水平转移基因PpMACRO2在苔藓植物适应性进化中的作用

	水平转移基因PpMACRO2在苔藓植物适应性进化中的作用; Horizontally acquired PpMACRO2 gene in the adaptive evolution of mosses
	王双华
导师	孙航
摘要	Symbiosis between plants and fungi is critical for the evolution and adaptation of land plants. Given the intimate and long-term association between fungi and land plants, the exchange of nutrients and genetic material may occur between the two partners. Available studies have shown that genes might have been transferred from fungi to land plants. However, how the acquired fungal genes are involved in the adaptation and evolution of land plants remains obscure. In this study, we reported a macro2 domain gene, PpMACRO2, was likely acquired from mycorrhizae-like fungi by the ancestral land plant. This PpMACRO2 gene has been lost secondarily from vascular plants but is still retained in bryophytes. Macrodomains play a pivotal role in ADP-ribosylation, an important epigenetic modification mechanism. They regulate a number of cellular activities, such as chromatin modification, transcription and translation, DNA repair and cell differentiation. However, this mechanism is yet to be fully elucidated, and the role of macro2 domain has not been reported. In order to understand the roles of PpMACRO2 in the evolution and adaptation of early land plants, we generated knockout (ko), overexpression (OE) and EGFP-GUS knockin (KI) genetically modified plants of PpMACRO2, respectively, using the model plant Physcomitrium patens (formerly Physcomitrella patens) and through homologous recombination. In this study, the phenotype of the ko and OE mutants was observed and measured. Compared to the wild-type (WT), the ko mutants produced larger and fewer gametophores, whereas the OE lines produced smaller and more gametophores. In addition, the expression profile of PpMACRO2 was studied by using GUS and GFP signal of double-labeled plants in the lifecycle of P. patens, and the results indicate that PpMACRO2 is related to stem cell and three-dimensional gametophyte development. Furthermore, regeneration experiments of protoplast and detached leaves show that PpMACRO2 promotes cell reprogramming and tissue regeneration in P. patens. In addition, RNA-seq data for both ko and OE mutants and qRT-PCR data suggest that PpMACRO2 is not only involved in chromatin modification, but also regulates transcription factors AP2 and Homeobox that are involved in development. In lines with transcriptomic data, PpMACRO2 was also predicted to interact with histones H2A and H2B using STRING database.
	2021-05
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74584
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	王双华. 水平转移基因PpMACRO2在苔藓植物适应性进化中的作用, Horizontally acquired PpMACRO2 gene in the adaptive evolution of mosses[D],2021.

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