Genetic Diversity and Population Structure Analysis of Wild Cymbidium tortisepalum Based on Chloroplast DNA in Yunnan Province of China

doi:10.21273/JASHS05046-21

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	Genetic Diversity and Population Structure Analysis of Wild Cymbidium tortisepalum Based on Chloroplast DNA in Yunnan Province of China
	Ma,Xiangli ; Tang,Min ; Bi,Yufen ; Yang,Junbo
	2021
发表期刊	JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE
ISSN	0003-1062
卷号	146 期号:6 页码:424-434
摘要	Cymbidium tortisepalum is a primary orchid species in Yunnan Province, China, and has an extremely high ornamental and economic value. To reveal the levels and distribution of genetic variation and structure of wild C. tortisepalum resources, sequence variations of six chloroplast DNA intergenic spacers (psbM-trnD, trnV-trnA, accD-psal, rrn23, trnk-rps16, and ycf1) were analyzed in 404 wild individuals from 28 populations in the three river area in Yunnan Province, China. The results showed that the six chloroplast DNA sequences were aligned with 61 polymorphic sites, including 50 indels and 11 haplotypes in 404 individuals, which revealed a low level of genetic diversity (total genetic diversity = 0.240, and the average value of nucleotide diversity = 0.00024). In addition, a fairly low genetic differentiation [coefficients for genetic differentiation among populations (G(ST)) = 0.099, number of substitution (N-ST) = 0.081] was found among the studied populations, and N-ST value was less than G(ST), which indicated that no significant phylogeographic structure existed in those populations. Furthermore, analysis of molecular variance revealed that great genetic variance (91%) came from individuals within the populations, which indicated that there was no clear genetic differentiation among populations. On the basis of these findings, a conservation plan was proposed to sample or preserve fewer populations but with more individuals from each population.
关键词	conservation genetic differentiation orchid three parallel rivers region MUTATION-RATE GENOME DIFFERENTIATION MITOCHONDRIAL ORCHIDACEAE SEQUENCES ELEVATION TESTS
DOI	10.21273/JASHS05046-21
WOS记录号	WOS:000729207400005
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73783
专题	中国科学院昆明植物研究所
作者单位	1.Yunnan Agr Univ, Coll Anim Sci & Technol, Kunming 650201, Yunnan, Peoples R China 2.Yunnan Agr Univ, Coll Hort & Landscape, Kunming 650201, Yunnan, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species Southwest China, Kunming 650204, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Ma,Xiangli,Tang,Min,Bi,Yufen,et al. Genetic Diversity and Population Structure Analysis of Wild Cymbidium tortisepalum Based on Chloroplast DNA in Yunnan Province of China[J]. JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE,2021,146(6):424-434.
APA	Ma,Xiangli,Tang,Min,Bi,Yufen,&Yang,Junbo.(2021).Genetic Diversity and Population Structure Analysis of Wild Cymbidium tortisepalum Based on Chloroplast DNA in Yunnan Province of China.JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE,146(6),424-434.
MLA	Ma,Xiangli,et al."Genetic Diversity and Population Structure Analysis of Wild Cymbidium tortisepalum Based on Chloroplast DNA in Yunnan Province of China".JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE 146.6(2021):424-434.