Comparative analysis of the medicinal plant Polygonatum kingianum (Asparagaceae) with related verticillate leaf types of the Polygonatum species based on chloroplast genomes

doi:10.3389/fpls.2023.1202634

KIB OpenIR

	Comparative analysis of the medicinal plant Polygonatum kingianum (Asparagaceae) with related verticillate leaf types of the Polygonatum species based on chloroplast genomes
	Shi,Naixing; Yang,Zefen; Miao,Ke; Tang,Lilei; Zhou,Nian; Xie,Pingxuan; Wen,Guosong
	2023
发表期刊	FRONTIERS IN PLANT SCIENCE
卷号	14 页码:1202634
摘要	BackgroundPolygonatum kingianum has been widely used as a traditional Chinese medicine as well as a healthy food. Because of its highly variable morphology, this medicinal plant is often difficult to distinguish from other related verticillate leaf types of the Polygonatum species. The contaminants in P. kingianum products not only decrease the products' quality but also threaten consumer safety, seriously inhibiting the industrial application of P. kingianum.MethodsNine complete chloroplast (cp) genomes of six verticillate leaf types of the Polygonatum species were de novo assembled and systematically analyzed.ResultsThe total lengths of newly sequenced cp genomes ranged from 155,437 to 155,977 bp, including 86/87 protein-coding, 38 tRNA, and 8 rRNA genes, which all exhibited well-conserved genomic structures and gene orders. The differences in the IR/SC (inverted repeats/single-copy) boundary regions and simple sequence repeats were detected among the verticillate leaf types of the Polygonatum cp genomes. Comparative cp genomes analyses revealed that a higher similarity was conserved in the IR regions than in the SC regions. In addition, 11 divergent hotspot regions were selected, providing potential molecular markers for the identification of the Polygonatum species with verticillate leaf types. Phylogenetic analysis indicated that, as a super barcode, plastids realized a fast and efficient identification that clearly characterized the relationships within the verticillate leaf types of the Polygonatum species. In brief, our results not only enrich the data on the cp genomes of the genus Polygonatum but also provide references for the P. kingianum germplasm resource protection, herbal cultivation, and drug production.ConclusionThis study not only accurately identifies P. kingianum species, but also provides valuable information for the development of molecular markers and phylogenetic analyses of the Polygonatum species with verticillate leaf types.
关键词	Polygonatum verticillate leaf Polygonatum kingianum chloroplast genome comparative analysis phylogenetic analysis CODON USAGE GENUS POLYGONATUM SOFTWARE TRANSFERABILITY MITOCHONDRIAL MARKERS MODEL
DOI	10.3389/fpls.2023.1202634
WOS记录号	WOS:001062926100001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74984
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Shi,Naixing,Yang,Zefen,Miao,Ke,et al. Comparative analysis of the medicinal plant Polygonatum kingianum (Asparagaceae) with related verticillate leaf types of the Polygonatum species based on chloroplast genomes[J]. FRONTIERS IN PLANT SCIENCE,2023,14:1202634.
APA	Shi,Naixing.,Yang,Zefen.,Miao,Ke.,Tang,Lilei.,Zhou,Nian.,...&Wen,Guosong.(2023).Comparative analysis of the medicinal plant Polygonatum kingianum (Asparagaceae) with related verticillate leaf types of the Polygonatum species based on chloroplast genomes.FRONTIERS IN PLANT SCIENCE,14,1202634.
MLA	Shi,Naixing,et al."Comparative analysis of the medicinal plant Polygonatum kingianum (Asparagaceae) with related verticillate leaf types of the Polygonatum species based on chloroplast genomes".FRONTIERS IN PLANT SCIENCE 14(2023):1202634.

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