Ketocarotenoids accumulation in the leaves of engineered Brassica napus restricts photosynthetic efficiency and plant growth

doi:10.1016/j.envexpbot.2021.104461

KIB OpenIR

	Ketocarotenoids accumulation in the leaves of engineered Brassica napus restricts photosynthetic efficiency and plant growth
	Wang,Jie-Lin ; Tan,Shun-Ling ; He,Ming-Xia ; Huang,Wei; Huang,Jun-Chao
	2021
发表期刊	ENVIRONMENTAL AND EXPERIMENTAL BOTANY
ISSN	0098-8472
卷号	186 页码:104461
摘要	Astaxanthin is a high-valued ketocarotenoid that is rarely synthesized in higher plants due to their lack of a carotenoid ketolase. Overexpression of a heterologous 8-carotene ketolase gene coupled with other pathway genes in higher plants has triggered the production of various ketocarotenoids in the transformants. Here we reported that constitutive and simultaneous overexpression of four genes involved in astaxanthin biosynthesis in Brassica napus resulted in the accumulation of the nonnative ketocarotenoids such as astaxanthin and ketolutein, and the decreased production of chlorophylls, lutein and violaxanthin in leaves. As a result, transgenic plants showed lower non-photochemical quenching (NPQ) under high light, leading to moderate photoinhibition of photosystem II (PSII). Such PSII photoinhibition depressed linear electron transport and thus restricted the rate of CO2 assimilation. Consequently, transgenic plants grew slower and showed less biomass than wild type. These results indicated that endogenous synthesis of ketocarotenoids negatively affected photosynthesis and impaired plant growth in B. napus. Therefore, to prevent the side effect on normal growth, seed specific expression of the transgenes may be necessary for enhanced production of astaxanthin in seeds.
关键词	Brassica napus Astaxanthin Ketocarotenoids Photosystem II (PSII) Photoprotection HIGH-YIELD PRODUCTION PHOTOSYSTEM-II HAEMATOCOCCUS-PLUVIALIS ASTAXANTHIN BIOSYNTHESIS ELECTRON FLOW HUMAN HEALTH STRESS PHOTOINHIBITION CAROTENOIDS EXPRESSION
DOI	10.1016/j.envexpbot.2021.104461
WOS记录号	WOS:000644943400006
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73618
专题	中国科学院昆明植物研究所
作者单位	1.[Wang, Jie-Lin 2.Tan, Shun-Ling 3.He, Ming-Xia 4.Huang, Wei 5.Chinese Acad Sci, Kunming Inst Bot, Dept Econ Plants & Biotechnol, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.Shenzhen Univ, Inst Adv Study, Shenzhen 518000, Peoples R China
推荐引用方式 GB/T 7714	Wang,Jie-Lin,Tan,Shun-Ling,He,Ming-Xia,et al. Ketocarotenoids accumulation in the leaves of engineered Brassica napus restricts photosynthetic efficiency and plant growth[J]. ENVIRONMENTAL AND EXPERIMENTAL BOTANY,2021,186:104461.
APA	Wang,Jie-Lin,Tan,Shun-Ling,He,Ming-Xia,Huang,Wei,&Huang,Jun-Chao.(2021).Ketocarotenoids accumulation in the leaves of engineered Brassica napus restricts photosynthetic efficiency and plant growth.ENVIRONMENTAL AND EXPERIMENTAL BOTANY,186,104461.
MLA	Wang,Jie-Lin,et al."Ketocarotenoids accumulation in the leaves of engineered Brassica napus restricts photosynthetic efficiency and plant growth".ENVIRONMENTAL AND EXPERIMENTAL BOTANY 186(2021):104461.