Chromosome-level genome assembly of Murraya paniculata sheds light on biosynthesis of floral volatiles

doi:10.1186/s12915-023-01639-6

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	Chromosome-level genome assembly of Murraya paniculata sheds light on biosynthesis of floral volatiles
	Yang,Tianyu; Yin,Xin; Kang,Haotong; Yang,Danni; Yang,Xingyu; Yang,Yunqiang; Yang,Yongping
	2023
发表期刊	BMC BIOLOGY
ISSN	1741-7007
卷号	21 期号:1 页码:142
摘要	BackgroundMurraya paniculata (L.) Jack, commonly called orange jessamine in the family Rutaceae, is an important ornamental plant in tropical and subtropical regions which is famous for its strong fragrance. Although genome assemblies have been reported for many Rutaceae species, mainly in the genus Citrus, full genomic information has not been reported for M. paniculata, which is a prerequisite for in-depth genetic studies on Murraya and manipulation using genetic engineering techniques. Here, we report a high-quality chromosome-level genome assembly of M. paniculata and aim to provide insights on the molecular mechanisms of flower volatile biosynthesis.ResultsThe genome assembly with a contig N50 of 18.25 Mb consists of 9 pseudomolecules and has a total length of 216.86 Mb. Phylogenetic analysis revealed that M. paniculata diverged from the common ancestor approximately 25 million years ago and has not undergone any species-specific whole genome duplication events. Genome structural annotation and comparative genomics analysis revealed that there are obvious differences in transposon contents among the genomes of M. paniculata and Citrus species, especially in the upstream regions of genes. Research on the flower volatiles of M. paniculata and C. maxima at three flowering stages revealed significant differences in volatile composition with the flowers of C. maxima lacking benzaldehyde and phenylacetaldehyde. Notably, there are transposons inserted in the upstream region of the phenylacetaldehyde synthase (PAAS) genes Cg1g029630 and Cg1g029640 in C. maxima, but not in the upstream region of three PAAS genes Me2G_2379, Me2G_2381, and Me2G_2382 in M. paniculata. Our results indicated that compared to the low expression levels of PAAS genes in C. maxima, the higher expression levels of the three PAAS genes in M. paniculata are the main factor affecting the phenylacetaldehyde biosynthesis and causing the content difference of phenylacetaldehyde. The phenylacetaldehyde synthetic activities of the enzymes encoded by M. paniculata PAAS genes were validated by in vitro analyses.ConclusionsOur study provides useful genomic resources of M. paniculata for further research on Rutaceae plants, identifies new PAAS genes, and provides insights into how transposons contribute to variations in flower volatiles among Murraya and Citrus plants.
关键词	Murraya paniculata Citrus species Comparative genomics Transposon Flower volatiles Phenylacetaldehyde CITRUS SYSTEM RETROTRANSPOSONS IDENTIFICATION EVOLUTION ALIGNMENT PROVIDES
学科领域	Life Sciences & Biomedicine - Other Topics
DOI	10.1186/s12915-023-01639-6
WOS记录号	WOS:001011864200002
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74953
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Yang,Tianyu,Yin,Xin,Kang,Haotong,et al. Chromosome-level genome assembly of Murraya paniculata sheds light on biosynthesis of floral volatiles[J]. BMC BIOLOGY,2023,21(1):142.
APA	Yang,Tianyu.,Yin,Xin.,Kang,Haotong.,Yang,Danni.,Yang,Xingyu.,...&Yang,Yongping.(2023).Chromosome-level genome assembly of Murraya paniculata sheds light on biosynthesis of floral volatiles.BMC BIOLOGY,21(1),142.
MLA	Yang,Tianyu,et al."Chromosome-level genome assembly of Murraya paniculata sheds light on biosynthesis of floral volatiles".BMC BIOLOGY 21.1(2023):142.

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