Knowledge Management System of Kunming Institute of Botany,CAS
Ketocarotenoids accumulation in the leaves of engineered Brassica napus restricts photosynthetic efficiency and plant growth | |
Wang,Jie-Lin; Tan,Shun-Ling; He,Ming-Xia; Huang,Wei; Huang,Jun-Chao | |
2021 | |
发表期刊 | ENVIRONMENTAL AND EXPERIMENTAL BOTANY |
ISSN | 0098-8472 |
卷号 | 186页码:104461 |
摘要 | Astaxanthin is a high-valued ketocarotenoid that is rarely synthesized in higher plants due to their lack of a carotenoid ketolase. Overexpression of a heterologous 8-carotene ketolase gene coupled with other pathway genes in higher plants has triggered the production of various ketocarotenoids in the transformants. Here we reported that constitutive and simultaneous overexpression of four genes involved in astaxanthin biosynthesis in Brassica napus resulted in the accumulation of the nonnative ketocarotenoids such as astaxanthin and ketolutein, and the decreased production of chlorophylls, lutein and violaxanthin in leaves. As a result, transgenic plants showed lower non-photochemical quenching (NPQ) under high light, leading to moderate photoinhibition of photosystem II (PSII). Such PSII photoinhibition depressed linear electron transport and thus restricted the rate of CO2 assimilation. Consequently, transgenic plants grew slower and showed less biomass than wild type. These results indicated that endogenous synthesis of ketocarotenoids negatively affected photosynthesis and impaired plant growth in B. napus. Therefore, to prevent the side effect on normal growth, seed specific expression of the transgenes may be necessary for enhanced production of astaxanthin in seeds. |
关键词 | Brassica napus Astaxanthin Ketocarotenoids Photosystem II (PSII) Photoprotection HIGH-YIELD PRODUCTION PHOTOSYSTEM-II HAEMATOCOCCUS-PLUVIALIS ASTAXANTHIN BIOSYNTHESIS ELECTRON FLOW HUMAN HEALTH STRESS PHOTOINHIBITION CAROTENOIDS EXPRESSION |
DOI | 10.1016/j.envexpbot.2021.104461 |
WOS记录号 | WOS:000644943400006 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.kib.ac.cn/handle/151853/73618 |
专题 | 中国科学院昆明植物研究所 |
作者单位 | 1.[Wang, Jie-Lin 2.Tan, Shun-Ling 3.He, Ming-Xia 4.Huang, Wei 5.Chinese Acad Sci, Kunming Inst Bot, Dept Econ Plants & Biotechnol, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.Shenzhen Univ, Inst Adv Study, Shenzhen 518000, Peoples R China |
推荐引用方式 GB/T 7714 | Wang,Jie-Lin,Tan,Shun-Ling,He,Ming-Xia,et al. Ketocarotenoids accumulation in the leaves of engineered Brassica napus restricts photosynthetic efficiency and plant growth[J]. ENVIRONMENTAL AND EXPERIMENTAL BOTANY,2021,186:104461. |
APA | Wang,Jie-Lin,Tan,Shun-Ling,He,Ming-Xia,Huang,Wei,&Huang,Jun-Chao.(2021).Ketocarotenoids accumulation in the leaves of engineered Brassica napus restricts photosynthetic efficiency and plant growth.ENVIRONMENTAL AND EXPERIMENTAL BOTANY,186,104461. |
MLA | Wang,Jie-Lin,et al."Ketocarotenoids accumulation in the leaves of engineered Brassica napus restricts photosynthetic efficiency and plant growth".ENVIRONMENTAL AND EXPERIMENTAL BOTANY 186(2021):104461. |
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