S-nitrosylation of SlAPX Is Involved in Alleviating Oxidative Damage in Transgenic Tobacco under Nitrate Stress

doi:10.3390/agronomy13051322

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	S-nitrosylation of SlAPX Is Involved in Alleviating Oxidative Damage in Transgenic Tobacco under Nitrate Stress
	Lv,Chuntao; Liang,Yuanlin; Wang,Manqi; Li,Kunzhi; Sun,Xudong; Xu,Huini
	2023
发表期刊	AGRONOMY-BASEL
ISSN	2073-4395
卷号	13 期号:5 页码:1322
摘要	Nitric oxide (NO) modulates plant response by post-translationally modifying proteins, mainly through S-nitrosylation. Ascorbate peroxidase (APX) in the ascorbate-glutathione (AsA-GSH) cycle participates in the removal of hydrogen peroxide (H2O2). However, the relationship between S-nitrosylation and the role of tomato APX (SlAPX) under nitrate stress is still unclear. In this study, the enzyme activity, mRNA expression, and S-nitrosylation level of SlAPX were significantly increased in tomato roots after nitrate treatment. SlAPX protein could be S-nitrosylated by S-nitrosoglutathione in vitro, and APX activity was significantly increased after S-nitrosylation. The SlAPX overexpressed tobacco plants grew better than the wild type (WT) plants under nitrate stress. Meanwhile, the transgenic plants showed lower reactive oxygen species and malondialdehyde content, higher APX, monodehydroascorbate reductase, glutathione reductase activities, ascorbic acid/dehydroascorbic acid, and reduced glutathione/oxidized glutathione ratio, proline, and soluble sugar contents than those in the WT plants under nitrate treatment. Moreover, overexpressed transgenic seeds showed higher tolerance to methyl viologen induced oxidative stress compared with the WT. The NO accumulation and S-nitrosylation APX level were higher in transgenic plants than in WT plants after nitrate stress treatment. Our results provide novel insights into the mechanism of SlAPX modulation excess nitrate stress tolerance involving the S-nitrosylation modification.
关键词	ascorbate peroxidase NO AsA-GSH cycle tomato CYTOSOLIC ASCORBATE PEROXIDASE NITRIC-OXIDE SUPEROXIDE-DISMUTASE NACL STRESS GLUTATHIONE-REDUCTASE TYROSINE NITRATION HYDROGEN-PEROXIDE ACTIVE OXYGEN SALT STRESS TOLERANCE
学科领域	Agriculture ; Plant Sciences
DOI	10.3390/agronomy13051322
WOS记录号	WOS:000994174400001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74992
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Lv,Chuntao,Liang,Yuanlin,Wang,Manqi,et al. S-nitrosylation of SlAPX Is Involved in Alleviating Oxidative Damage in Transgenic Tobacco under Nitrate Stress[J]. AGRONOMY-BASEL,2023,13(5):1322.
APA	Lv,Chuntao,Liang,Yuanlin,Wang,Manqi,Li,Kunzhi,Sun,Xudong,&Xu,Huini.(2023).S-nitrosylation of SlAPX Is Involved in Alleviating Oxidative Damage in Transgenic Tobacco under Nitrate Stress.AGRONOMY-BASEL,13(5),1322.
MLA	Lv,Chuntao,et al."S-nitrosylation of SlAPX Is Involved in Alleviating Oxidative Damage in Transgenic Tobacco under Nitrate Stress".AGRONOMY-BASEL 13.5(2023):1322.

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