The Extracts of Dendrobium Alleviate Dry Eye Disease in Rat Model by Regulating Aquaporin Expression and MAPKs/NF-kappa B Signalling

doi:10.3390/ijms231911195

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	The Extracts of Dendrobium Alleviate Dry Eye Disease in Rat Model by Regulating Aquaporin Expression and MAPKs/NF-kappa B Signalling
	Ling, Jiawei; Chan, Chung-Lap; Ho, Chi-Yan; Gao, Xun; Tsang, Sin-Man; Leung, Ping-Chung; Hu, Jiang-Miao; Wong, Chun-Kwok
	2022
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	23 期号:19 页码:11195
摘要	Dry eye is one of the most common ocular surface diseases caused by tear film instability and ocular surface damage due to an abnormal quality or quantity of tears. Inflammatory factors can initiate relevant transduction signalling pathways and trigger the inflammatory cascade response, resulting in ocular surface inflammation. It has been shown that the active ingredients in Dendrobium, such as polysaccharides, alkaloids and phenols, have anti-inflammatory, anti-tumour and immunity-boosting effects, and Dendrobium officinale extract can improve glandular secretion function, increase salivary secretion and increase the expression level of water channel protein in salivary glands in patients with dry eye syndromes. We investigated the in vitro cytoprotective effect of Dendrobium extracts in sodium chloride induced hyperosmotic conditions in human cornea keratocytes (HKs). Results showed that Dendrobium officinale Kimura et Migo water extract (DOW) and Dendrobium loddigesii Rolfe water extract (DLW) could upregulate the expression of aquaporins (AQP)5 protein, thus exerting a repairing effect by promoting cell migration. Furthermore, oral administration of DOW and DLW enhanced tear production in rats and exerted a protective effect on ocular surface damage. DOW and DLW could upregulate the expression of AQP5 and mucin (muc)5ac proteins in the lacrimal gland and reduce the inflammatory response. DOW and DLW inhibited the activation of the corresponding mitogen-activated protein kinases (MAPK) and NF-KB pathway, thereby playing a role in improving dry eye symptoms. This study provides a new perspective on dry eye treatment, and DOW and DLW may be potential therapeutic agents for dry eye.
关键词	dry eye disease Dendrobium aquaporin scopolamine human cornea keratocytes TNF-ALPHA ACTIVATION CLASSIFICATION INFLAMMATION DEFINITION IL-1-BETA APOPTOSIS PATHWAYS STRESS MMP-9
DOI	10.3390/ijms231911195
收录类别	SCI
WOS记录号	WOS:000867779100001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74723
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Ling, Jiawei,Chan, Chung-Lap,Ho, Chi-Yan,et al. The Extracts of Dendrobium Alleviate Dry Eye Disease in Rat Model by Regulating Aquaporin Expression and MAPKs/NF-kappa B Signalling[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(19):11195.
APA	Ling, Jiawei.,Chan, Chung-Lap.,Ho, Chi-Yan.,Gao, Xun.,Tsang, Sin-Man.,...&Wong, Chun-Kwok.(2022).The Extracts of Dendrobium Alleviate Dry Eye Disease in Rat Model by Regulating Aquaporin Expression and MAPKs/NF-kappa B Signalling.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(19),11195.
MLA	Ling, Jiawei,et al."The Extracts of Dendrobium Alleviate Dry Eye Disease in Rat Model by Regulating Aquaporin Expression and MAPKs/NF-kappa B Signalling".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.19(2022):11195.

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