Structural Characterization and Anticoagulant Activities of a Keratan Sulfate-like Polysaccharide from the Sea Cucumber Holothuria fuscopunctata

doi:10.3390/md21120632

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	Structural Characterization and Anticoagulant Activities of a Keratan Sulfate-like Polysaccharide from the Sea Cucumber Holothuria fuscopunctata
	Chen,Ru; Wang,Weili; Yin,Ronghua; Pan,Ying; Xu,Chen; Gao,Na; Luo,Xiaodong; Zhao,Jinhua; Reyes,Fernando
	2023
发表期刊	MARINE DRUGS
ISSN	1660-3397
卷号	21 期号:12 页码:632
摘要	A sulfated polysaccharide (AG) was extracted and isolated from the sea cucumber H. fuscopunctata, consisting of GlcNAc, GalNAc, Gal, Fuc and lacking any uronic acid residues. Importantly, several chemical depolymerization methods were used to elucidate the structure of the AG through a bottom-up strategy. A highly sulfated galactose (oAG-1) and two disaccharides labeled with 2,5-anhydro-D-mannose (oAG-2, oAG-3) were obtained from the deaminative depolymerized product along with the structures of the disaccharide derivatives (oAG-4 similar to oAG-6) identified from the free radical depolymerized product, suggesting that the repeating building blocks in a natural AG should comprise the disaccharide beta-D-GalS-1,4-D-GlcNAc(6S). The possible disaccharide side chains (bAG-1) were obtained with mild acid hydrolysis. Thus, a natural AG may consist of a keratan sulfate-like (KS-like) glycosaminoglycan with diverse modifications, including the sulfation types of the Gal residue and the possible disaccharide branches alpha-D-GalNAc(4S6S)-1,2-alpha/beta-L-Fuc(3S) linked to the KS-like chain. Additionally, the anticoagulant activities of the AG and its depolymerized products (dAG1-9) were evaluated in vitro using normal human plasma. The AG could prolong activated partial thromboplastin time (APTT) in a dose-dependent manner, and the activity potency was positively related to the chain length. The AG and dAG1-dAG3 could prolong thrombin time (TT), while they had little effect on prothrombin time (PT). The results indicate that the AG could inhibit the intrinsic and common coagulation pathways.
关键词	aminoglycan structural features keratan sulfate depolymerization oligosaccharides anticoagulant activity INTRINSIC-FACTOR XASE GLYCOSAMINOGLYCAN OLIGOSACCHARIDES DEPOLYMERIZATION ELUCIDATION DERIVATIVES HEPARIN
学科领域	376
DOI	10.3390/md21120632
WOS记录号	WOS:001132314500001
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75032
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Chen,Ru,Wang,Weili,Yin,Ronghua,et al. Structural Characterization and Anticoagulant Activities of a Keratan Sulfate-like Polysaccharide from the Sea Cucumber Holothuria fuscopunctata[J]. MARINE DRUGS,2023,21(12):632.
APA	Chen,Ru.,Wang,Weili.,Yin,Ronghua.,Pan,Ying.,Xu,Chen.,...&Reyes,Fernando.(2023).Structural Characterization and Anticoagulant Activities of a Keratan Sulfate-like Polysaccharide from the Sea Cucumber Holothuria fuscopunctata.MARINE DRUGS,21(12),632.
MLA	Chen,Ru,et al."Structural Characterization and Anticoagulant Activities of a Keratan Sulfate-like Polysaccharide from the Sea Cucumber Holothuria fuscopunctata".MARINE DRUGS 21.12(2023):632.

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