Knowledge Management System of Kunming Institute of Botany,CAS
Bioassay-guided isolation of α-Glucosidase inhibitory constituents from Hypericum sampsonii | |
Tao,Linlan; Xu,Shuangyu; Zhang,Zizhen; Li,Yanan; Yang,Jue; Gu,Wei![]() ![]() | |
2023 | |
发表期刊 | CHINESE JOURNAL OF NATURAL MEDICINES
![]() |
ISSN | 1875-5364 |
卷号 | 21期号:6页码:443-453 |
摘要 | This study employed the alpha-glucosidase inhibitory activity model as an anti-diabetic assay and implemented a bioactivity-guided isolation strategy to identify novel natural compounds with potential therapeutic properties. Hypericum sampsonii was investigated, leading to the isolation of two highly modified seco-polycyclic polyprenylated acylphloroglucinols (PPAPs) (1 and 2), eight phenolic derivatives (3-10), and four terpene derivatives (11-14). The structures of compounds 1 and 2, featuring an unprecedented octahydro-2H-chromen-2-one ring system, were fully characterized using extensive spectroscopic data and quantum chemistry calculations. Six compounds (1, 5-7, 9, and 14) exhibited potential inhibitory effects against a-glucosidase, with IC50 values ranging from 0.050 +/- 0.0016 to 366.70 +/- 11.08 mu g center dot mL(-1). Notably, compound 5 (0.050 +/- 0.0016 mu g center dot mL(-1)) was identified as the most potential aglucosidase inhibitor, with an inhibitory effect about 6900 times stronger than the positive control, acarbose (IC50 = 346.63 +/- 15.65 mu g center dot mL(-1)). A docking study was conducted to predict molecular interactions between two compounds (1 and 5) and a-glucosidase, and the hypothetical biosynthetic pathways of the two unprecedented seco-PPAPs were proposed. |
关键词 | Hypericum sampsonii Chemical constituents Structure elucidation alpha-Glucosidase Molecular docking POLYCYCLIC POLYPRENYLATED ACYLPHLOROGLUCINOLS POTENT INHIBITORS SPECTROSCOPY DERIVATIVES CHEMISTRY AMYLASE ROOTS |
DOI | 10.1016/S1875-5364(23)60472-8 |
收录类别 | SCI |
WOS记录号 | WOS:001037045400001 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.kib.ac.cn/handle/151853/75449 |
专题 | 中国科学院昆明植物研究所 |
推荐引用方式 GB/T 7714 | Tao,Linlan,Xu,Shuangyu,Zhang,Zizhen,et al. Bioassay-guided isolation of α-Glucosidase inhibitory constituents from Hypericum sampsonii[J]. CHINESE JOURNAL OF NATURAL MEDICINES,2023,21(6):443-453. |
APA | Tao,Linlan.,Xu,Shuangyu.,Zhang,Zizhen.,Li,Yanan.,Yang,Jue.,...&Yuan,Chunmao.(2023).Bioassay-guided isolation of α-Glucosidase inhibitory constituents from Hypericum sampsonii.CHINESE JOURNAL OF NATURAL MEDICINES,21(6),443-453. |
MLA | Tao,Linlan,et al."Bioassay-guided isolation of α-Glucosidase inhibitory constituents from Hypericum sampsonii".CHINESE JOURNAL OF NATURAL MEDICINES 21.6(2023):443-453. |
条目包含的文件 | 下载所有文件 | |||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
1-s2.0-S187553642360(1033KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论