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| Comparative Chloroplast Genome Analysis of Wax Gourd (Benincasa hispida) with Three Benincaseae Species, Revealing Evolutionary Dynamic Patterns and Phylogenetic Implications | |
| Song, Weicai; Chen, Zimeng; He, Li; Feng, Qi; Zhang, Hongrui; Du, Guilin; Shi, Chao; Wang, Shuo | |
| 2022 | |
| Source Publication | GENES
 |
| Volume | 13Issue:3Pages:461 |
| Abstract | Benincasa hispida (wax gourd) is an important Cucurbitaceae crop, with enormous economic and medicinal importance. Here, we report the de novo assembly and annotation of the complete chloroplast genome of wax gourd with 156,758 bp in total. The quadripartite structure of the chloroplast genome comprises a large single-copy (LSC) region with 86,538 bp and a small single-copy (SSC) region with 18,060 bp, separated by a pair of inverted repeats (IRa and IRb) with 26,080 bp each. Comparison analyses among B. hispida and three other species from Benincaseae presented a significant conversion regarding nucleotide content, genome structure, codon usage, synonymous and non-synonymous substitutions, putative RNA editing sites, microsatellites, and oligonucleotide repeats. The LSC and SSC regions were found to be much more varied than the IR regions through a divergent analysis of the species within Benincaseae. Notable IR contractions and expansions were observed, suggesting a difference in genome size, gene duplication and deletion, and the presence of pseudogenes. Intronic gene sequences, such as trnR-UCU-atpA and atpH-atpI, were observed as highly divergent regions. Two types of phylogenetic analysis based on the complete cp genome and 72 genes suggested sister relationships between B. hispida with the Citrullus, Lagenaria, and Cucumis. Variations and consistency with previous studies regarding phylogenetic relationships are discussed. The cp genome of B. hispida provides valuable genetic information for the detection of molecular markers, research on taxonomic discrepancies, and the inference of the phylogenetic relationships of Cucurbitaceae. |
| Keyword | Benincasa hispida chloroplast genome comparative analysis divergence region phylogenetic CUCURBITACEAE SEQUENCES PLANTS ANNOTATION EXTRACT FAMILY FRUITS GENES TOOLS |
| DOI | 10.3390/genes13030461 |
| Indexed By | SCI |
| WOS ID | WOS:000775289300001 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.kib.ac.cn/handle/151853/74689 |
| Collection | 中国科学院昆明植物研究所 |
| Recommended Citation GB/T 7714 | Song, Weicai,Chen, Zimeng,He, Li,et al. Comparative Chloroplast Genome Analysis of Wax Gourd (Benincasa hispida) with Three Benincaseae Species, Revealing Evolutionary Dynamic Patterns and Phylogenetic Implications[J]. GENES,2022,13(3):461. |
| APA | Song, Weicai.,Chen, Zimeng.,He, Li.,Feng, Qi.,Zhang, Hongrui.,...&Wang, Shuo.(2022).Comparative Chloroplast Genome Analysis of Wax Gourd (Benincasa hispida) with Three Benincaseae Species, Revealing Evolutionary Dynamic Patterns and Phylogenetic Implications.GENES,13(3),461. |
| MLA | Song, Weicai,et al."Comparative Chloroplast Genome Analysis of Wax Gourd (Benincasa hispida) with Three Benincaseae Species, Revealing Evolutionary Dynamic Patterns and Phylogenetic Implications".GENES 13.3(2022):461. |
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| File Name/Size | DocType | Version | Access | License | ||
| ARTN 461.pdf（1176KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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