Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa

doi:10.1186/s13007-022-00931-w

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	Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa
	Liu, Yuanyuan; Zhang, Li; Li, Cheng; Yang, Yunqiang; Duan, Yuanwen; Yang, Yongping; Sun, Xudong
	2022
发表期刊	PLANT METHODS
卷号	18 期号:1 页码:98
摘要	Background Genome editing is essential for crop molecular breeding. However, gene editing in turnip (Brassica rapa var. rapa) have not been reported owing to the very low transformation efficiency. Results In this study, we established a transformation procedure involving chemical-inducible activation of the BrrWUSa gene, which resulted in high transformation frequencies of turnip. Estradiol-inducible BrrWUSa transgenic plants were fertile and showed no obvious developmental defects. Furthermore, we used CRISPR/Cas9 gene-editing technology to edit BrrTCP4b and generated 20 BrrTCP4b-edited seedlings with an increase in leaf trichome number. Conclusion The results demonstrate that BrrWUSa improves the regeneration efficiency in turnip. The transformation procedure represents a promising strategy to improve genetic transformation and for functional characterization of genes in turnip.
关键词	Turnip Brassica rapa var rapa BrrWUSa Genetic transformation Genome editing EXPRESSION
DOI	10.1186/s13007-022-00931-w
收录类别	SCI
WOS记录号	WOS:000836797200001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74787
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Liu, Yuanyuan,Zhang, Li,Li, Cheng,et al. Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa[J]. PLANT METHODS,2022,18(1):98.
APA	Liu, Yuanyuan.,Zhang, Li.,Li, Cheng.,Yang, Yunqiang.,Duan, Yuanwen.,...&Sun, Xudong.(2022).Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa.PLANT METHODS,18(1),98.
MLA	Liu, Yuanyuan,et al."Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa".PLANT METHODS 18.1(2022):98.

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