丫蕊花中水溶性皂苷的制备及其活性评价

	丫蕊花中水溶性皂苷的制备及其活性评价; Preparation and bioactivity evaluation of water-soluble saponins from Ypsilandra thibetica
	张仕娟
导师	刘海洋
摘要	Ypsilandra thibetica, belonging to the genus Ypsilandra (Melanthiaceae family), has been recorded in Chinese Materia Medica (1999 Edition) with the pharmaceutical functions of clearing away heat and toxic materials, removing stasis, and prompting diuresis. In the previous research of our research group, we found that the ethanol extract of Y. thibetica showed significant activities of inducing platelet aggregation and promoting contraction of rat uterus in vitro. Furthermore, its main active ingredients were basically clarified and water-soluble part (the 40% EtOH eluate) with a mass of more than 20% of the total extract after passing through a macroporous resin column had no hemostatic and uterine contraction-promoting activities. In order to realize the comprehensive utilization of the Y. thibetica resource, a variety of bioactive screenings of the water-soluble part were carried out. Fortunately, we found it showed certain inhibitory activities against plant pathogenic fungi. In order to clarify the material basis of its antifungal activity, phytochemical research and bioactive screening were carried out. This thesis consists of two chapters. In the first chapter, the antifungal activities of water-soluble part against three common plant pathogenic fungi Botrytis cinerea, Colletotrichum nymphaeae and Alternaria alternata were evaluated. The results showed the water-soluble part was most effective against B. cinerea with an inhibition ratio of 74.64 ± 3.24% at the concentration of 100 ppm. In order to obtain the active compounds, a study for the separation and structure identification of the water-soluble constituents of Y. thibetica was carried out. A total of 31 steroidal saponins, including 10 new ones, were isolated and identified by means of comprehensive chromatography methods, such as macroporous adsorption resin, silica gel column chromatography, reversed phase Rp-18, Sephadex LH-20 and semi-preparative HPLC, combined with modern spectroscopic techniques, such as NMR, MS, and IR. According to the structural features of the aglycone, these compounds can be divided into furostane-type (1?5 and 11?17), spirostane-type (6 and 18?24), cholestane-type (7 and 25-27), pregnane-type (8?9 and 28?30), and C22 steroid-(22, 16)-lactone (10 and 31) steroidal saponins. As for the sugar chains, they are made up mainly by β-D-glucopyranosyl, α-L-rhamnopyranosyl, and β-D-apiofuranosyl. Among them, compounds 1?5 are new furostane steroidal saponins, 2 is the first C-21 aldehyded furostane steroidal saponin, 4 is the first furostane steroidal saponin with a C-22 and C-23 exocyclic double bond that is conjugated with a C-24 carbonyl group, 6 is a new spirostane steroidal saponin, 7 is a rare cholestane derivative with an aromatized E-ring bearing a carboxyl group, 8 and 9 are new pregnane steroidal saponins, and 10 is a new C22 steroid-(22,16)-lactone saponin. In the second chapter, the antifungal activities against B. cinerea of the major steroidal saponi
	2021-05
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74611
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	张仕娟. 丫蕊花中水溶性皂苷的制备及其活性评价, Preparation and bioactivity evaluation of water-soluble saponins from Ypsilandra thibetica[D],2021.

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