Targeted quantitative analysis of monoterpenoid indole alkaloids in Alstonia scholaris by ultra-high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry

doi:10.1002/jssc.202200843

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	Targeted quantitative analysis of monoterpenoid indole alkaloids in Alstonia scholaris by ultra-high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry
	Qin,Yan; He,Ying-Jie; Zhao,Yun-Li; Zhou,Zhong-Shun; Wang,Zhao-Jie; Zhu,Yan-Yan; Luo,Xiao-Dong
	2023
发表期刊	JOURNAL OF SEPARATION SCIENCE
ISSN	1615-9314
摘要	Monoterpene indole alkaloids exhibit structural diversity in herbal resources and have been developed as promising drugs owing to their significant biological activities. Confidential identification and quantification of monoterpene indole alkaloids is the key to quality control of target plants in industrial production but has rarely been reported. In this study, quantitative performance of three data acquisition modes of ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry including full scan, auto-MS2 and target-MS2, was evaluated and compared for specificity, sensitivity, linearity, precision, accuracy, and matrix effect using five monoterpene indole alkaloids (scholaricine, 19-epi-scholaricine, vallesamine, picrinine, and picralinal). Method validations indicated that target-MS2 mode showed predominant performance for simultaneous annotation and quantification of analytes, and was then applied to determine monoterpene indole alkaloids in Alstonia scholaris (leaves, barks) after extraction procedures optimization using Box-Behnken design of response surface methodology. The variations of A. scholaris monoterpene indole alkaloids in different plant parts, harvest periods, and post-handling processes, were subsequently investigated. The results indicated that target-MS2 mode could improve the quantitative capability of ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry for structure-complex monoterpene indole alkaloids in herbal matrices.Alstonia scholaris, monoterpene indole alkaloids, quadrupole time of flight mass spectrometry, qualitative and quantitative analysis, ultra-high-performance liquid chromatography
关键词	CATHARANTHUS-ROSEUS ETHANOLIC EXTRACTS IDENTIFICATION QUANTIFICATION HPTLC
学科领域	Chemistry
DOI	10.1002/jssc.202200843
收录类别	SCI
WOS记录号	WOS:001014416200001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75441
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Qin,Yan,He,Ying-Jie,Zhao,Yun-Li,et al. Targeted quantitative analysis of monoterpenoid indole alkaloids in Alstonia scholaris by ultra-high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry[J]. JOURNAL OF SEPARATION SCIENCE,2023.
APA	Qin,Yan.,He,Ying-Jie.,Zhao,Yun-Li.,Zhou,Zhong-Shun.,Wang,Zhao-Jie.,...&Luo,Xiao-Dong.(2023).Targeted quantitative analysis of monoterpenoid indole alkaloids in Alstonia scholaris by ultra-high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry.JOURNAL OF SEPARATION SCIENCE.
MLA	Qin,Yan,et al."Targeted quantitative analysis of monoterpenoid indole alkaloids in Alstonia scholaris by ultra-high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry".JOURNAL OF SEPARATION SCIENCE (2023).

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