIFICATION
学科领域	Plant Sciences
DOI	10.1016/j.jplph.2023.153983
收录类别	SCI
WOS记录号	WOS:000992644700001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75466
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Tuo,Xiaotao,Yu,Zhiyin,Li,Junheng,et al. Characterization of two putative norlaudanosoline methyltransferases from Aristolochia debilis[J]. JOURNAL OF PLANT PHYSIOLOGY,2023,285:153983.
APA	Tuo,Xiaotao.,Yu,Zhiyin.,Li,Junheng.,Qi,Yuxin.,Peng,Guoqing.,...&Huang,Jian-Ping.(2023).Characterization of two putative norlaudanosoline methyltransferases from Aristolochia debilis.JOURNAL OF PLANT PHYSIOLOGY,285,153983.
MLA	Tuo,Xiaotao,et al."Characterization of two putative norlaudanosoline methyltransferases from Aristolochia debilis".JOURNAL OF PLANT PHYSIOLOGY 285(2023):153983.

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