Comparative transcriptomes reveal the disjunction adaptive strategy of Thuja species in East Asia and North America

doi:10.1007/s11676-023-01648-9

KIB OpenIR

	Comparative transcriptomes reveal the disjunction adaptive strategy of Thuja species in East Asia and North America
	Chang,Ermei; Liu,Xue; Chen,Jiahui; Sun,Jingyi; Yang,Shaowei; Liu,Jianfeng
	2023
发表期刊	JOURNAL OF FORESTRY RESEARCH
ISSN	1993-0607
卷号	34 期号:6 页码:1963-1974
摘要	The genus Thuja is ideal for investigating the genetic basis of the East Asia-North America disjunction. The biogeographical background of the genus is debatable and an adaptive strategy is lacking. Through the analysis and mining of comparative transcriptomes, species differentiation and positively selected genes (PSGs) were identified to provide information for understanding the environmental adaptation strategies of the genus Thuja. De novo assembly yielded 44,397-74,252 unigenes of the five Thuja species with contig N-50 length ranging from 1,559 to 1,724 bp. Annotations revealed a similar distribution of functional categories among them. Based on the phylogenetic trees constructed using the transcriptome data, T. sutchuenensis was divided first, followed by T. plicata and T. occidentalis. The final differentiation of T. koraiensis and T. standishii formed a clade. Enrichment analysis indicated that the PSGs of the North American Thuja species were involved in plant hormone signal transduction and carbon fixation of photosynthetic organisms pathways. The PSGs of East Asian Thuja were related to phenolic, alkaloid, and terpenoid synthesis, important stress-resistant genes and could increase plant resistance to external environmental stresses. This study discovered numerous aroma synthetic-related PSGs including terpene synthase (TPS) genes and lipid phosphate phosphatase 2 (LPP2), associated with the synthetic aroma of T. sutchuenensis. Physiological indicators, such as the contents of soluble sugars, total chlorophyll, total phenolics, and total flavonoids were determined, which are consistent with the PSGs enrichment pathways associated with adaptive strategies in the five Thuja species. The results of this study provide an important basis for future studies on conservation genetics.
关键词	Thuja species Comparative transcriptomes East Asia-North America disjunction Specific gene Positively selected gene FLAVONOID BIOSYNTHESIS PLANT SUTCHUENENSIS DIVERSITY MARKERS
DOI	10.1007/s11676-023-01648-9
WOS记录号	WOS:001066951500001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74827
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Chang,Ermei,Liu,Xue,Chen,Jiahui,et al. Comparative transcriptomes reveal the disjunction adaptive strategy of Thuja species in East Asia and North America[J]. JOURNAL OF FORESTRY RESEARCH,2023,34(6):1963-1974.
APA	Chang,Ermei,Liu,Xue,Chen,Jiahui,Sun,Jingyi,Yang,Shaowei,&Liu,Jianfeng.(2023).Comparative transcriptomes reveal the disjunction adaptive strategy of Thuja species in East Asia and North America.JOURNAL OF FORESTRY RESEARCH,34(6),1963-1974.
MLA	Chang,Ermei,et al."Comparative transcriptomes reveal the disjunction adaptive strategy of Thuja species in East Asia and North America".JOURNAL OF FORESTRY RESEARCH 34.6(2023):1963-1974.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
10.1007_s11676-023-0（1095KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览下载