湄公河流域灵芝的分类学、分子系统发育学及栽培研究

	湄公河流域灵芝的分类学、分子系统发育学及栽培研究; TAXONOMY, MOLECULAR PHYLOGENY, AND CULTIVATION OF Ganoderma SPECIES IN THE GREATER MEKONG SUBREGION
	Miss Thatsanee Luangharn
导师	许建初
摘要	Ganoderma P. Karst. belongs to the family Ganodermataceae, a unique group of polypore fungi. Ganoderma is distinctively characterized by its laccate or non-laccate basidiocarp, and double walls basidiospore. This taxon is commonly known as “Lingzhi” in Mandarin. Index Fungorum records 456 taxa, MycoBank records 398 taxa, and 80 species are estimated worldwide, though some are synonyms. Ganoderma is a cosmopolitan genus of mushrooms, many of which can cause root and butt rot diseases on many tree species. Members of this genus are particularly diverse across temperate and tropical regions. Many members of Ganoderma are used medicinally. Its uses in the treatment of human diseases and as a dietary supplement are common in traditional Asian medicine practices. In this study, I surveyed species of Ganoderma in the Greater Mekong Subregion (GMS), including those in the temperate climate of Yunnan Province, China, and in the tropical climate of Laos, Myanmar, Thailand and Vietnam. Samples are identified on the basis of macro- and micro-morphological characteristics and phylogenetic analyses. I recorded host tree species, substrate, climate, and coordinates at which the fruiting bodies were collected. Detailed morphologies of the specimens were recorded based on fresh material and documented photographically. Morphological characteristics such shape, size, colour and microscopic characteristics were free hand sections under a dissecting microscope and recorded in the laboratory. Phylogenetic analyses of combined ITS, LSU, RPB2 and TEF1α sequence data indicated that Ganoderma strains grouped in a distinct lineage in the genus. Their placement and closely related taxa are provided and discussed. Morphological characteristics are relatively easy to observe and record. Morphological observation has been used over the last 300 years to identify and infer relationships between fungi. Different methods of molecular analysis, especially DNA sequencing, has allowed for the reconstruction of evolutionary relationships across all organisms and led to a taxonomical renaissance. Because of high inter-species variation within Ganoderma, this fungus has been the subject of much debate by researchers. Thus, morphological characteristics and phylogenetic analysis are both critical to the project of fungal identification. In this comprehensive study, I introduce Ganoderma species from temperate and tropical regions of the GMS. I report 25 Ganoderma species from the GMS: G. adspersum, G. applanatum, G. australe, G. calidophilum, G. casuarinicola, G. ellipsoideum, G. flexipes, G. gibbosum, G. heohnelianum, G. hochiminhense, G. leucocontextum, G. lingzhi, G. lucidum, G. multiplicatum, G. multipileum, G. myanmarens, G. orbiforme, G. philippii, G. resinaceum, G. sinense, G. subresinosum, G. thailandicum, G. williamsianum, G. tropicum and G. tsugae. From Yunnan Province, China. I collected these 13 Ganoderma species: G. applanatum, G. australe, G. calidophilum, G. flexipes..
	2020-05
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74100
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	Miss Thatsanee Luangharn. 湄公河流域灵芝的分类学、分子系统发育学及栽培研究, TAXONOMY, MOLECULAR PHYLOGENY, AND CULTIVATION OF Ganoderma SPECIES IN THE GREATER MEKONG SUBREGION[D],2020.

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