油杉属(Keteleeria)植物外生菌根真菌多样性、群落组成及其影响因素研究

	油杉属(Keteleeria)植物外生菌根真菌多样性、群落组成及其影响因素研究; Diversity, community composition and driving factors of ectomycorrhizal fungi associated with Keteleeria
	许天修
导师	葛再伟
摘要	Ectomycorrhiza (ECM), a structure formed by fine root tips of plants and ectomycorrhizal fungi, has a wide range of effects on forest ecology. Species of Keteleeria Carrière (Pinaceae, Gymnosperms), endemic to East and Southeast Asia, are important for ecology and forest economics. However, lots of natural populations of Keteleeria have entered a recession. ECM fungi can improve seedling survival rate and contribute to the recovery of plant population. In this study, the diversity of ECM fungi associated with K. fortunei var. cyclolepis and K. davidiana were investigated by sequencing the fungal DNA barcoding fragment. With the available data of K. fortune and K. evelyniana integrated, the factors potentially driving the ECM fungal communities associated with Keteleeria spp. were investigated. The main results are as follows: 1. The diversity of ECM fungi associated with K. fortunei var. cyclolepis and K. davidiana A total of 551 sequences of ECM fungi were sequenced. Among these, 364 sequences are from K. fortunei var. cyclolepi, and 187 sequences are from K. davidiana. These sequences were grouped into 100 OTUs (operational taxonomic unit, OTU) by a 97% threshold, of which 72 OTUs are associated with K. fortunei var. cyclolepis and 46 OTUs are associated with K. davidiana. These OTUs were members of Basidiomycetes (84 OTUs) and Ascomycetes (16 OTUs). 89 OTUs were from 22 lineages. Russula, represented by 185 sequences, is the dominant genus with an important value of 70.72%, followed by Tomentella and Cenococcum with important value of 37.98% and 33.18% respectively. Russula, Cenococcum, Tomentella, Wilcoxina, Sebacina, shaped the ECM fungi community differentiation between samples. 2. High diversity and differentiation of ECM fungi associated with Keteleeria spp. A total of 36 sites from Yunnan, Guizhou, Guangxi, Guangdong, Fujian, Hubei, Sichuan, were surveyed. A total of 1606 sequences of ECM fungi were included. These sequences were grouped into 222 OTUs from 23 lineages. /russula-lactarius had the largest number of OTUs (67 OTUs) and /boletus contains the largest number of genera (7 genera). According to the geographic ordination and identification based on the ITS sequences, Keteleeria spp., the host plants, were clustered into two groups, the eastern group and western group. Among the 23 ECM fungal lineages, 16 are shared by the eastern and western groups; two lineages were unique in the eastern group, /pisolithus-scleroderma and /albatrellus. 5 lineages were unique in the western group, /cortinarius, /hebeloma-alnicola, /marcelleina-peziza gerardii, /pseudotomentella, /tricholoma. Both groups displayed high diversity of ECM fungi, and the diversity of eastern group was higher than that of western group, with 153 OTUs for the eastern group and 126 OTUs for the western group. Significant differences of ECM fungal diversity between the two groups were detected by ANOSIM analysis (P = 0.048). Species replacement was the main pattern manifes
	2021-05
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74625
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	许天修. 油杉属(Keteleeria)植物外生菌根真菌多样性、群落组成及其影响因素研究, Diversity, community composition and driving factors of ectomycorrhizal fungi associated with Keteleeria[D],2021.

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