Cold Tolerance of ScCBL6 Is Associated with Tonoplast Transporters and Photosynthesis in Arabidopsis

doi:10.3390/cimb44110378

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	Cold Tolerance of ScCBL6 Is Associated with Tonoplast Transporters and Photosynthesis in Arabidopsis
	Zhou, Yanli; Zhang, Jingling; Zhao, Changhong; Long, Guangqiang; Zhou, Chengli; Sun, Xudong; Yang, Yunqiang; Zhang, Chengjun; Yang, Yongping
	2022
发表期刊	CURRENT ISSUES IN MOLECULAR BIOLOGY
卷号	44 期号:11 页码:5579-5592
摘要	Plants that are adapted to harsh environments offer enormous opportunity to understand stress responses in ecological systems. Stipa capillacea is widely distributed in the frigid and arid region of the Tibetan Plateau, but its signal transduction system under cold stress has not been characterized. In this study, we isolated a cDNA encoding the signal transduction protein, ScCBL6, from S. capillacea, and evaluated its role in cold tolerance by ectopically expressing it in Arabidopsis. Full-length ScCBL6 encode 227 amino acids, and are clustered with CBL6 in Stipa purpurea and Oryza sativa in a phylogenetic analysis. Compared with tolerance in wild-type (WT) plants, ScCBL6-overexpressing plants (ScCBL6-OXP) were more tolerant to cold stress but not to drought stress, as confirmed by their high photosynthetic capacity (Fv/Fm) and survival rate under cold stress. We further compared their cold-responsive transcriptome profiles by RNA sequencing. In total, 3931 genes were differentially expressed by the introduction of ScCBL6. These gene products were involved in multiple processes such as the immune system, lipid catabolism, and secondary metabolism. A KEGG pathway analysis revealed that they were mainly enriched in plant hormone signal transduction and biomacromolecule metabolism. Proteins encoded by differentially expressed genes were predicted to be localized in chloroplasts, mitochondria, and vacuoles, suggesting that ScCBL6 exerts a wide range of functions. Based on its tonoplast subcellular location combined with integrated transcriptome and physiological analyses of ScCBL6-OXP, we inferred that ScCBL6 improves plant cold stress tolerance in Arabidopsis via the regulation of photosynthesis, redox status, and tonoplast metabolite transporters.
关键词	calcineurin B-like protein cold tolerance Stipa capillacea transcriptome tonoplast CALCIUM SENSOR GENE-EXPRESSION MONOSACCHARIDE TRANSPORTER STRESS TOLERANCE ABIOTIC STRESS DROUGHT ACCLIMATION PROTEINS PHOSPHORYLATION OVEREXPRESSION
DOI	10.3390/cimb44110378
收录类别	SCI
WOS记录号	WOS:000883432600001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74699
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Zhou, Yanli,Zhang, Jingling,Zhao, Changhong,et al. Cold Tolerance of ScCBL6 Is Associated with Tonoplast Transporters and Photosynthesis in Arabidopsis[J]. CURRENT ISSUES IN MOLECULAR BIOLOGY,2022,44(11):5579-5592.
APA	Zhou, Yanli.,Zhang, Jingling.,Zhao, Changhong.,Long, Guangqiang.,Zhou, Chengli.,...&Yang, Yongping.(2022).Cold Tolerance of ScCBL6 Is Associated with Tonoplast Transporters and Photosynthesis in Arabidopsis.CURRENT ISSUES IN MOLECULAR BIOLOGY,44(11),5579-5592.
MLA	Zhou, Yanli,et al."Cold Tolerance of ScCBL6 Is Associated with Tonoplast Transporters and Photosynthesis in Arabidopsis".CURRENT ISSUES IN MOLECULAR BIOLOGY 44.11(2022):5579-5592.

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