奶子藤属植物生物碱成分研究及其促进溶酶体生成活性评价

	奶子藤属植物生物碱成分研究及其促进溶酶体生成活性评价; Alkaloids from Bousigonia and Their Ability to Induce Lysosomal Biosynthesis.
	霍宗庆
导师	张于
摘要	Lysosome is not only an organelle for the degradation of intracellular substances, but also a key center of cell signal transduction and metabolic regulation. Its degradation and signal regulation functions do not conflict, but cooperate extensively to mediate basic cell activities. Lysosome-mediated signaling pathways and transcription programs can sense the state of cell metabolism and control the switch between anabolism and catabolism by regulating lysosomal biogenesis and autophagy. The generation and function maintenance of lysosomes are extremely important for the normal physiological function of cells. The dysfunction can lead to many types of major diseases, such as Lysosomal Storage Disorders (LSDs), about 70% of LSDs are manifested as neurodegenerative diseases (ND), such as Alzheimer's, Parkinson's and Huntington's diseases. Previous studies have shown that asidosperma-eburnea type and yohimbine type alkaloid can significantly promote lysosome formation, and these two types of alkaloids are mainly distributed in the Bousigonia plants of the Apocynaceae family. In order to expand the structural diversity of this compound and provide a sufficient material basis for further mechanism study, we selected two plants of genus Bousigonia (B. mekongensis and B. angustifolia) as research objects. The chemical constituents and their biological activities were studied, and related monomer alkaloids were used to synthesize dimer. In addition, a brief overview of the structure and activity of the bisindole dimers was reviewed in the past decade. The first chapter is about the alkaloid chemical constituents of B. angustifolia. By using a variety of separation methods, modern spectroscopy technology, X ray crystal diffraction analysis, and computational calculation, 32 alkaloids were isolated and identified from the dried branches and leaves of B. angustifolia collected from Xishuangbanna in Yunnan, mainly covering several groups of alkaloids such as asidosperma, eburnean, and yohimbine. Among them, five alkaloids are newcompounds, and the NMR data of two alkaloids are reported for the first time. Compound 1 (bousangine A) is a novel monoterpene indole alkaloid with a rearranged five-ring system. The second chapter is the research on the chemical constituents of B. mekongensis. By using a variety of separation methods, modern spectroscopy techniques, and computational chemistry, 20 alkaloids have been isolated and identified from the dried branches and leaves of B. mekongensis collected from Xishuangbanna, Yunnan. Among them, two are new compounds. The third chapter is the semi-synthesis study of aspidosperma-eburnea alkaloid dimer. Although the expected aspidosperma-eburnea alkaloid dimer could not be isolated from B. mekongensis and B. angustifolia, but the alkaloids of this type constitute monomers have been accumulated. Nine such alkaloid dimers were constructed by chemical methods.
	2020-08
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74135
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	霍宗庆. 奶子藤属植物生物碱成分研究及其促进溶酶体生成活性评价, Alkaloids from Bousigonia and Their Ability to Induce Lysosomal Biosynthesis.[D],2020.

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