The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice

doi:10.1186/s12284-021-00531-7

KIB OpenIR

	The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice
	Hu,Jihong ; Huang,Liyu ; Chen,Guanglong ; Liu,Hui ; Zhang,Yesheng ; Zhang,Ru ; Zhang,Shilai ; Liu,Jintao ; Hu,Qingyi ; Hu,Fengyi ; Wang,Wen ; Ding,Yi
	2021
发表期刊	RICE
ISSN	1939-8425
卷号	14 期号:1 页码:90
摘要	Grain weight and grain number, the two important yield traits, are mainly determined by grain size and panicle architecture in rice. Herein, we report the identification and functional analysis of OsSPL4 in panicle and grain development of rice. Using CRISPR/Cas9 system, two elite alleles of OsSPL4 were obtained, which exhibited an increasing number of grains per panicle and grain size, resulting in increase of rice yield. Cytological analysis showed that OsSPL4 could regulate spikelet development by promoting cell division. The results of RNA-seq and qRT-PCR validations also demonstrated that several MADS-box and cell-cycle genes were up-regulated in the mutation lines. Co-expression network revealed that many yield-related genes were involved in the regulation network of OsSPL4. In addition, OsSPL4 could be cleaved by the osa-miR156 in vivo, and the OsmiR156-OsSPL4 module might regulate the grain size in rice. Further analysis indicated that the large-grain allele of OsSPL4 in indica rice might introgress from aus varieties under artificial selection. Taken together, our findings suggested that OsSPL4 could be as a key regulator of grain size by acting on cell division control and provided a strategy for panicle architecture and grain size modification for yield improvement in rice.
关键词	OsSPL4 Grain size Grain weight Panicle branching Grain yield Rice NATURAL VARIATION SBP-DOMAIN RARE ALLELE GENE BINDING IDENTIFICATION EXPRESSION PROTEIN DISSECTION PATHWAYS
DOI	10.1186/s12284-021-00531-7
WOS记录号	WOS:000714027400001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73718
专题	中国科学院昆明植物研究所
作者单位	1.Wuhan Univ, Coll Life Sci, State Key Lab Hybrid Rice, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming 650223, Yunnan, Peoples R China 3.Northwestern Polytech Univ, Sch Ecol & Environm, Xian 710072, Peoples R China 4.Yunnan Univ, Sch Agr, State Key Lab Conservat & Utilizat Bioresources Y, Kunming 650500, Yunnan, Peoples R China 5.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Yunnan, Peoples R China 6.BGI Baoshan, Baoshan 678004, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Hu,Jihong,Huang,Liyu,Chen,Guanglong,et al. The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice[J]. RICE,2021,14(1):90.
APA	Hu,Jihong.,Huang,Liyu.,Chen,Guanglong.,Liu,Hui.,Zhang,Yesheng.,...&Ding,Yi.(2021).The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice.RICE,14(1),90.
MLA	Hu,Jihong,et al."The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice".RICE 14.1(2021):90.