Discovery and validation of quality markers of Fructus Aurantii against acetylcholinesterase using metabolomics and bioactivity assays

doi:10.1002/jssc.202001190

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	Discovery and validation of quality markers of Fructus Aurantii against acetylcholinesterase using metabolomics and bioactivity assays
	Wang,Yi-Kun ; Zhou,Zi-Meng ; Dai,Man-Yun ; Ma,Xiao-Fang ; Xiao,Xue-Rong ; Zhang,Shou-Wen ; Liu,Hong-Ning ; Li,Fei
	2021
发表期刊	JOURNAL OF SEPARATION SCIENCE
ISSN	1615-9306
卷号	44 期号:11 页码:2189-2205
摘要	Fructus Aurantii is a traditional medicated diet in East Asia. To determine the underlying chemical markers responsible for the quality and efficacy of Fructus Aurantii, a sensitive metabolomic method was applied to distinguish Fructus Aurantii in Jiangxi Province from other two geographical locations (Hunan Province and Chongqing City) in China. In the present study, multivariate analyses were adopted to compare chemical compositions in 21 batches of Fructus Aurantii samples. Among three geographical origins, 23 differential compounds were structurally identified. Serum pharmacochemistry exhibited that 22 components could be detected in rat serum. Six differential and absorbed components were selected as six potential markers. Statistical analysis revealed that the content of six markers varied widely in three origins of Fructus Aurantii. Six differential and absorbed components were evaluated further by biological activity. Neohesperidin, naringin, and meranzin showed inhibitory effect on acetylcholinesterase that regulates gastrointestinal motility in vitro and in silico, suggesting that these three components may be determined as the active biomarkers of Fructus Aurantii. These findings demonstrate the potential of biomarkers for identification and quality control of Fructus Aurantii.
关键词	Fructus Aurantii quality markers metabolomics serum pharmacochemistry ultra high performance liquid chromatography SERUM PHARMACOCHEMISTRY RAT PLASMA IDENTIFICATION METABOLITES IMMATURUS COMPONENTS EXTRACT NEOHESPERIDIN FINGERPRINT NARINGIN
DOI	10.1002/jssc.202001190
WOS记录号	WOS:000642261300001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73532
专题	中国科学院昆明植物研究所
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Jiangxi Univ Tradit Chinese Med, Academician Workstn, Nanchang, Jiangxi, Peoples R China 4.Jiangxi Univ Tradit Chinese Med, Res Ctr Nat Resources Chinese Med Mat & Ethn Med, Nanchang 33004, Jiangxi, Peoples R China 5.Sichuan Univ, West China Hosp, Frontiers Sci Ctr Dis Related Mol Network, Lab Metabol & Drug Induced Liver Injury, Chengdu, Peoples R China
推荐引用方式 GB/T 7714	Wang,Yi-Kun,Zhou,Zi-Meng,Dai,Man-Yun,et al. Discovery and validation of quality markers of Fructus Aurantii against acetylcholinesterase using metabolomics and bioactivity assays[J]. JOURNAL OF SEPARATION SCIENCE,2021,44(11):2189-2205.
APA	Wang,Yi-Kun.,Zhou,Zi-Meng.,Dai,Man-Yun.,Ma,Xiao-Fang.,Xiao,Xue-Rong.,...&Li,Fei.(2021).Discovery and validation of quality markers of Fructus Aurantii against acetylcholinesterase using metabolomics and bioactivity assays.JOURNAL OF SEPARATION SCIENCE,44(11),2189-2205.
MLA	Wang,Yi-Kun,et al."Discovery and validation of quality markers of Fructus Aurantii against acetylcholinesterase using metabolomics and bioactivity assays".JOURNAL OF SEPARATION SCIENCE 44.11(2021):2189-2205.

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