Developing Penicillium digitatum Management Strategies on Post-Harvest Citrus Fruits with Metabolic Components and Colonization of Bacillus subtilis L1-21

doi:10.3390/jof8010080

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	Developing Penicillium digitatum Management Strategies on Post-Harvest Citrus Fruits with Metabolic Components and Colonization of Bacillus subtilis L1-21
	Li, Yongmei; Xia, Mengyuan; He, Pengbo; Yang, Qiaoming; Wu, Yixin; He, Pengfei; Ahmed, Ayesha; Li, Xiangsong; Wang, Yuehu; Munir, Shahzad; He, Yueqiu
	2022
发表期刊	JOURNAL OF FUNGI
卷号	8 期号:1 页码:80
摘要	Citrus is among the most important plants in the fruit industry severely infected with pathogens. Citrus green mold caused by Penicillium digitatum is one of the most devastating diseases during post-harvest stages of citrus fruit. In this study, a potential endophyte Bacillus subtilis L1-21, isolated from healthy citrus plants, was assessed for its biocontrol activity against the pathogen P. digitatum. Based on an in vitro crosstalk assay, we suggested that B. subtilis L1-21 inhibits the pathogen with an inhibition zone of 3.51 +/- 0.08 cm. Biocontrol efficacy was highest for the fermented culture filtrate of B. subtilis L1-21. Additionally, using GC-MS analysis, 13 compounds were detected in the extract of this endophyte. The culture filtrate in Landy medium could enlarge and deform pathogen spores and prevent them from developing into normal mycelium. Accordingly, the Landy culture filtrate of B. subtilis L1-21 was stable in the temperature range of 4-90 degrees C and pH of 3-11. Further, MALDI-TOF-MS for B. subtilis L1-21 detected surfactin, fengycin, bacillaene and bacilysin as potential antifungal compounds. GFP-tagged B. subtilis L1-21 easily colonized in citrus fruit peel and pulp, suggesting its role in eliminating the fungal pathogen. Altogether, it is highly expected that the production of antifungal compounds, and the colonization potential of B. subtilis L1-21 are required against the post-harvest P. digitatum pathogen on citrus fruit.
关键词	fungi pathogen Penicillium digitatum Bacillus subtilis L1-21 LC-MS GREEN MOLD VOLATILE COMPOUNDS STRAINS GROWTH SACC. DECAY
DOI	10.3390/jof8010080
收录类别	SCI
WOS记录号	WOS:000758781500001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74743
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Li, Yongmei,Xia, Mengyuan,He, Pengbo,et al. Developing Penicillium digitatum Management Strategies on Post-Harvest Citrus Fruits with Metabolic Components and Colonization of Bacillus subtilis L1-21[J]. JOURNAL OF FUNGI,2022,8(1):80.
APA	Li, Yongmei.,Xia, Mengyuan.,He, Pengbo.,Yang, Qiaoming.,Wu, Yixin.,...&He, Yueqiu.(2022).Developing Penicillium digitatum Management Strategies on Post-Harvest Citrus Fruits with Metabolic Components and Colonization of Bacillus subtilis L1-21.JOURNAL OF FUNGI,8(1),80.
MLA	Li, Yongmei,et al."Developing Penicillium digitatum Management Strategies on Post-Harvest Citrus Fruits with Metabolic Components and Colonization of Bacillus subtilis L1-21".JOURNAL OF FUNGI 8.1(2022):80.

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