A Multiplex PCR System of Novel Microsatellite Loci for Population Genetic Application in Walnuts

doi:10.3390/plants12244101

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	A Multiplex PCR System of Novel Microsatellite Loci for Population Genetic Application in Walnuts
	Xiahou,Zuo-Ying; Wambulwa,Moses C.; Xu,Zu-Chang; Ye,Lin-Jiang; Fan,Peng-Zhen; Magige,Ephie A.; Luo,Ya-Huang; Liu,Jie
	2023
发表期刊	PLANTS-BASEL
ISSN	2223-7747
卷号	12 期号:24 页码:4101
摘要	Multiplex polymerase chain reaction (PCR) of microsatellite loci allows for simultaneous amplification of two or more pairs of primers in a single PCR reaction; hence, it is cost and time effective. However, very few attempts have been reported in non-model species. In this study, by combining a genome-based de novo development and cross-species application approach, a multiplex PCR system comprising 5 PCR reactions of 33 microsatellites consisting of 26 novel genomic and 7 literature-sourced loci was tested for polymorphisms, cross-species transferability, and the ability to assess genetic diversity and population structure of three walnut species (Juglans spp.). We found that the genome-based approach is more efficient than other methods. An allelic ladder was developed for each locus to enhance consistent genotyping among laboratories. The population genetic analysis results showed that all 33 loci were successfully transferred across the three species, showing high polymorphism and a strong genetic structure. Hence, the multiplex PCR system is highly applicable in walnut species. Furthermore, we propose an efficient pipeline to characterize and genotype polymorphic microsatellite loci. The novel toolbox developed here will aid future ecology and evolution studies in walnut and could serve as a model for other plant species.
关键词	genetic diversity Juglans microsatellite marker multiplex PCR transferability walnut CROSS-SPECIES AMPLIFICATION SSR-MARKERS JUGLANS-MANDSHURICA COMPUTER-PROGRAM DIVERSITY SOFTWARE SUCCESS ASSAY
学科领域	199
DOI	10.3390/plants12244101
WOS记录号	WOS:001136192300001
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75055
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Xiahou,Zuo-Ying,Wambulwa,Moses C.,Xu,Zu-Chang,et al. A Multiplex PCR System of Novel Microsatellite Loci for Population Genetic Application in Walnuts[J]. PLANTS-BASEL,2023,12(24):4101.
APA	Xiahou,Zuo-Ying.,Wambulwa,Moses C..,Xu,Zu-Chang.,Ye,Lin-Jiang.,Fan,Peng-Zhen.,...&Liu,Jie.(2023).A Multiplex PCR System of Novel Microsatellite Loci for Population Genetic Application in Walnuts.PLANTS-BASEL,12(24),4101.
MLA	Xiahou,Zuo-Ying,et al."A Multiplex PCR System of Novel Microsatellite Loci for Population Genetic Application in Walnuts".PLANTS-BASEL 12.24(2023):4101.

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