A new polysaccharide platform constructs self-adjuvant nanovaccines to enhance immune responses

doi:10.1186/s12951-022-01533-3

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	A new polysaccharide platform constructs self-adjuvant nanovaccines to enhance immune responses
	Chen, Sisi; Yang, Liu; Ou, Xia; Li, Jin-Yu; Zi, Cheng-Ting; Wang, Hao; Hu, Jiang-Miao; Liu, Ye
	2022
发表期刊	JOURNAL OF NANOBIOTECHNOLOGY
卷号	20 期号:1 页码:320
摘要	Background: Nanovaccines have shown the promising potential in controlling and eradicating the threat of infectious diseases worldwide. There has been a great need in developing a versatile strategy to conveniently construct diverse types of nanovaccines and induce potent immune responses. To that end, it is critical for obtaining a potent self-adjuvant platform to assemble with different types of antigens into nanovaccines. Results: In this study, we identified a new natural polysaccharide from the rhizomes of Bletilla striata (PRBS), and used this polysaccharide as a platform to construct diverse types of nanovaccines with potent self-adjuvant property. In the construction process of SARS-CoV-2 nanovaccine, PRBS molecules and RBD protein antigens were assembled into similar to 300 nm nanoparticles by hydrogen bond. For HIV nanovaccine, hydrophobic effect dominantly drove the co-assembly between PRBS molecules and Env expression plasmid into similar to 350 nm nanospheres. Importantly, PRBS can potently activate the behaviors and functions of multiple immune cells such as macrophages, B cells and dendritic cells. Depending on PRBS-mediated immune activation, these self-adjuvant nanovaccines can elicit significantly stronger antigen-specific antibody and cellular responses in vivo, in comparison with their corresponding traditional vaccine forms. Moreover, we also revealed the construction models of PRBS-based nanovaccines by analyzing multiple assembly parameters such as bond energy, bond length and interaction sites. Conclusions: PRBS, a newly-identified natural polysaccharide which can co-assemble with different types of antigens and activate multiple critical immune cells, has presented a great potential as a versatile platform to develop potent self-adjuvant nanovaccines.
关键词	Polysaccharide Self-adjuvant Nanovaccine SARS-CoV-2 HIV STRUCTURAL-CHARACTERIZATION DENDRITIC CELLS DRUG-DELIVERY NANOPARTICLES PARTICLES CHITOSAN GLYCOGEN DESIGN
DOI	10.1186/s12951-022-01533-3
收录类别	SCI
WOS记录号	WOS:000825479500005
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74771
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Chen, Sisi,Yang, Liu,Ou, Xia,et al. A new polysaccharide platform constructs self-adjuvant nanovaccines to enhance immune responses[J]. JOURNAL OF NANOBIOTECHNOLOGY,2022,20(1):320.
APA	Chen, Sisi.,Yang, Liu.,Ou, Xia.,Li, Jin-Yu.,Zi, Cheng-Ting.,...&Liu, Ye.(2022).A new polysaccharide platform constructs self-adjuvant nanovaccines to enhance immune responses.JOURNAL OF NANOBIOTECHNOLOGY,20(1),320.
MLA	Chen, Sisi,et al."A new polysaccharide platform constructs self-adjuvant nanovaccines to enhance immune responses".JOURNAL OF NANOBIOTECHNOLOGY 20.1(2022):320.

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