KIB OpenIR
Ketocarotenoids accumulation in the leaves of engineered Brassica napus restricts photosynthetic efficiency and plant growth
Wang,Jie-Lin; Tan,Shun-Ling; He,Ming-Xia; Huang,Wei; Huang,Jun-Chao
2021
Source PublicationENVIRONMENTAL AND EXPERIMENTAL BOTANY
ISSN0098-8472
Volume186Pages:104461
AbstractAstaxanthin is a high-valued ketocarotenoid that is rarely synthesized in higher plants due to their lack of a carotenoid ketolase. Overexpression of a heterologous 8-carotene ketolase gene coupled with other pathway genes in higher plants has triggered the production of various ketocarotenoids in the transformants. Here we reported that constitutive and simultaneous overexpression of four genes involved in astaxanthin biosynthesis in Brassica napus resulted in the accumulation of the nonnative ketocarotenoids such as astaxanthin and ketolutein, and the decreased production of chlorophylls, lutein and violaxanthin in leaves. As a result, transgenic plants showed lower non-photochemical quenching (NPQ) under high light, leading to moderate photoinhibition of photosystem II (PSII). Such PSII photoinhibition depressed linear electron transport and thus restricted the rate of CO2 assimilation. Consequently, transgenic plants grew slower and showed less biomass than wild type. These results indicated that endogenous synthesis of ketocarotenoids negatively affected photosynthesis and impaired plant growth in B. napus. Therefore, to prevent the side effect on normal growth, seed specific expression of the transgenes may be necessary for enhanced production of astaxanthin in seeds.
KeywordBrassica napus Astaxanthin Ketocarotenoids Photosystem II (PSII) Photoprotection HIGH-YIELD PRODUCTION PHOTOSYSTEM-II HAEMATOCOCCUS-PLUVIALIS ASTAXANTHIN BIOSYNTHESIS ELECTRON FLOW HUMAN HEALTH STRESS PHOTOINHIBITION CAROTENOIDS EXPRESSION
DOI10.1016/j.envexpbot.2021.104461
WOS IDWOS:000644943400006
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/73618
Collection中国科学院昆明植物研究所
Affiliation1.[Wang, Jie-Lin
2.Tan, Shun-Ling
3.He, Ming-Xia
4.Huang, Wei
5.Chinese Acad Sci, Kunming Inst Bot, Dept Econ Plants & Biotechnol, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China
6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
7.Shenzhen Univ, Inst Adv Study, Shenzhen 518000, Peoples R China
Recommended Citation
GB/T 7714
Wang,Jie-Lin,Tan,Shun-Ling,He,Ming-Xia,et al. Ketocarotenoids accumulation in the leaves of engineered Brassica napus restricts photosynthetic efficiency and plant growth[J]. ENVIRONMENTAL AND EXPERIMENTAL BOTANY,2021,186:104461.
APA Wang,Jie-Lin,Tan,Shun-Ling,He,Ming-Xia,Huang,Wei,&Huang,Jun-Chao.(2021).Ketocarotenoids accumulation in the leaves of engineered Brassica napus restricts photosynthetic efficiency and plant growth.ENVIRONMENTAL AND EXPERIMENTAL BOTANY,186,104461.
MLA Wang,Jie-Lin,et al."Ketocarotenoids accumulation in the leaves of engineered Brassica napus restricts photosynthetic efficiency and plant growth".ENVIRONMENTAL AND EXPERIMENTAL BOTANY 186(2021):104461.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang,Jie-Lin]'s Articles
[Tan,Shun-Ling]'s Articles
[He,Ming-Xia]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang,Jie-Lin]'s Articles
[Tan,Shun-Ling]'s Articles
[He,Ming-Xia]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang,Jie-Lin]'s Articles
[Tan,Shun-Ling]'s Articles
[He,Ming-Xia]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.